Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques
Different HIV vaccine regimens elicit distinct plasma antibody responses in both human and nonhuman primate models. Previous studies in human and non-human primate infants showed that adjuvants influenced the quality of plasma antibody responses induced by pediatric HIV envelope vaccine regimens. We...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719683/?tool=EBI |
| _version_ | 1819283590102384640 |
|---|---|
| author | Stella J. Berendam Papa K. Morgan-Asiedu Riley J. Mangan Shuk Hang Li Holly Heimsath Kan Luo Alan D. Curtis Joshua A. Eudailey Christopher B. Fox Mark A. Tomai Bonnie Phillips Hannah L. Itell Erika Kunz Michael Hudgens Kenneth Cronin Kevin Wiehe S. Munir Alam Koen K. A. Van Rompay Kristina De Paris Sallie R. Permar M. Anthony Moody Genevieve G. Fouda |
| author_facet | Stella J. Berendam Papa K. Morgan-Asiedu Riley J. Mangan Shuk Hang Li Holly Heimsath Kan Luo Alan D. Curtis Joshua A. Eudailey Christopher B. Fox Mark A. Tomai Bonnie Phillips Hannah L. Itell Erika Kunz Michael Hudgens Kenneth Cronin Kevin Wiehe S. Munir Alam Koen K. A. Van Rompay Kristina De Paris Sallie R. Permar M. Anthony Moody Genevieve G. Fouda |
| author_sort | Stella J. Berendam |
| collection | DOAJ |
| description | Different HIV vaccine regimens elicit distinct plasma antibody responses in both human and nonhuman primate models. Previous studies in human and non-human primate infants showed that adjuvants influenced the quality of plasma antibody responses induced by pediatric HIV envelope vaccine regimens. We recently reported that use of the 3M052-SE adjuvant and longer intervals between vaccinations are associated with higher magnitude of antibody responses in infant rhesus macaques. However, the impact of different adjuvants in HIV vaccine regimens on the developing infant B cell receptor (BCR) repertoire has not been studied. This study evaluated whether pediatric HIV envelope vaccine regimens with different adjuvants induced distinct antigen-specific memory B cell repertoires and whether specific immunoglobulin (Ig) immunogenetic characteristics are associated with higher magnitude of plasma antibody responses in vaccinated infant rhesus macaques. We utilized archived preclinical pediatric HIV vaccine studies PBMCs and tissue samples from 19 infant rhesus macaques immunized either with (i) HIV Env protein with a squalene adjuvant, (ii) MVA-HIV and Env protein co-administered using a 3-week interval, (iii) MVA-HIV prime/ protein boost with an extended 6-week interval between immunizations, or (iv) with HIV Env administered with 3M-052-SE adjuvant. Frequencies of vaccine-elicited HIV Env-specific memory B cells from PBMCs and tissues were similar across vaccination groups (frequency range of 0.06–1.72%). There was no association between vaccine-elicited antigen-specific memory B cell frequencies and plasma antibody titer or avidity. Moreover, the epitope specificity and Ig immunogenetic features of vaccine-elicited monoclonal antibodies did not differ between the different vaccine regimens. These data suggest that pediatric HIV envelope vaccine candidates with different adjuvants that previously induced higher magnitude and quality of plasma antibody responses in infant rhesus macaques were not driven by distinct antigen-specific memory BCR repertoires. |
| first_indexed | 2024-12-24T01:33:54Z |
| format | Article |
| id | doaj.art-5840ff20903c4d6daa40d78ff372e4d6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T01:33:54Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5840ff20903c4d6daa40d78ff372e4d62022-12-21T17:22:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011612Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaquesStella J. BerendamPapa K. Morgan-AsieduRiley J. ManganShuk Hang LiHolly HeimsathKan LuoAlan D. CurtisJoshua A. EudaileyChristopher B. FoxMark A. TomaiBonnie PhillipsHannah L. ItellErika KunzMichael HudgensKenneth CroninKevin WieheS. Munir AlamKoen K. A. Van RompayKristina De ParisSallie R. PermarM. Anthony MoodyGenevieve G. FoudaDifferent HIV vaccine regimens elicit distinct plasma antibody responses in both human and nonhuman primate models. Previous studies in human and non-human primate infants showed that adjuvants influenced the quality of plasma antibody responses induced by pediatric HIV envelope vaccine regimens. We recently reported that use of the 3M052-SE adjuvant and longer intervals between vaccinations are associated with higher magnitude of antibody responses in infant rhesus macaques. However, the impact of different adjuvants in HIV vaccine regimens on the developing infant B cell receptor (BCR) repertoire has not been studied. This study evaluated whether pediatric HIV envelope vaccine regimens with different adjuvants induced distinct antigen-specific memory B cell repertoires and whether specific immunoglobulin (Ig) immunogenetic characteristics are associated with higher magnitude of plasma antibody responses in vaccinated infant rhesus macaques. We utilized archived preclinical pediatric HIV vaccine studies PBMCs and tissue samples from 19 infant rhesus macaques immunized either with (i) HIV Env protein with a squalene adjuvant, (ii) MVA-HIV and Env protein co-administered using a 3-week interval, (iii) MVA-HIV prime/ protein boost with an extended 6-week interval between immunizations, or (iv) with HIV Env administered with 3M-052-SE adjuvant. Frequencies of vaccine-elicited HIV Env-specific memory B cells from PBMCs and tissues were similar across vaccination groups (frequency range of 0.06–1.72%). There was no association between vaccine-elicited antigen-specific memory B cell frequencies and plasma antibody titer or avidity. Moreover, the epitope specificity and Ig immunogenetic features of vaccine-elicited monoclonal antibodies did not differ between the different vaccine regimens. These data suggest that pediatric HIV envelope vaccine candidates with different adjuvants that previously induced higher magnitude and quality of plasma antibody responses in infant rhesus macaques were not driven by distinct antigen-specific memory BCR repertoires.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719683/?tool=EBI |
| spellingShingle | Stella J. Berendam Papa K. Morgan-Asiedu Riley J. Mangan Shuk Hang Li Holly Heimsath Kan Luo Alan D. Curtis Joshua A. Eudailey Christopher B. Fox Mark A. Tomai Bonnie Phillips Hannah L. Itell Erika Kunz Michael Hudgens Kenneth Cronin Kevin Wiehe S. Munir Alam Koen K. A. Van Rompay Kristina De Paris Sallie R. Permar M. Anthony Moody Genevieve G. Fouda Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques PLoS ONE |
| title | Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques |
| title_full | Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques |
| title_fullStr | Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques |
| title_full_unstemmed | Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques |
| title_short | Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques |
| title_sort | different adjuvanted pediatric hiv envelope vaccines induced distinct plasma antibody responses despite similar b cell receptor repertoires in infant rhesus macaques |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719683/?tool=EBI |
| work_keys_str_mv | AT stellajberendam differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT papakmorganasiedu differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT rileyjmangan differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT shukhangli differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT hollyheimsath differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT kanluo differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT alandcurtis differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT joshuaaeudailey differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT christopherbfox differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT markatomai differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT bonniephillips differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT hannahlitell differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT erikakunz differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT michaelhudgens differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT kennethcronin differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT kevinwiehe differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT smuniralam differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT koenkavanrompay differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT kristinadeparis differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT sallierpermar differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT manthonymoody differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques AT genevievegfouda differentadjuvantedpediatrichivenvelopevaccinesinduceddistinctplasmaantibodyresponsesdespitesimilarbcellreceptorrepertoiresininfantrhesusmacaques |