Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize
The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 <i>ZmPum</i> genes were identified and classified into two groups in maize. Although...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14036 |
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| author | Wenqi Feng Hongwanjun Zhang Yang Cao Cheng Yang Muhammad Hayder Bin Khalid Qingqing Yang Wanchen Li Yingge Wang Fengling Fu Haoqiang Yu |
| author_facet | Wenqi Feng Hongwanjun Zhang Yang Cao Cheng Yang Muhammad Hayder Bin Khalid Qingqing Yang Wanchen Li Yingge Wang Fengling Fu Haoqiang Yu |
| author_sort | Wenqi Feng |
| collection | DOAJ |
| description | The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 <i>ZmPum</i> genes were identified and classified into two groups in maize. Although each ZmPum contains the conserved Pum domain, the ZmPum members show diversity in the gene and protein architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, and expression patterns. The typical ZmPum proteins have eight α-helices repeats, except for ZmPum2, 3, 5, 7, and 14, which have fewer α-helices. Moreover, we examined the expression profiles of <i>ZmPum</i> genes and found their involvement in kernel development. Except for <i>ZmPum2</i>, <i>ZmPum</i> genes are expressed in maize embryos, endosperms, or whole seeds. Notably, <i>ZmPum4</i>, <i>7</i>, and <i>13</i> exhibited dramatically high expression levels during seed development. The study not only contributes valuable information for further validating the functions of <i>ZmPum</i> genes but also provides insights for improvement and enhancing maize yield. |
| first_indexed | 2024-03-10T22:40:15Z |
| format | Article |
| id | doaj.art-58406fdbfe2b457e8c534ceb6da206fe |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:15Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-58406fdbfe2b457e8c534ceb6da206fe2023-11-19T11:06:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181403610.3390/ijms241814036Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in MaizeWenqi Feng0Hongwanjun Zhang1Yang Cao2Cheng Yang3Muhammad Hayder Bin Khalid4Qingqing Yang5Wanchen Li6Yingge Wang7Fengling Fu8Haoqiang Yu9Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaNational Research Centre of Intercropping, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaThe Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 <i>ZmPum</i> genes were identified and classified into two groups in maize. Although each ZmPum contains the conserved Pum domain, the ZmPum members show diversity in the gene and protein architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, and expression patterns. The typical ZmPum proteins have eight α-helices repeats, except for ZmPum2, 3, 5, 7, and 14, which have fewer α-helices. Moreover, we examined the expression profiles of <i>ZmPum</i> genes and found their involvement in kernel development. Except for <i>ZmPum2</i>, <i>ZmPum</i> genes are expressed in maize embryos, endosperms, or whole seeds. Notably, <i>ZmPum4</i>, <i>7</i>, and <i>13</i> exhibited dramatically high expression levels during seed development. The study not only contributes valuable information for further validating the functions of <i>ZmPum</i> genes but also provides insights for improvement and enhancing maize yield.https://www.mdpi.com/1422-0067/24/18/14036maizePumilio RNA-binding proteinsgene expressionkernel development |
| spellingShingle | Wenqi Feng Hongwanjun Zhang Yang Cao Cheng Yang Muhammad Hayder Bin Khalid Qingqing Yang Wanchen Li Yingge Wang Fengling Fu Haoqiang Yu Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize International Journal of Molecular Sciences maize Pumilio RNA-binding proteins gene expression kernel development |
| title | Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize |
| title_full | Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize |
| title_fullStr | Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize |
| title_full_unstemmed | Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize |
| title_short | Comprehensive Identification of the <i>Pum</i> Gene Family and Its Involvement in Kernel Development in Maize |
| title_sort | comprehensive identification of the i pum i gene family and its involvement in kernel development in maize |
| topic | maize Pumilio RNA-binding proteins gene expression kernel development |
| url | https://www.mdpi.com/1422-0067/24/18/14036 |
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