FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors.
BACKGROUND: The forkhead box transcription factor FOXQ1 has been shown to be upregulated in colorectal cancer (CRC) and metastatic breast cancer and involved in tumor development, epithelial-mesenchymal transition and chemoresistance. Yet, its transcriptional regulation is still unknown. METHODS: FO...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3608605?pdf=render |
| _version_ | 1819106841527844864 |
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| author | Jon Christensen Susanne Bentz Thierry Sengstag V Prasad Shastri Pascale Anderle |
| author_facet | Jon Christensen Susanne Bentz Thierry Sengstag V Prasad Shastri Pascale Anderle |
| author_sort | Jon Christensen |
| collection | DOAJ |
| description | BACKGROUND: The forkhead box transcription factor FOXQ1 has been shown to be upregulated in colorectal cancer (CRC) and metastatic breast cancer and involved in tumor development, epithelial-mesenchymal transition and chemoresistance. Yet, its transcriptional regulation is still unknown. METHODS: FOXQ1 mRNA and protein expression were analysed in a panel of CRC cell lines, and laser micro-dissected human biopsy samples by qRT-PCR, microarray GeneChip® U133 Plus 2.0 and western blots. FOXQ1 regulation was assayed by chromatin immunoprecipitation and luciferase reporter assays. RESULTS: FOXQ1 was robustly induced in CRC compared to other tumors, but had no predictive value with regards to grade, metastasis and survival in CRC. Prototype-based gene coexpression and gene set enrichment analysis showed a significant association between FOXQ1 and the Wnt pathway in tumors and cancer cell lines from different tissues. In vitro experiments confirmed, on a molecular level, FOXQ1 as a direct Wnt target. Analysis of known Wnt targets identified FOXQ1 as the most suitable marker for canonical Wnt activation across a wide panel of cell lines derived from different tissues. CONCLUSIONS: Our data show that FOXQ1 is one of the most over-expressed genes in CRC and a direct target of the canonical Wnt pathway. It is a potential new marker for detection of early CRC and Wnt activation in tumors of different origins. |
| first_indexed | 2024-12-22T02:44:33Z |
| format | Article |
| id | doaj.art-583771f745484d618531c03314d75d69 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T02:44:33Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-583771f745484d618531c03314d75d692022-12-21T18:41:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e6005110.1371/journal.pone.0060051FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors.Jon ChristensenSusanne BentzThierry SengstagV Prasad ShastriPascale AnderleBACKGROUND: The forkhead box transcription factor FOXQ1 has been shown to be upregulated in colorectal cancer (CRC) and metastatic breast cancer and involved in tumor development, epithelial-mesenchymal transition and chemoresistance. Yet, its transcriptional regulation is still unknown. METHODS: FOXQ1 mRNA and protein expression were analysed in a panel of CRC cell lines, and laser micro-dissected human biopsy samples by qRT-PCR, microarray GeneChip® U133 Plus 2.0 and western blots. FOXQ1 regulation was assayed by chromatin immunoprecipitation and luciferase reporter assays. RESULTS: FOXQ1 was robustly induced in CRC compared to other tumors, but had no predictive value with regards to grade, metastasis and survival in CRC. Prototype-based gene coexpression and gene set enrichment analysis showed a significant association between FOXQ1 and the Wnt pathway in tumors and cancer cell lines from different tissues. In vitro experiments confirmed, on a molecular level, FOXQ1 as a direct Wnt target. Analysis of known Wnt targets identified FOXQ1 as the most suitable marker for canonical Wnt activation across a wide panel of cell lines derived from different tissues. CONCLUSIONS: Our data show that FOXQ1 is one of the most over-expressed genes in CRC and a direct target of the canonical Wnt pathway. It is a potential new marker for detection of early CRC and Wnt activation in tumors of different origins.http://europepmc.org/articles/PMC3608605?pdf=render |
| spellingShingle | Jon Christensen Susanne Bentz Thierry Sengstag V Prasad Shastri Pascale Anderle FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. PLoS ONE |
| title | FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. |
| title_full | FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. |
| title_fullStr | FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. |
| title_full_unstemmed | FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. |
| title_short | FOXQ1, a novel target of the Wnt pathway and a new marker for activation of Wnt signaling in solid tumors. |
| title_sort | foxq1 a novel target of the wnt pathway and a new marker for activation of wnt signaling in solid tumors |
| url | http://europepmc.org/articles/PMC3608605?pdf=render |
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