Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients
Background/Aims: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases, including autoimmune diseases. However, their role in primary biliary cholangitis (PBC) remains unclear. Here, we aimed to determine the circRNA expression profile in plasma from PBC patients and fur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | https://www.karger.com/Article/FullText/485487 |
| _version_ | 1819319652519510016 |
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| author | Jiajia Zheng Zhenrong Li Tiancheng Wang Yang Zhao Yongfeng Wang |
| author_facet | Jiajia Zheng Zhenrong Li Tiancheng Wang Yang Zhao Yongfeng Wang |
| author_sort | Jiajia Zheng |
| collection | DOAJ |
| description | Background/Aims: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases, including autoimmune diseases. However, their role in primary biliary cholangitis (PBC) remains unclear. Here, we aimed to determine the circRNA expression profile in plasma from PBC patients and further explore the value of circRNA in diagnosing PBC. Methods: CircRNA microarrays were used to determine circRNA expression profiles in plasma samples from 6 PBC patients and 6 healthy controls. Statistical analyses identified differentially expressed circRNAs, and these circRNAs were verified by qRT-PCR in 29 PBC patients and 30 healthy controls. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a map of circRNA-miRNA interactions for the differentially expressed circRNAs. Results: Our results indicated that there were 18 up-regulated and 4 down-regulated circular RNAs in the plasma from PBC patients compared with that from healthy individuals. Among the differentially expressed circRNAs, hsa_circ_402458 (P=0.0033), hsa_circ_087631 and hsa_circ_406329 (P=0.0185) were up-regulated, and hsa_circ_407176 (P=0.0066) and hsa_circ_082319 were down-regulated in the PBC group versus the healthy group as demonstrated by qRT-PCR. In particular, hsa_circ_402458 was significantly higher in PBC patients not receiving UDCA treatment than in PBC patients receiving UDCA treatment (P=0.0338). The area under the receiver operating characteristic curve for hsa_circ_402458 for diagnosing PBC was 0.710 (P=0.005). For hsa_circ_402458, two putative miRNA targets, hsa-miR-522-3p and hsa-miR-943, were predicted. Conclusions: circRNA dysregulation may play a role in PBC pathogenesis, and hsa_circ_402458 shows promise as a candidate biomarker for PBC. |
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| id | doaj.art-58435ae1c2c74a7ea07f27666903a01a |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-24T11:07:05Z |
| publishDate | 2017-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-58435ae1c2c74a7ea07f27666903a01a2022-12-21T16:58:37ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-014441271128110.1159/000485487485487Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis PatientsJiajia ZhengZhenrong LiTiancheng WangYang ZhaoYongfeng WangBackground/Aims: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases, including autoimmune diseases. However, their role in primary biliary cholangitis (PBC) remains unclear. Here, we aimed to determine the circRNA expression profile in plasma from PBC patients and further explore the value of circRNA in diagnosing PBC. Methods: CircRNA microarrays were used to determine circRNA expression profiles in plasma samples from 6 PBC patients and 6 healthy controls. Statistical analyses identified differentially expressed circRNAs, and these circRNAs were verified by qRT-PCR in 29 PBC patients and 30 healthy controls. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a map of circRNA-miRNA interactions for the differentially expressed circRNAs. Results: Our results indicated that there were 18 up-regulated and 4 down-regulated circular RNAs in the plasma from PBC patients compared with that from healthy individuals. Among the differentially expressed circRNAs, hsa_circ_402458 (P=0.0033), hsa_circ_087631 and hsa_circ_406329 (P=0.0185) were up-regulated, and hsa_circ_407176 (P=0.0066) and hsa_circ_082319 were down-regulated in the PBC group versus the healthy group as demonstrated by qRT-PCR. In particular, hsa_circ_402458 was significantly higher in PBC patients not receiving UDCA treatment than in PBC patients receiving UDCA treatment (P=0.0338). The area under the receiver operating characteristic curve for hsa_circ_402458 for diagnosing PBC was 0.710 (P=0.005). For hsa_circ_402458, two putative miRNA targets, hsa-miR-522-3p and hsa-miR-943, were predicted. Conclusions: circRNA dysregulation may play a role in PBC pathogenesis, and hsa_circ_402458 shows promise as a candidate biomarker for PBC.https://www.karger.com/Article/FullText/485487CircRNAsHsa_circ_402458PBC |
| spellingShingle | Jiajia Zheng Zhenrong Li Tiancheng Wang Yang Zhao Yongfeng Wang Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients Cellular Physiology and Biochemistry CircRNAs Hsa_circ_402458 PBC |
| title | Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients |
| title_full | Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients |
| title_fullStr | Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients |
| title_full_unstemmed | Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients |
| title_short | Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients |
| title_sort | microarray expression profile of circular rnas in plasma from primary biliary cholangitis patients |
| topic | CircRNAs Hsa_circ_402458 PBC |
| url | https://www.karger.com/Article/FullText/485487 |
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