Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype
Colorectal cancer (CRC) is the third most common cancer in the world and represents the third most deadly tumor worldwide. About 15–25% of patients present metastasis in the moment of diagnosis, the liver being the most common site of metastization. Therefore, the development of new therapeutic agen...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/1/145 |
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| author | Cristina Almeida Ana Luísa Teixeira Francisca Dias Vera Machado Mariana Morais Gabriela Martins Carlos Palmeira Maria Emília Sousa Inês Godinho Sílvia Batista Bruno Costa-Silva Rui Medeiros |
| author_facet | Cristina Almeida Ana Luísa Teixeira Francisca Dias Vera Machado Mariana Morais Gabriela Martins Carlos Palmeira Maria Emília Sousa Inês Godinho Sílvia Batista Bruno Costa-Silva Rui Medeiros |
| author_sort | Cristina Almeida |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is the third most common cancer in the world and represents the third most deadly tumor worldwide. About 15–25% of patients present metastasis in the moment of diagnosis, the liver being the most common site of metastization. Therefore, the development of new therapeutic agents is needed, to improve the patients’ prognosis. Amino acids transporters, LAT1 and ASCT2, are described as upregulated in CRC, being associated with a poor prognosis. Extracellular vesicles have emerged as key players in cell-to-cell communication due to their ability to transfer biomolecules between cells, with a phenotypic impact on the recipient cells. Thus, this study analyzes the presence of <i>LAT1</i> and <i>ASCT2</i> mRNAs in CRC-EVs and evaluates their role in phenotype modulation in a panel of four recipient cell lines (HCA-7, HEPG-2, SK-HEP-1, HKC-8). We found that HCT 116-EVs carry <i>LAT1</i>, <i>ASCT2</i> and other oncogenic mRNAs being taken up by recipient cells. Moreover, the HCT 116-EVs’ internalization was associated with the increase of <i>LAT1</i> mRNA in SK-HEP-1 cells. We also observed that HCT 116-EVs induce a higher cell migration capacity and proliferation of SK-HEP-1 and HKC-8 cells. The present study supports the <i>LAT1</i>-EVs’ mRNA involvement in cell phenotype modulation, conferring advantages in cell migration and proliferation. |
| first_indexed | 2024-03-10T01:52:52Z |
| format | Article |
| id | doaj.art-58470b97cf64477bb9c25f297703fabf |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:52:52Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-58470b97cf64477bb9c25f297703fabf2023-11-23T13:01:58ZengMDPI AGBiology2079-77372022-01-0111114510.3390/biology11010145Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive PhenotypeCristina Almeida0Ana Luísa Teixeira1Francisca Dias2Vera Machado3Mariana Morais4Gabriela Martins5Carlos Palmeira6Maria Emília Sousa7Inês Godinho8Sílvia Batista9Bruno Costa-Silva10Rui Medeiros11Molecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalMolecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalMolecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalMolecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalMolecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalImmunology Department, Portuguese Oncology Institute of Porto (IPO Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalImmunology Department, Portuguese Oncology Institute of Porto (IPO Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalImmunology Department, Portuguese Oncology Institute of Porto (IPO Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalImmunology Department, Portuguese Oncology Institute of Porto (IPO Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalSystems Oncology Group, Champalimaud Research, Champalimaud Centre for the Unknown, Av. Brasília, 1400-038 Lisbon, PortugalSystems Oncology Group, Champalimaud Research, Champalimaud Centre for the Unknown, Av. Brasília, 1400-038 Lisbon, PortugalMolecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, PortugalColorectal cancer (CRC) is the third most common cancer in the world and represents the third most deadly tumor worldwide. About 15–25% of patients present metastasis in the moment of diagnosis, the liver being the most common site of metastization. Therefore, the development of new therapeutic agents is needed, to improve the patients’ prognosis. Amino acids transporters, LAT1 and ASCT2, are described as upregulated in CRC, being associated with a poor prognosis. Extracellular vesicles have emerged as key players in cell-to-cell communication due to their ability to transfer biomolecules between cells, with a phenotypic impact on the recipient cells. Thus, this study analyzes the presence of <i>LAT1</i> and <i>ASCT2</i> mRNAs in CRC-EVs and evaluates their role in phenotype modulation in a panel of four recipient cell lines (HCA-7, HEPG-2, SK-HEP-1, HKC-8). We found that HCT 116-EVs carry <i>LAT1</i>, <i>ASCT2</i> and other oncogenic mRNAs being taken up by recipient cells. Moreover, the HCT 116-EVs’ internalization was associated with the increase of <i>LAT1</i> mRNA in SK-HEP-1 cells. We also observed that HCT 116-EVs induce a higher cell migration capacity and proliferation of SK-HEP-1 and HKC-8 cells. The present study supports the <i>LAT1</i>-EVs’ mRNA involvement in cell phenotype modulation, conferring advantages in cell migration and proliferation.https://www.mdpi.com/2079-7737/11/1/145colorectal cancer (CRC)mRNAsLAT1ASCT2extracellular vesicles (EV’s) |
| spellingShingle | Cristina Almeida Ana Luísa Teixeira Francisca Dias Vera Machado Mariana Morais Gabriela Martins Carlos Palmeira Maria Emília Sousa Inês Godinho Sílvia Batista Bruno Costa-Silva Rui Medeiros Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype Biology colorectal cancer (CRC) mRNAs LAT1 ASCT2 extracellular vesicles (EV’s) |
| title | Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype |
| title_full | Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype |
| title_fullStr | Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype |
| title_full_unstemmed | Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype |
| title_short | Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive Phenotype |
| title_sort | extracellular vesicles derived lat1 mrna as a powerful inducer of colorectal cancer aggressive phenotype |
| topic | colorectal cancer (CRC) mRNAs LAT1 ASCT2 extracellular vesicles (EV’s) |
| url | https://www.mdpi.com/2079-7737/11/1/145 |
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