The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis
This study investigated the intervention effects of Lactobacillus casei LC2W in murine (SPF C57BL/c) challenge infection models induced by Escherichia coli O157: H7. Mice were fed streptomycin with water for 3 days prior to intragastric gavage by E. coli O157:H7 (Control) or L. casei LC2W together w...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2020-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301373 |
| _version_ | 1797727995399503872 |
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| author | Guangqiang Wang Hongyu Tang Ying Zhang Xiang Xiao Yongjun Xia Lianzhong Ai |
| author_facet | Guangqiang Wang Hongyu Tang Ying Zhang Xiang Xiao Yongjun Xia Lianzhong Ai |
| author_sort | Guangqiang Wang |
| collection | DOAJ |
| description | This study investigated the intervention effects of Lactobacillus casei LC2W in murine (SPF C57BL/c) challenge infection models induced by Escherichia coli O157: H7. Mice were fed streptomycin with water for 3 days prior to intragastric gavage by E. coli O157:H7 (Control) or L. casei LC2W together with E. coli O157:H7 (Intervention) to explore the role of L. casei LC2W by biochemical indicators, histological evaluation, expression of colonic pro-inflammatory and intestinal barrier factors related to enteritis. Results showed that the administration of L. casei LC2W was able to alleviate the symptoms of colitis induced by E. coli O157:H7, exhibiting lower weight loss as well as more intact colon tissue. Furthermore, L. casei LC2W could down-regulate the expression of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) and protect the intestinal barrier function by improving the level of MUC2, ZO-1 and E-cadherin 1 compared to the control group. These results demonstrate that L. casei LC2W can reduce the severity of E. coli O157:H7 infection, and suggest L.casei LC2W may maintain the immune balance and intestinal barrier to reduce colitis. In addition, we found the effect of intervention is similar to that of prevention, which is better than that of treatment. |
| first_indexed | 2024-03-12T11:07:27Z |
| format | Article |
| id | doaj.art-5836b8694e73443c9d696c0b85bfa581 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T11:07:27Z |
| publishDate | 2020-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-5836b8694e73443c9d696c0b85bfa5812023-09-02T03:38:50ZengTsinghua University PressFood Science and Human Wellness2213-45302020-09-0193289294The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitisGuangqiang Wang0Hongyu Tang1Ying Zhang2Xiang Xiao3Yongjun Xia4Lianzhong Ai5Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Corresponding author at: University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China.This study investigated the intervention effects of Lactobacillus casei LC2W in murine (SPF C57BL/c) challenge infection models induced by Escherichia coli O157: H7. Mice were fed streptomycin with water for 3 days prior to intragastric gavage by E. coli O157:H7 (Control) or L. casei LC2W together with E. coli O157:H7 (Intervention) to explore the role of L. casei LC2W by biochemical indicators, histological evaluation, expression of colonic pro-inflammatory and intestinal barrier factors related to enteritis. Results showed that the administration of L. casei LC2W was able to alleviate the symptoms of colitis induced by E. coli O157:H7, exhibiting lower weight loss as well as more intact colon tissue. Furthermore, L. casei LC2W could down-regulate the expression of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) and protect the intestinal barrier function by improving the level of MUC2, ZO-1 and E-cadherin 1 compared to the control group. These results demonstrate that L. casei LC2W can reduce the severity of E. coli O157:H7 infection, and suggest L.casei LC2W may maintain the immune balance and intestinal barrier to reduce colitis. In addition, we found the effect of intervention is similar to that of prevention, which is better than that of treatment.http://www.sciencedirect.com/science/article/pii/S2213453020301373ProbioticsLactobacillus casei LC2WInterventionEscherichia coli O157:H7 |
| spellingShingle | Guangqiang Wang Hongyu Tang Ying Zhang Xiang Xiao Yongjun Xia Lianzhong Ai The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis Food Science and Human Wellness Probiotics Lactobacillus casei LC2W Intervention Escherichia coli O157:H7 |
| title | The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis |
| title_full | The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis |
| title_fullStr | The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis |
| title_full_unstemmed | The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis |
| title_short | The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157:H7 -induced mouse colitis |
| title_sort | intervention effects of lactobacillus casei lc2w on escherichia coli o157 h7 induced mouse colitis |
| topic | Probiotics Lactobacillus casei LC2W Intervention Escherichia coli O157:H7 |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301373 |
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