Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice
Abstract Immune cells play an important role in the development of inflammation in type 1 diabetes mellitus, so we want to explore the changes of CD4+ T cells and macrophages in vivo, which can provide an experimental basis for immunotherapy based on CD4+ T cells and macrophages. The intraperitoneal...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19031-9 |
| _version_ | 1811297360842588160 |
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| author | Chenhao Li Qingyuan Gao Hao Jiang Chengrun Liu Yujun Du Lisha Li |
| author_facet | Chenhao Li Qingyuan Gao Hao Jiang Chengrun Liu Yujun Du Lisha Li |
| author_sort | Chenhao Li |
| collection | DOAJ |
| description | Abstract Immune cells play an important role in the development of inflammation in type 1 diabetes mellitus, so we want to explore the changes of CD4+ T cells and macrophages in vivo, which can provide an experimental basis for immunotherapy based on CD4+ T cells and macrophages. The intraperitoneal injection of streptozocin was used to induce a type 1 diabetes mellitus mouse model; the blood glucose, body weight, and the expression of inflammatory factors in the kidney were measured. Immunohistochemistry was applied to determine and analyze the infiltration of CD4+ T cells and macrophages in the spleen, pancreas, and kidney. The subtypes of macrophages in the kidney and CD4+ T cells in the spleen were analyzed by flow cytometry. Our study suggests that CD4+ T cells and macrophages increase, while the inflammatory immune response system is activated in the development of T1DM. CD4+ T cells positively correlated with macrophages in the pancreas and kidney of T1DM. CD4+ T cells turn to pro-inflammatory subtypes in the spleen of T1DM, while macrophages turn to pro-inflammatory subtypes in the kidney of T1DM. Therefore, regulation of CD4+ T cells and macrophages may be a potential target for T1DM and kidney complications. |
| first_indexed | 2024-04-13T06:03:07Z |
| format | Article |
| id | doaj.art-5842bd751afb4739b77f396d35a1645f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T06:03:07Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5842bd751afb4739b77f396d35a1645f2022-12-22T02:59:21ZengNature PortfolioScientific Reports2045-23222022-09-011211910.1038/s41598-022-19031-9Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic miceChenhao Li0Qingyuan Gao1Hao Jiang2Chengrun Liu3Yujun Du4Lisha Li5Department of Nephrology, The First Hospital of Jilin UniversityThe Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin UniversityThe Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin UniversityThe Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin UniversityDepartment of Nephrology, The First Hospital of Jilin UniversityThe Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin UniversityAbstract Immune cells play an important role in the development of inflammation in type 1 diabetes mellitus, so we want to explore the changes of CD4+ T cells and macrophages in vivo, which can provide an experimental basis for immunotherapy based on CD4+ T cells and macrophages. The intraperitoneal injection of streptozocin was used to induce a type 1 diabetes mellitus mouse model; the blood glucose, body weight, and the expression of inflammatory factors in the kidney were measured. Immunohistochemistry was applied to determine and analyze the infiltration of CD4+ T cells and macrophages in the spleen, pancreas, and kidney. The subtypes of macrophages in the kidney and CD4+ T cells in the spleen were analyzed by flow cytometry. Our study suggests that CD4+ T cells and macrophages increase, while the inflammatory immune response system is activated in the development of T1DM. CD4+ T cells positively correlated with macrophages in the pancreas and kidney of T1DM. CD4+ T cells turn to pro-inflammatory subtypes in the spleen of T1DM, while macrophages turn to pro-inflammatory subtypes in the kidney of T1DM. Therefore, regulation of CD4+ T cells and macrophages may be a potential target for T1DM and kidney complications.https://doi.org/10.1038/s41598-022-19031-9 |
| spellingShingle | Chenhao Li Qingyuan Gao Hao Jiang Chengrun Liu Yujun Du Lisha Li Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice Scientific Reports |
| title | Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice |
| title_full | Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice |
| title_fullStr | Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice |
| title_full_unstemmed | Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice |
| title_short | Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice |
| title_sort | changes of macrophage and cd4 t cell in inflammatory response in type 1 diabetic mice |
| url | https://doi.org/10.1038/s41598-022-19031-9 |
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