Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice
As a common emerging environmental pollutant, microplastics (MPs) have been detected in a variety of environmental media and human bodies. The potential toxic effects and mechanisms of MPs need to be revealed urgently. MPs can be deposited in the kidney, and exposure to high doses of MPs can cause n...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/20/7104 |
| _version_ | 1797572771205611520 |
|---|---|
| author | Ting Shen Wenjing Zhang Yirun Wang Haizhu Li Jingwei Wu Qian Wang Li Qin Lu Zhang Cuiqing Liu Ran Li |
| author_facet | Ting Shen Wenjing Zhang Yirun Wang Haizhu Li Jingwei Wu Qian Wang Li Qin Lu Zhang Cuiqing Liu Ran Li |
| author_sort | Ting Shen |
| collection | DOAJ |
| description | As a common emerging environmental pollutant, microplastics (MPs) have been detected in a variety of environmental media and human bodies. The potential toxic effects and mechanisms of MPs need to be revealed urgently. MPs can be deposited in the kidney, and exposure to high doses of MPs can cause nephrotoxicity in experimental animals. In this study, we investigated the effects of exposure to polystyrene microplastics (PS-MPs) at environmentally relevant doses (0.1 and 1 mg/L) on kidney structure, function, and transcriptome in mice. We found that mice exposed to PS-MPs in drinking water for eight weeks had no change in body weight or kidney coefficient. PS-MPs administration decreased the levels of blood urea nitrogen (BUN) in mice, while serum creatinine (CRE) and uric acid (UA) concentrations were unaffected. Through using periodic acid–Schiff (PAS) and Masson staining, we discovered that the glomerular tuft area increased in the PS-MP-treated mice, while the degree of renal fibrosis remained unchanged. Furthermore, renal cortex transcriptomic analysis identified 388 and 303 differentially expressed genes (DEGs) in the 0.1 and 1 mg/L dose groups, respectively. The DEGs were highly enriched in mitochondrial-related terms and pathways of thermogenesis and oxidative phosphorylation. Moreover, protein–protein interaction (PPI) network analysis revealed that cytochrome b-c1 complex subunit 10 (UQCR11) and cytochrome c oxidase subunit 3 (MT-CO3) were important node proteins. These findings suggest that environmental exposure to MPs can cause abnormalities in renal structure and filtration function and that long-term exposure to MPs may be a risk factor for renal disease. |
| first_indexed | 2024-03-10T21:00:36Z |
| format | Article |
| id | doaj.art-583d3ef653cd4491b167283223a6d64b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:00:36Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-583d3ef653cd4491b167283223a6d64b2023-11-19T17:32:48ZengMDPI AGMolecules1420-30492023-10-012820710410.3390/molecules28207104Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in MiceTing Shen0Wenjing Zhang1Yirun Wang2Haizhu Li3Jingwei Wu4Qian Wang5Li Qin6Lu Zhang7Cuiqing Liu8Ran Li9School of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Public Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaAs a common emerging environmental pollutant, microplastics (MPs) have been detected in a variety of environmental media and human bodies. The potential toxic effects and mechanisms of MPs need to be revealed urgently. MPs can be deposited in the kidney, and exposure to high doses of MPs can cause nephrotoxicity in experimental animals. In this study, we investigated the effects of exposure to polystyrene microplastics (PS-MPs) at environmentally relevant doses (0.1 and 1 mg/L) on kidney structure, function, and transcriptome in mice. We found that mice exposed to PS-MPs in drinking water for eight weeks had no change in body weight or kidney coefficient. PS-MPs administration decreased the levels of blood urea nitrogen (BUN) in mice, while serum creatinine (CRE) and uric acid (UA) concentrations were unaffected. Through using periodic acid–Schiff (PAS) and Masson staining, we discovered that the glomerular tuft area increased in the PS-MP-treated mice, while the degree of renal fibrosis remained unchanged. Furthermore, renal cortex transcriptomic analysis identified 388 and 303 differentially expressed genes (DEGs) in the 0.1 and 1 mg/L dose groups, respectively. The DEGs were highly enriched in mitochondrial-related terms and pathways of thermogenesis and oxidative phosphorylation. Moreover, protein–protein interaction (PPI) network analysis revealed that cytochrome b-c1 complex subunit 10 (UQCR11) and cytochrome c oxidase subunit 3 (MT-CO3) were important node proteins. These findings suggest that environmental exposure to MPs can cause abnormalities in renal structure and filtration function and that long-term exposure to MPs may be a risk factor for renal disease.https://www.mdpi.com/1420-3049/28/20/7104microplasticsmicenephrotoxicitytranscriptome |
| spellingShingle | Ting Shen Wenjing Zhang Yirun Wang Haizhu Li Jingwei Wu Qian Wang Li Qin Lu Zhang Cuiqing Liu Ran Li Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice Molecules microplastics mice nephrotoxicity transcriptome |
| title | Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice |
| title_full | Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice |
| title_fullStr | Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice |
| title_full_unstemmed | Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice |
| title_short | Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice |
| title_sort | effects of microplastic mp exposure at environmentally relevant doses on the structure function and transcriptome of the kidney in mice |
| topic | microplastics mice nephrotoxicity transcriptome |
| url | https://www.mdpi.com/1420-3049/28/20/7104 |
| work_keys_str_mv | AT tingshen effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT wenjingzhang effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT yirunwang effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT haizhuli effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT jingweiwu effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT qianwang effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT liqin effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT luzhang effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT cuiqingliu effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice AT ranli effectsofmicroplasticmpexposureatenvironmentallyrelevantdosesonthestructurefunctionandtranscriptomeofthekidneyinmice |