The role of HSP60 in Atherosclerotic Coronary Heart disease
Background: Heat shock proteins have a general role in the response of the arterial wall to stress and may serve as a mediator/inducer of atherosclerosis in particular circumstances when HSPs specifically bind to the Toll-like receptor 4/CD14 complex, initiating an innate immune response, including...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2011-10-01
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| Series: | مجلة كلية الطب |
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| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/837 |
| _version_ | 1797369865616490496 |
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| author | Wurood A.S. Kadhim Nidhal A. Mohaymen |
| author_facet | Wurood A.S. Kadhim Nidhal A. Mohaymen |
| author_sort | Wurood A.S. Kadhim |
| collection | DOAJ |
| description | Background: Heat shock proteins have a general role in the response of the arterial wall to stress and may serve as a mediator/inducer of atherosclerosis in particular circumstances when HSPs specifically bind to the Toll-like receptor 4/CD14 complex, initiating an innate immune response, including the production of pro-inflammatory cytokines, this also followed by cytokine amplification through transmigration of macrophages and neutrophils.
Objective: To investigate the percentage of expression of HSP60 by peripheral blood lymphocyte (PBL) in atherosclerotic coronary heart disease (CHD) patients using immunocytochemistry technique.
Method: A total of fifty patient (40 males and 10 females), ranged from the mean age (59.12±8.54) years with a range from 42-80 years and fifteen, age and sex matched, apparently healthy individuals, taken as a healthy control group, were enrolled in the current study and the patients group were further classified into acute cases (n=11 patients) ״7 males and 4 females״ and chronic cases (n=39 patients) ״33 males and 6 females״. Three ml of blood sample had been taken from each subject, and immediately transferred to sterile heparinised vacutainer tubes for lymphocyte separation. Immunocytochemistry was used to detect HSP60 by using monoclonal antibody to HSP60 protein.
Result: Results revealed a statistically significant elevation levels between patients and control groups (P=0.000), the expression of HSP60 was elevated in all patients with no significant difference between acute and chronic cases (P>0.001).
Conclusions: HSP60 plays an important role in induction and development of atherosclerosis by stimulating different mechanisms which are the triggers for the process of atherosclerosis development. It is found in higher levels in both acute and chronic cases of atherosclerotic CHD in comparison with control cases. |
| first_indexed | 2024-03-08T17:54:11Z |
| format | Article |
| id | doaj.art-5836626719224f94b548cceb8e6b17b0 |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T17:54:11Z |
| publishDate | 2011-10-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-5836626719224f94b548cceb8e6b17b02024-01-02T06:09:57ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572011-10-0153310.32007/jfacmedbagdad.v3314-316%The role of HSP60 in Atherosclerotic Coronary Heart diseaseWurood A.S. Kadhim0Nidhal A. Mohaymen1Clinical Immunology, Dept. of Microbiology, College of Medicine, Al-Nahrain University.Clinical Immunology, Dept. of Microbiology, College of Medicine, Al-Nahrain University.Background: Heat shock proteins have a general role in the response of the arterial wall to stress and may serve as a mediator/inducer of atherosclerosis in particular circumstances when HSPs specifically bind to the Toll-like receptor 4/CD14 complex, initiating an innate immune response, including the production of pro-inflammatory cytokines, this also followed by cytokine amplification through transmigration of macrophages and neutrophils. Objective: To investigate the percentage of expression of HSP60 by peripheral blood lymphocyte (PBL) in atherosclerotic coronary heart disease (CHD) patients using immunocytochemistry technique. Method: A total of fifty patient (40 males and 10 females), ranged from the mean age (59.12±8.54) years with a range from 42-80 years and fifteen, age and sex matched, apparently healthy individuals, taken as a healthy control group, were enrolled in the current study and the patients group were further classified into acute cases (n=11 patients) ״7 males and 4 females״ and chronic cases (n=39 patients) ״33 males and 6 females״. Three ml of blood sample had been taken from each subject, and immediately transferred to sterile heparinised vacutainer tubes for lymphocyte separation. Immunocytochemistry was used to detect HSP60 by using monoclonal antibody to HSP60 protein. Result: Results revealed a statistically significant elevation levels between patients and control groups (P=0.000), the expression of HSP60 was elevated in all patients with no significant difference between acute and chronic cases (P>0.001). Conclusions: HSP60 plays an important role in induction and development of atherosclerosis by stimulating different mechanisms which are the triggers for the process of atherosclerosis development. It is found in higher levels in both acute and chronic cases of atherosclerotic CHD in comparison with control cases.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/837Atherosclerotic coronary heart disease, Heat shock proteins( HSP60) , immunocytochemistry technique. |
| spellingShingle | Wurood A.S. Kadhim Nidhal A. Mohaymen The role of HSP60 in Atherosclerotic Coronary Heart disease مجلة كلية الطب Atherosclerotic coronary heart disease, Heat shock proteins( HSP60) , immunocytochemistry technique. |
| title | The role of HSP60 in Atherosclerotic Coronary Heart disease |
| title_full | The role of HSP60 in Atherosclerotic Coronary Heart disease |
| title_fullStr | The role of HSP60 in Atherosclerotic Coronary Heart disease |
| title_full_unstemmed | The role of HSP60 in Atherosclerotic Coronary Heart disease |
| title_short | The role of HSP60 in Atherosclerotic Coronary Heart disease |
| title_sort | role of hsp60 in atherosclerotic coronary heart disease |
| topic | Atherosclerotic coronary heart disease, Heat shock proteins( HSP60) , immunocytochemistry technique. |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/837 |
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