Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs
This study investigated effects of dietary fumonisins (FBs) on gut and faecal microbiota of weaned pigs. In total, 18 7-week-old male pigs were fed either 0, 15 or 30 mg FBs (FB<sub>1</sub> + FB<sub>2</sub> + FB<sub>3</sub>)/kg diet for 21 days. The microbiota was...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/5/328 |
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| author | Yarsmin Yunus Zeebone Brigitta Bóta Veronika Halas Balázs Libisch Ferenc Olasz Péter Papp Tibor Keresztény Annamária Gerőcs Omeralfaroug Ali Melinda Kovács András Szabó |
| author_facet | Yarsmin Yunus Zeebone Brigitta Bóta Veronika Halas Balázs Libisch Ferenc Olasz Péter Papp Tibor Keresztény Annamária Gerőcs Omeralfaroug Ali Melinda Kovács András Szabó |
| author_sort | Yarsmin Yunus Zeebone |
| collection | DOAJ |
| description | This study investigated effects of dietary fumonisins (FBs) on gut and faecal microbiota of weaned pigs. In total, 18 7-week-old male pigs were fed either 0, 15 or 30 mg FBs (FB<sub>1</sub> + FB<sub>2</sub> + FB<sub>3</sub>)/kg diet for 21 days. The microbiota was analysed with amplicon sequencing of the 16S rRNA gene V3-V4 regions (Illumina MiSeq). Results showed no treatment effect (<i>p</i> > 0.05) on growth performance, serum reduced glutathione, glutathione peroxidase and malondialdehyde. FBs increased serum aspartate transaminase, gamma glutamyl-transferase and alkaline phosphatase activities. A 30 mg/kg FBs treatment shifted microbial population in the duodenum and ileum to lower levels (compared to control (<i>p</i> < 0.05)) of the families <i>Campylobacteraceae</i> and <i>Clostridiaceae</i>, respectively, as well as the genera <i>Alloprevotella</i>, <i>Campylobacter</i> and <i>Lachnospiraceae Incertae Sedis</i> (duodenum), <i>Turicibacter</i> (jejunum), and <i>Clostridium sensu stricto 1</i> (ileum). Faecal microbiota had higher levels of the <i>Erysipelotrichaceae</i> and <i>Ruminococcaceae</i> families and <i>Solobacterium</i>, <i>Faecalibacterium</i>, <i>Anaerofilum</i>, <i>Ruminococcus</i>, <i>Subdoligranulum</i>, <i>Pseudobutyrivibrio</i>, <i>Coprococcus</i> and <i>Roseburia</i> genera in the 30 mg/kg FBs compared to control and/or to the 15 mg/kg FBs diets. <i>Lactobacillus</i> was more abundant in the duodenum compared to faeces in all treatment groups (<i>p</i> < 0.01). Overall, the 30 mg/kg FBs diet altered the pig gut microbiota without suppressing animal growth performance. |
| first_indexed | 2024-03-11T03:15:22Z |
| format | Article |
| id | doaj.art-58481721bd8541d881c4431072f16672 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T03:15:22Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-58481721bd8541d881c4431072f166722023-11-18T03:33:30ZengMDPI AGToxins2072-66512023-05-0115532810.3390/toxins15050328Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned PigsYarsmin Yunus Zeebone0Brigitta Bóta1Veronika Halas2Balázs Libisch3Ferenc Olasz4Péter Papp5Tibor Keresztény6Annamária Gerőcs7Omeralfaroug Ali8Melinda Kovács9András Szabó10Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryELKH-MATE Mycotoxins in the Food Chain Research Group, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryDepartment of Farm Animal Nutrition, Institute of Animal Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Györgyi. Str., H-2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Györgyi. Str., H-2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Györgyi. Str., H-2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Györgyi. Str., H-2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Györgyi. Str., H-2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Guba S. Str., H-7400 Kaposvár, HungaryThis study investigated effects of dietary fumonisins (FBs) on gut and faecal microbiota of weaned pigs. In total, 18 7-week-old male pigs were fed either 0, 15 or 30 mg FBs (FB<sub>1</sub> + FB<sub>2</sub> + FB<sub>3</sub>)/kg diet for 21 days. The microbiota was analysed with amplicon sequencing of the 16S rRNA gene V3-V4 regions (Illumina MiSeq). Results showed no treatment effect (<i>p</i> > 0.05) on growth performance, serum reduced glutathione, glutathione peroxidase and malondialdehyde. FBs increased serum aspartate transaminase, gamma glutamyl-transferase and alkaline phosphatase activities. A 30 mg/kg FBs treatment shifted microbial population in the duodenum and ileum to lower levels (compared to control (<i>p</i> < 0.05)) of the families <i>Campylobacteraceae</i> and <i>Clostridiaceae</i>, respectively, as well as the genera <i>Alloprevotella</i>, <i>Campylobacter</i> and <i>Lachnospiraceae Incertae Sedis</i> (duodenum), <i>Turicibacter</i> (jejunum), and <i>Clostridium sensu stricto 1</i> (ileum). Faecal microbiota had higher levels of the <i>Erysipelotrichaceae</i> and <i>Ruminococcaceae</i> families and <i>Solobacterium</i>, <i>Faecalibacterium</i>, <i>Anaerofilum</i>, <i>Ruminococcus</i>, <i>Subdoligranulum</i>, <i>Pseudobutyrivibrio</i>, <i>Coprococcus</i> and <i>Roseburia</i> genera in the 30 mg/kg FBs compared to control and/or to the 15 mg/kg FBs diets. <i>Lactobacillus</i> was more abundant in the duodenum compared to faeces in all treatment groups (<i>p</i> < 0.01). Overall, the 30 mg/kg FBs diet altered the pig gut microbiota without suppressing animal growth performance.https://www.mdpi.com/2072-6651/15/5/328mycotoxin<i>Fusarium</i>fumonisin Bweaned pigsgut microbiotafaecal microbiota |
| spellingShingle | Yarsmin Yunus Zeebone Brigitta Bóta Veronika Halas Balázs Libisch Ferenc Olasz Péter Papp Tibor Keresztény Annamária Gerőcs Omeralfaroug Ali Melinda Kovács András Szabó Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs Toxins mycotoxin <i>Fusarium</i> fumonisin B weaned pigs gut microbiota faecal microbiota |
| title | Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs |
| title_full | Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs |
| title_fullStr | Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs |
| title_full_unstemmed | Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs |
| title_short | Gut-Faecal Microbial and Health-Marker Response to Dietary Fumonisins in Weaned Pigs |
| title_sort | gut faecal microbial and health marker response to dietary fumonisins in weaned pigs |
| topic | mycotoxin <i>Fusarium</i> fumonisin B weaned pigs gut microbiota faecal microbiota |
| url | https://www.mdpi.com/2072-6651/15/5/328 |
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