Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7
The resistance of <i>Escherichia coli</i> O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of <i>Cymbopogon citratus</i> (EO), have...
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MDPI AG
2020-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/3/102 |
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| author | Luis A. Ortega-Ramirez M. Melissa Gutiérrez-Pacheco Irasema Vargas-Arispuro Gustavo A. González-Aguilar Miguel A. Martínez-Téllez J. Fernando Ayala-Zavala |
| author_facet | Luis A. Ortega-Ramirez M. Melissa Gutiérrez-Pacheco Irasema Vargas-Arispuro Gustavo A. González-Aguilar Miguel A. Martínez-Téllez J. Fernando Ayala-Zavala |
| author_sort | Luis A. Ortega-Ramirez |
| collection | DOAJ |
| description | The resistance of <i>Escherichia coli</i> O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of <i>Cymbopogon citratus</i> (EO), have proven antibacterial activity against planktonic <i>E. coli</i>; however, no information was found about their efficacy and mode of action against <i>E. coli</i> biofilms. Therefore, the inhibitory effect of <i>C. citratus</i> EO, citral, and geraniol on glucans production and glucosyltransferase activity as anti-biofilm mechanism against <i>E. coli</i> was evaluated. EO, citral, and geraniol inhibited the planktonic growth of <i>E. coli</i> (minimal inhibitory concentration or MIC= 2.2, 1.0, and 3.0 mg/mL, respectively) and the bacterial adhesion (2.0, 2.0, and 4.0 mg/mL, respectively) on stainless steel. All compounds decreased the glucans production; citral and geraniol acted as uncompetitive inhibitors of glucosyltransferase activity (The half maximal inhibitory concentrations or IC<sub>50</sub> were 8.5 and 6.5 µM, respectively). The evidence collected by docking analysis indicated that both terpenes could interact with the helix finger of the glucosyltransferase responsible for the polymer production. In conclusion, <i>C. citratus</i> EO, citral, and geraniol inhibited glucosyltransferase activity, glucans production, and the consequent biofilm formation of <i>E. coli </i>O157:H7. |
| first_indexed | 2024-04-14T05:25:15Z |
| format | Article |
| id | doaj.art-582facdc5a034ce88c2cb7ac93b1cce1 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-04-14T05:25:15Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj.art-582facdc5a034ce88c2cb7ac93b1cce12022-12-22T02:09:59ZengMDPI AGAntibiotics2079-63822020-02-019310210.3390/antibiotics9030102antibiotics9030102Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7Luis A. Ortega-Ramirez0M. Melissa Gutiérrez-Pacheco1Irasema Vargas-Arispuro2Gustavo A. González-Aguilar3Miguel A. Martínez-Téllez4J. Fernando Ayala-Zavala5Centro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46. La Victoria, C. P. 83304. Hermosillo (83000), Sonora, MexicoThe resistance of <i>Escherichia coli</i> O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of <i>Cymbopogon citratus</i> (EO), have proven antibacterial activity against planktonic <i>E. coli</i>; however, no information was found about their efficacy and mode of action against <i>E. coli</i> biofilms. Therefore, the inhibitory effect of <i>C. citratus</i> EO, citral, and geraniol on glucans production and glucosyltransferase activity as anti-biofilm mechanism against <i>E. coli</i> was evaluated. EO, citral, and geraniol inhibited the planktonic growth of <i>E. coli</i> (minimal inhibitory concentration or MIC= 2.2, 1.0, and 3.0 mg/mL, respectively) and the bacterial adhesion (2.0, 2.0, and 4.0 mg/mL, respectively) on stainless steel. All compounds decreased the glucans production; citral and geraniol acted as uncompetitive inhibitors of glucosyltransferase activity (The half maximal inhibitory concentrations or IC<sub>50</sub> were 8.5 and 6.5 µM, respectively). The evidence collected by docking analysis indicated that both terpenes could interact with the helix finger of the glucosyltransferase responsible for the polymer production. In conclusion, <i>C. citratus</i> EO, citral, and geraniol inhibited glucosyltransferase activity, glucans production, and the consequent biofilm formation of <i>E. coli </i>O157:H7.https://www.mdpi.com/2079-6382/9/3/102extracellular polymeric substance matrixcellulose synthesisenzyme inhibitionessential oils |
| spellingShingle | Luis A. Ortega-Ramirez M. Melissa Gutiérrez-Pacheco Irasema Vargas-Arispuro Gustavo A. González-Aguilar Miguel A. Martínez-Téllez J. Fernando Ayala-Zavala Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 Antibiotics extracellular polymeric substance matrix cellulose synthesis enzyme inhibition essential oils |
| title | Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 |
| title_full | Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 |
| title_fullStr | Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 |
| title_full_unstemmed | Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 |
| title_short | Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against <i>Escherichia coli</i> O157:H7 |
| title_sort | inhibition of glucosyltransferase activity and glucan production as an antibiofilm mechanism of lemongrass essential oil against i escherichia coli i o157 h7 |
| topic | extracellular polymeric substance matrix cellulose synthesis enzyme inhibition essential oils |
| url | https://www.mdpi.com/2079-6382/9/3/102 |
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