Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq
Dental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patien...
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Format: | Article |
Language: | English |
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University of Thi-Qar
2019-04-01
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Series: | مجلة علوم ذي قار |
Subjects: | |
Online Access: | http://jsci.utq.edu.iq/index.php/main/article/view/61 |
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author | Manal B. Saleh Hamza Sh. Abd Al-Zahra |
author_facet | Manal B. Saleh Hamza Sh. Abd Al-Zahra |
author_sort | Manal B. Saleh |
collection | DOAJ |
description | Dental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patients with dental caries active at ages from (7-25) years from Specialized dental hospital and dental clinics at Thi-Qar province during the period from November to December 2016.Thirty-three isolates belong Streptococcus mutans were identified by cultural methods, biochemical tests and Api 20 Strep. system, while twenty-eight isolates identified by using Polymerase Chain Reaction (PCR) technique and sequencing of 16S rRNA gene with 507 bp, whereas by using 16S rRNA gene the result confirmed that these isolates were belong to Streptococcus mutans. The aim of study is Isolation and identification of Streptococcus mutans bacterium from the dental caries and dental biofilm patients at Thi-Qar province by of 16S rRNA gene. |
first_indexed | 2024-03-09T02:51:08Z |
format | Article |
id | doaj.art-5842c45d118748668a55b890f101b036 |
institution | Directory Open Access Journal |
issn | 1991-8690 2709-0256 |
language | English |
last_indexed | 2024-03-09T02:51:08Z |
publishDate | 2019-04-01 |
publisher | University of Thi-Qar |
record_format | Article |
series | مجلة علوم ذي قار |
spelling | doaj.art-5842c45d118748668a55b890f101b0362023-12-05T09:58:20ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0164Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/IraqManal B. Saleh0Hamza Sh. Abd Al-Zahra 1Department of biology/ College of science/ Thi-Qar UniversityDepartment of biology/ College of science/ Thi-Qar UniversityDental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patients with dental caries active at ages from (7-25) years from Specialized dental hospital and dental clinics at Thi-Qar province during the period from November to December 2016.Thirty-three isolates belong Streptococcus mutans were identified by cultural methods, biochemical tests and Api 20 Strep. system, while twenty-eight isolates identified by using Polymerase Chain Reaction (PCR) technique and sequencing of 16S rRNA gene with 507 bp, whereas by using 16S rRNA gene the result confirmed that these isolates were belong to Streptococcus mutans. The aim of study is Isolation and identification of Streptococcus mutans bacterium from the dental caries and dental biofilm patients at Thi-Qar province by of 16S rRNA gene.http://jsci.utq.edu.iq/index.php/main/article/view/61Streptococcus mutans16S rRNA genePCR |
spellingShingle | Manal B. Saleh Hamza Sh. Abd Al-Zahra Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq مجلة علوم ذي قار Streptococcus mutans 16S rRNA gene PCR |
title | Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq |
title_full | Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq |
title_fullStr | Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq |
title_full_unstemmed | Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq |
title_short | Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq |
title_sort | isolation and identification of streptococcus mutans from dental caries patients at thi qar province iraq |
topic | Streptococcus mutans 16S rRNA gene PCR |
url | http://jsci.utq.edu.iq/index.php/main/article/view/61 |
work_keys_str_mv | AT manalbsaleh isolationandidentificationofstreptococcusmutansfromdentalcariespatientsatthiqarprovinceiraq AT hamzashabdalzahra isolationandidentificationofstreptococcusmutansfromdentalcariespatientsatthiqarprovinceiraq |