Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing
<i>Gypsophila paniculata</i> is the only species in the genus <i>Gypsophila</i> that has been used as cut flowers, and the sequencing of its genome has just been completed, opening a new chapter in its molecular genetic breeding. The molecular marker system is the basis for g...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/10/921 |
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| author | Chunlian Jin Bin Liu Jiwei Ruan Chunmei Yang Fan Li |
| author_facet | Chunlian Jin Bin Liu Jiwei Ruan Chunmei Yang Fan Li |
| author_sort | Chunlian Jin |
| collection | DOAJ |
| description | <i>Gypsophila paniculata</i> is the only species in the genus <i>Gypsophila</i> that has been used as cut flowers, and the sequencing of its genome has just been completed, opening a new chapter in its molecular genetic breeding. The molecular marker system is the basis for genetic molecular research in the era of genomics, whereas it is still a gap for <i>G. paniculata</i>. In this study, we constructed a genome-wide InDel marker system of <i>G. paniculata</i> after genome resequencing of another wild-type accession with white flowers. Consequently, 407 InDel markers at a distance of ~2 Mb were designed for all 17 chromosomes. Later, the validation of these markers by PCR revealed that 289 markers could distinguish alleles of the two wild-type alleles clearly. The predicted polymorphisms of two wild-type alleles were then transferred to the commercial cultivars, which displayed a rich polymorphism among four commercial cultivars. Our research established the first genome-level genetic map in <i>G. paniculata</i>, providing a comprehensive set of marker systems for its molecular research. |
| first_indexed | 2024-03-09T20:08:38Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T20:08:38Z |
| publishDate | 2022-10-01 |
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| series | Horticulturae |
| spelling | doaj.art-583d893d417f4b89a290ea05d2ae59d62023-11-24T00:23:22ZengMDPI AGHorticulturae2311-75242022-10-0181092110.3390/horticulturae8100921Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome ResequencingChunlian Jin0Bin Liu1Jiwei Ruan2Chunmei Yang3Fan Li4Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Kunming 650205, ChinaSchool of Agriculture, Yunnan University, Kunming 650500, ChinaFloriculture Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Kunming 650205, ChinaFloriculture Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Kunming 650205, ChinaFloriculture Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Kunming 650205, China<i>Gypsophila paniculata</i> is the only species in the genus <i>Gypsophila</i> that has been used as cut flowers, and the sequencing of its genome has just been completed, opening a new chapter in its molecular genetic breeding. The molecular marker system is the basis for genetic molecular research in the era of genomics, whereas it is still a gap for <i>G. paniculata</i>. In this study, we constructed a genome-wide InDel marker system of <i>G. paniculata</i> after genome resequencing of another wild-type accession with white flowers. Consequently, 407 InDel markers at a distance of ~2 Mb were designed for all 17 chromosomes. Later, the validation of these markers by PCR revealed that 289 markers could distinguish alleles of the two wild-type alleles clearly. The predicted polymorphisms of two wild-type alleles were then transferred to the commercial cultivars, which displayed a rich polymorphism among four commercial cultivars. Our research established the first genome-level genetic map in <i>G. paniculata</i>, providing a comprehensive set of marker systems for its molecular research.https://www.mdpi.com/2311-7524/8/10/921<i>Gypsophila paniculata</i>InDel markergenetic mappolymorphism |
| spellingShingle | Chunlian Jin Bin Liu Jiwei Ruan Chunmei Yang Fan Li Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing Horticulturae <i>Gypsophila paniculata</i> InDel marker genetic map polymorphism |
| title | Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing |
| title_full | Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing |
| title_fullStr | Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing |
| title_full_unstemmed | Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing |
| title_short | Development of InDel Markers for <i>Gypsophila paniculata</i> Based on Genome Resequencing |
| title_sort | development of indel markers for i gypsophila paniculata i based on genome resequencing |
| topic | <i>Gypsophila paniculata</i> InDel marker genetic map polymorphism |
| url | https://www.mdpi.com/2311-7524/8/10/921 |
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