Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides
<i>Ophiopogon japonicus</i> is widely used as a tonic herb in China. According to the origins, MaiDong of Chinese materia medica can be classified as <i>Zhe MaiDong</i> (<i>Ophiopogon japonicus</i> in Zhejiang), <i>Chuan MaiDong</i> (<i>Ophiopogo...
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MDPI AG
2022-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/10/1491 |
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| author | Zherui Chen Baojie Zhu Xin Peng Shaoping Li Jing Zhao |
| author_facet | Zherui Chen Baojie Zhu Xin Peng Shaoping Li Jing Zhao |
| author_sort | Zherui Chen |
| collection | DOAJ |
| description | <i>Ophiopogon japonicus</i> is widely used as a tonic herb in China. According to the origins, MaiDong of Chinese materia medica can be classified as <i>Zhe MaiDong</i> (<i>Ophiopogon japonicus</i> in Zhejiang), <i>Chuan MaiDong</i> (<i>Ophiopogon japonicus</i> in Sichuan), <i>Duanting Shan MaiDong</i> (<i>Liriope muscari</i>), and <i>Hubei MaiDong</i> (<i>Liriope spicata</i>). In terms of quality control, polysaccharides-based evaluations have not yet been conducted. In this study, microwave-assisted extraction (MAE) was used for the preparation of polysaccharides from 29 batches of <i>MaiDong</i>. HPSEC-MALLS-RID and HPAEC-PAD were employed to investigate their molecular parameters and compositional monosaccharides, respectively. The ability to scavenge ABTS radicals and immune promotion abilities, in terms of nitric oxide releasing and phagocytosis on RAW 264.7 macrophages, were also compared. The results showed that polysaccharides in different <i>MaiDong</i> varied in molecular parameters. All polysaccharides mainly contained fructose and glucose with small amounts of arabinose, mannose, galactose, and xylose. For polysaccharides of <i>Zhe MaiDong</i> and <i>Chuan MaiDong</i>, the molar ratio of Fru to Glc was roughly 15:1 and 14:1, respectively. <i>Zhe MaiDong</i> exhibited better antioxidant and immune promotion activity, and so did that of fibrous roots. The pharmacological activity, however, did not account for the variation in growth years. Finally, indicators for quality control based on multivariate statistical analysis included: yield, antioxidant activity, the content of fructose, and RI signal. It was concluded that <i>MaiDong</i>’s fibrous roots had similar components to the root, and their quality was not significantly affected by growth age. This may provide some guidance for the cultivation and use of <i>MaiDong</i>. |
| first_indexed | 2024-03-09T20:36:53Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T20:36:53Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-584d5c0ec017479b988051e56968d6cd2023-11-23T23:09:30ZengMDPI AGBiomolecules2218-273X2022-10-011210149110.3390/biom12101491Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their PolysaccharidesZherui Chen0Baojie Zhu1Xin Peng2Shaoping Li3Jing Zhao4State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao, Macao SAR 999078, ChinaNingbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315000, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao, Macao SAR 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao, Macao SAR 999078, China<i>Ophiopogon japonicus</i> is widely used as a tonic herb in China. According to the origins, MaiDong of Chinese materia medica can be classified as <i>Zhe MaiDong</i> (<i>Ophiopogon japonicus</i> in Zhejiang), <i>Chuan MaiDong</i> (<i>Ophiopogon japonicus</i> in Sichuan), <i>Duanting Shan MaiDong</i> (<i>Liriope muscari</i>), and <i>Hubei MaiDong</i> (<i>Liriope spicata</i>). In terms of quality control, polysaccharides-based evaluations have not yet been conducted. In this study, microwave-assisted extraction (MAE) was used for the preparation of polysaccharides from 29 batches of <i>MaiDong</i>. HPSEC-MALLS-RID and HPAEC-PAD were employed to investigate their molecular parameters and compositional monosaccharides, respectively. The ability to scavenge ABTS radicals and immune promotion abilities, in terms of nitric oxide releasing and phagocytosis on RAW 264.7 macrophages, were also compared. The results showed that polysaccharides in different <i>MaiDong</i> varied in molecular parameters. All polysaccharides mainly contained fructose and glucose with small amounts of arabinose, mannose, galactose, and xylose. For polysaccharides of <i>Zhe MaiDong</i> and <i>Chuan MaiDong</i>, the molar ratio of Fru to Glc was roughly 15:1 and 14:1, respectively. <i>Zhe MaiDong</i> exhibited better antioxidant and immune promotion activity, and so did that of fibrous roots. The pharmacological activity, however, did not account for the variation in growth years. Finally, indicators for quality control based on multivariate statistical analysis included: yield, antioxidant activity, the content of fructose, and RI signal. It was concluded that <i>MaiDong</i>’s fibrous roots had similar components to the root, and their quality was not significantly affected by growth age. This may provide some guidance for the cultivation and use of <i>MaiDong</i>.https://www.mdpi.com/2218-273X/12/10/1491<i>MaiDong</i> polysaccharidesquality controlphysicochemical propertiespharmacological propertiesmultivariate statistical analysis |
| spellingShingle | Zherui Chen Baojie Zhu Xin Peng Shaoping Li Jing Zhao Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides Biomolecules <i>MaiDong</i> polysaccharides quality control physicochemical properties pharmacological properties multivariate statistical analysis |
| title | Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides |
| title_full | Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides |
| title_fullStr | Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides |
| title_full_unstemmed | Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides |
| title_short | Quality Evaluation of <i>Ophiopogon japonicus</i> from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides |
| title_sort | quality evaluation of i ophiopogon japonicus i from two authentic geographical origins in china based on physicochemical and pharmacological properties of their polysaccharides |
| topic | <i>MaiDong</i> polysaccharides quality control physicochemical properties pharmacological properties multivariate statistical analysis |
| url | https://www.mdpi.com/2218-273X/12/10/1491 |
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