Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo
Cheongchunchal (CE) is a developed crop more highly enriched in cyanidin-3-O-glucoside chloride (anthocyanin) than conventional waxy corn. Anthocyanin has been proven to have anti-oxidant, anti-inflammatory, anti-obesity, and anti-cancer effects. In this study, using high-performance liquid chromato...
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MDPI AG
2020-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/11/3453 |
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| author | Hye Won Kawk Gun-He Nam Myeong Jin Kim Sang-Yong Kim Gi No Kim Young-Min Kim |
| author_facet | Hye Won Kawk Gun-He Nam Myeong Jin Kim Sang-Yong Kim Gi No Kim Young-Min Kim |
| author_sort | Hye Won Kawk |
| collection | DOAJ |
| description | Cheongchunchal (CE) is a developed crop more highly enriched in cyanidin-3-O-glucoside chloride (anthocyanin) than conventional waxy corn. Anthocyanin has been proven to have anti-oxidant, anti-inflammatory, anti-obesity, and anti-cancer effects. In this study, using high-performance liquid chromatography (HPLC), Cheongchunchal was confirmed to contain 8.99 mg/g anthocyanin. The inhibitory effect of an ethanol extract of Cheongchunchal (CE) on adipocyte differentiation was demonstrated using Oil Red O staining and a triglyceride assay. By conducting Western blotting, we also confirmed the regulatory effect of CE on adipocyte differentiation factors by assessing changes in the levels of factors that play a significant role in the differentiation of 3T3-L1 preadipocytes. A C57BL/6N mouse model of obesity was induced with a high-fat diet, and CE (400, 600, and 800 mg/kg/day) or Garcinia (245 mg/kg/day) was orally administered to verify the anti-obesity effect of CE. As a result of CE administration, the food efficiency ratio (FER), weight gain, and weight of tissues decreased. Additionally, blood biochemical changes were observed. Furthermore, the inhibitory effect of CE on adipocytes was confirmed through morphological observation and the expression of adipocyte differentiation-related factors in the liver and fat tissues. Therefore, in this study, we verified the anti-obesity effects of anthocyanin-rich CE both in vitro and in vivo. |
| first_indexed | 2024-03-10T14:56:50Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T14:56:50Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-583db46b7c324da4bf8d387d4dd3a0e02023-11-20T20:32:58ZengMDPI AGNutrients2072-66432020-11-011211345310.3390/nu12113453Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In VivoHye Won Kawk0Gun-He Nam1Myeong Jin Kim2Sang-Yong Kim3Gi No Kim4Young-Min Kim5Department of Biological Science and Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 34054, KoreaDepartment of Biological Science and Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 34054, KoreaDepartment of Biological Science and Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 34054, KoreaDepartment of Food Science and Bio Technology, Shinansan University, Ansan 15435, KoreaPharmtech Korea LTD., 59, Munmakgongdan-gil, Munmak-eup, Wonju-si, Gangwon-do 26362, KoreaDepartment of Biological Science and Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 34054, KoreaCheongchunchal (CE) is a developed crop more highly enriched in cyanidin-3-O-glucoside chloride (anthocyanin) than conventional waxy corn. Anthocyanin has been proven to have anti-oxidant, anti-inflammatory, anti-obesity, and anti-cancer effects. In this study, using high-performance liquid chromatography (HPLC), Cheongchunchal was confirmed to contain 8.99 mg/g anthocyanin. The inhibitory effect of an ethanol extract of Cheongchunchal (CE) on adipocyte differentiation was demonstrated using Oil Red O staining and a triglyceride assay. By conducting Western blotting, we also confirmed the regulatory effect of CE on adipocyte differentiation factors by assessing changes in the levels of factors that play a significant role in the differentiation of 3T3-L1 preadipocytes. A C57BL/6N mouse model of obesity was induced with a high-fat diet, and CE (400, 600, and 800 mg/kg/day) or Garcinia (245 mg/kg/day) was orally administered to verify the anti-obesity effect of CE. As a result of CE administration, the food efficiency ratio (FER), weight gain, and weight of tissues decreased. Additionally, blood biochemical changes were observed. Furthermore, the inhibitory effect of CE on adipocytes was confirmed through morphological observation and the expression of adipocyte differentiation-related factors in the liver and fat tissues. Therefore, in this study, we verified the anti-obesity effects of anthocyanin-rich CE both in vitro and in vivo.https://www.mdpi.com/2072-6643/12/11/3453anti-obesityCheongchunchalanthocyanin3T3-L1 preadipocytesC57BL/6N model |
| spellingShingle | Hye Won Kawk Gun-He Nam Myeong Jin Kim Sang-Yong Kim Gi No Kim Young-Min Kim Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo Nutrients anti-obesity Cheongchunchal anthocyanin 3T3-L1 preadipocytes C57BL/6N model |
| title | Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo |
| title_full | Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo |
| title_fullStr | Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo |
| title_full_unstemmed | Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo |
| title_short | Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo |
| title_sort | anti obesity effect of an ethanol extract of cheongchunchal in vitro and in vivo |
| topic | anti-obesity Cheongchunchal anthocyanin 3T3-L1 preadipocytes C57BL/6N model |
| url | https://www.mdpi.com/2072-6643/12/11/3453 |
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