Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine
The mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. Hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. Here we evaluated the capability of the fumonisin carboxylesterase F...
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MDPI AG
2016-03-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/3/84 |
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| author | Sabine Masching Karin Naehrer Heidi-Elisabeth Schwartz-Zimmermann Mihai Sărăndan Simone Schaumberger Ilse Dohnal Veronika Nagl Dian Schatzmayr |
| author_facet | Sabine Masching Karin Naehrer Heidi-Elisabeth Schwartz-Zimmermann Mihai Sărăndan Simone Schaumberger Ilse Dohnal Veronika Nagl Dian Schatzmayr |
| author_sort | Sabine Masching |
| collection | DOAJ |
| description | The mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. Hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. Here we evaluated the capability of the fumonisin carboxylesterase FumD to degrade FB1 to its less toxic metabolite hydrolyzed FB1 (HFB1) in the gastrointestinal tract of turkeys and pigs. First, an ex vivo pig model was used to examine the activity of FumD under digestive conditions. Within 2 h of incubation with FumD, FB1 was completely degraded to HFB1 in the duodenum and jejunum, respectively. To test the efficacy of the commercial application of FumD (FUMzyme) in vivo, female turkeys (n = 5) received either basal feed (CON), fumonisin-contaminated feed (15 mg/kg FB1+FB2; FB) or fumonisin-contaminated feed supplemented with FUMzyme (15 U/kg; FB+FUMzyme) for 14 days ad libitum. Addition of FUMzyme resulted in significantly decreased levels of FB1 in excreta, whereas HFB1 concentrations were significantly increased. Compared to the FB group (0.24 ± 0.02), the mean serum sphinganine-to-sphingosine (Sa/So) ratio was significantly reduced in the FB+FUMzyme group (0.19 ± 0.02), thus resembling values of the CON group (0.16 ± 0.02). Similarly, exposure of piglets (n = 10) to 2 mg/kg FB1+FB2 for 42 days caused significantly elevated serum Sa/So ratios (0.39 ± 0.15) compared to the CON group (0.14 ± 0.01). Supplementation with FUMzyme (60 U/kg) resulted in gastrointestinal degradation of FB1 and unaffected Sa/So ratios (0.16 ± 0.02). Thus, the carboxylesterase FumD represents an effective strategy to detoxify FB1 in the digestive tract of turkeys and pigs. |
| first_indexed | 2024-04-11T21:43:53Z |
| format | Article |
| id | doaj.art-583b9cf413b642e5a0e8d17a4301afaf |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T21:43:53Z |
| publishDate | 2016-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-583b9cf413b642e5a0e8d17a4301afaf2022-12-22T04:01:28ZengMDPI AGToxins2072-66512016-03-01838410.3390/toxins8030084toxins8030084Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and SwineSabine Masching0Karin Naehrer1Heidi-Elisabeth Schwartz-Zimmermann2Mihai Sărăndan3Simone Schaumberger4Ilse Dohnal5Veronika Nagl6Dian Schatzmayr7BIOMIN Holding GmbH, Erber Campus 1, 3131 Getzersdorf, AustriaBIOMIN Holding GmbH, Erber Campus 1, 3131 Getzersdorf, AustriaChristian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Str. 20, 3430 Tulln, AustriaFaculty of Veterinary Medicine Timișoara, Calea Aradului 119, 300645 Timișoara, RomaniaBIOMIN Holding GmbH, Erber Campus 1, 3131 Getzersdorf, AustriaBIOMIN Research Center, Technopark 1, 3430 Tulln, AustriaBIOMIN Research Center, Technopark 1, 3430 Tulln, AustriaBIOMIN Research Center, Technopark 1, 3430 Tulln, AustriaThe mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. Hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. Here we evaluated the capability of the fumonisin carboxylesterase FumD to degrade FB1 to its less toxic metabolite hydrolyzed FB1 (HFB1) in the gastrointestinal tract of turkeys and pigs. First, an ex vivo pig model was used to examine the activity of FumD under digestive conditions. Within 2 h of incubation with FumD, FB1 was completely degraded to HFB1 in the duodenum and jejunum, respectively. To test the efficacy of the commercial application of FumD (FUMzyme) in vivo, female turkeys (n = 5) received either basal feed (CON), fumonisin-contaminated feed (15 mg/kg FB1+FB2; FB) or fumonisin-contaminated feed supplemented with FUMzyme (15 U/kg; FB+FUMzyme) for 14 days ad libitum. Addition of FUMzyme resulted in significantly decreased levels of FB1 in excreta, whereas HFB1 concentrations were significantly increased. Compared to the FB group (0.24 ± 0.02), the mean serum sphinganine-to-sphingosine (Sa/So) ratio was significantly reduced in the FB+FUMzyme group (0.19 ± 0.02), thus resembling values of the CON group (0.16 ± 0.02). Similarly, exposure of piglets (n = 10) to 2 mg/kg FB1+FB2 for 42 days caused significantly elevated serum Sa/So ratios (0.39 ± 0.15) compared to the CON group (0.14 ± 0.01). Supplementation with FUMzyme (60 U/kg) resulted in gastrointestinal degradation of FB1 and unaffected Sa/So ratios (0.16 ± 0.02). Thus, the carboxylesterase FumD represents an effective strategy to detoxify FB1 in the digestive tract of turkeys and pigs.http://www.mdpi.com/2072-6651/8/3/84detoxificationbiotransformationfeed additiveFusarium toxinsbiomarkermetabolismtoxicokineticsswinepoultry |
| spellingShingle | Sabine Masching Karin Naehrer Heidi-Elisabeth Schwartz-Zimmermann Mihai Sărăndan Simone Schaumberger Ilse Dohnal Veronika Nagl Dian Schatzmayr Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine Toxins detoxification biotransformation feed additive Fusarium toxins biomarker metabolism toxicokinetics swine poultry |
| title | Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine |
| title_full | Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine |
| title_fullStr | Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine |
| title_full_unstemmed | Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine |
| title_short | Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine |
| title_sort | gastrointestinal degradation of fumonisin b1 by carboxylesterase fumd prevents fumonisin induced alteration of sphingolipid metabolism in turkey and swine |
| topic | detoxification biotransformation feed additive Fusarium toxins biomarker metabolism toxicokinetics swine poultry |
| url | http://www.mdpi.com/2072-6651/8/3/84 |
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