Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq

Dental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patien...

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Main Authors: Manal B. Saleh, Hamza Sh. Abd Al-Zahra
Format: Article
Language:English
Published: University of Thi-Qar 2019-04-01
Series:مجلة علوم ذي قار
Subjects:
Online Access:http://jsci.utq.edu.iq/index.php/main/article/view/61
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author Manal B. Saleh
Hamza Sh. Abd Al-Zahra
author_facet Manal B. Saleh
Hamza Sh. Abd Al-Zahra
author_sort Manal B. Saleh
collection DOAJ
description Dental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patients with dental caries active at ages from (7-25) years from Specialized dental hospital and dental clinics at Thi-Qar province during the period from November to December 2016.Thirty-three isolates belong Streptococcus mutans were identified by cultural methods, biochemical tests and Api 20 Strep. system, while  twenty-eight  isolates identified by using Polymerase Chain Reaction (PCR) technique and sequencing of 16S rRNA gene with 507 bp, whereas by using  16S rRNA gene  the result confirmed that these isolates were belong to Streptococcus mutans. The aim of study is Isolation and identification of Streptococcus mutans bacterium from the dental caries and dental biofilm patients at Thi-Qar province by of 16S rRNA gene.
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spelling doaj.art-5842c45d118748668a55b890f101b0362023-12-05T09:58:20ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0164Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/IraqManal B. Saleh0Hamza Sh. Abd Al-Zahra 1Department of biology/ College of science/ Thi-Qar UniversityDepartment of biology/ College of science/ Thi-Qar UniversityDental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patients with dental caries active at ages from (7-25) years from Specialized dental hospital and dental clinics at Thi-Qar province during the period from November to December 2016.Thirty-three isolates belong Streptococcus mutans were identified by cultural methods, biochemical tests and Api 20 Strep. system, while  twenty-eight  isolates identified by using Polymerase Chain Reaction (PCR) technique and sequencing of 16S rRNA gene with 507 bp, whereas by using  16S rRNA gene  the result confirmed that these isolates were belong to Streptococcus mutans. The aim of study is Isolation and identification of Streptococcus mutans bacterium from the dental caries and dental biofilm patients at Thi-Qar province by of 16S rRNA gene.http://jsci.utq.edu.iq/index.php/main/article/view/61Streptococcus mutans16S rRNA genePCR
spellingShingle Manal B. Saleh
Hamza Sh. Abd Al-Zahra
Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
مجلة علوم ذي قار
Streptococcus mutans
16S rRNA gene
PCR
title Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
title_full Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
title_fullStr Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
title_full_unstemmed Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
title_short Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
title_sort isolation and identification of streptococcus mutans from dental caries patients at thi qar province iraq
topic Streptococcus mutans
16S rRNA gene
PCR
url http://jsci.utq.edu.iq/index.php/main/article/view/61
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