Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources
Abstract This study aimed to evaluate the efficacy of magnetic nanocomposite of cefotax against MRSA. A total of 190 samples were collected from milk, farm personnel and different environmental components from the dairy farm under the study to isolate S. aureus. Cefotax based magnetic nanoparticles...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-04160-4 |
| _version_ | 1819008473659080704 |
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| author | Manar Bahaa El Din Mohamed Fatma I. Abo El-Ela Rehab K. Mahmoud Ahmed A. Farghali Shymaa Gamil Sahar Abdel Aleem Abdel Aziz |
| author_facet | Manar Bahaa El Din Mohamed Fatma I. Abo El-Ela Rehab K. Mahmoud Ahmed A. Farghali Shymaa Gamil Sahar Abdel Aleem Abdel Aziz |
| author_sort | Manar Bahaa El Din Mohamed |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the efficacy of magnetic nanocomposite of cefotax against MRSA. A total of 190 samples were collected from milk, farm personnel and different environmental components from the dairy farm under the study to isolate S. aureus. Cefotax based magnetic nanoparticles was synthetized by the adsorption method and marked using Fourier-transform infrared spectrum (FT-IR), and X-ray diffraction (XRD), then it was characterized using Scanning and Transmission Electron Microscope (SEM and TEM). The obtained results revealed that number of positive samples of S. aureus isolation were 63 (33.1%), mainly from feed manger followed by milk machine swabs (60.0 and 53.3%, respectively) at X2 = 48.83 and P < 0.001. Obtained isolates were identified biochemically and by using molecular assays (PCR), also mec A gene responsible for resistance to cefotax was detected. Testing the sensitivity of 63 isolates of S. aureus showed variable degree of resistance to different tested antibiotics and significant sensitivity to cefotax based magnetic nanoparticles at P < 0.05. It was concluded that dairy environment might act a potential source for transmission of MRSA between human and animal populations. In addition, cefotax based magnetic nanoparticles verified an extreme antimicrobial efficacy against MRSA. |
| first_indexed | 2024-12-21T00:41:02Z |
| format | Article |
| id | doaj.art-583f7353428e413395694be387962200 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T00:41:02Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-583f7353428e413395694be3879622002022-12-21T19:21:41ZengNature PortfolioScientific Reports2045-23222022-01-0112111010.1038/s41598-021-04160-4Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sourcesManar Bahaa El Din Mohamed0Fatma I. Abo El-Ela1Rehab K. Mahmoud2Ahmed A. Farghali3Shymaa Gamil4Sahar Abdel Aleem Abdel Aziz5Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef UniversityAssociate professor of Pharmacology, Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef UniversityDepartment of Chemistry, Faculty of Science, Beni-Suef UniversityDepartment of Materials Science and Nanotechnology, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef UniversityDepartment of Materials Science and Nanotechnology, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef UniversityDepartment of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef UniversityAbstract This study aimed to evaluate the efficacy of magnetic nanocomposite of cefotax against MRSA. A total of 190 samples were collected from milk, farm personnel and different environmental components from the dairy farm under the study to isolate S. aureus. Cefotax based magnetic nanoparticles was synthetized by the adsorption method and marked using Fourier-transform infrared spectrum (FT-IR), and X-ray diffraction (XRD), then it was characterized using Scanning and Transmission Electron Microscope (SEM and TEM). The obtained results revealed that number of positive samples of S. aureus isolation were 63 (33.1%), mainly from feed manger followed by milk machine swabs (60.0 and 53.3%, respectively) at X2 = 48.83 and P < 0.001. Obtained isolates were identified biochemically and by using molecular assays (PCR), also mec A gene responsible for resistance to cefotax was detected. Testing the sensitivity of 63 isolates of S. aureus showed variable degree of resistance to different tested antibiotics and significant sensitivity to cefotax based magnetic nanoparticles at P < 0.05. It was concluded that dairy environment might act a potential source for transmission of MRSA between human and animal populations. In addition, cefotax based magnetic nanoparticles verified an extreme antimicrobial efficacy against MRSA.https://doi.org/10.1038/s41598-021-04160-4 |
| spellingShingle | Manar Bahaa El Din Mohamed Fatma I. Abo El-Ela Rehab K. Mahmoud Ahmed A. Farghali Shymaa Gamil Sahar Abdel Aleem Abdel Aziz Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources Scientific Reports |
| title | Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources |
| title_full | Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources |
| title_fullStr | Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources |
| title_full_unstemmed | Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources |
| title_short | Cefotax-magnetic nanoparticles as an alternative approach to control Methicillin-Resistant Staphylococcus aureus (MRSA) from different sources |
| title_sort | cefotax magnetic nanoparticles as an alternative approach to control methicillin resistant staphylococcus aureus mrsa from different sources |
| url | https://doi.org/10.1038/s41598-021-04160-4 |
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