3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects
Large bone defects remain an unsolved clinical challenge because of the lack of effective vascularization in newly formed bone tissue. 3D bioprinting is a fabrication technology with the potential to create vascularized bone grafts with biological activity for repairing bone defects. In this study,...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422001806 |
| _version_ | 1811235287680942080 |
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| author | Mingkui Shen Lulu Wang Yi Gao Li Feng Chuangye Xu Sijing Li Xiaohu Wang Yulan Wu Yao Guo Guoxian Pei |
| author_facet | Mingkui Shen Lulu Wang Yi Gao Li Feng Chuangye Xu Sijing Li Xiaohu Wang Yulan Wu Yao Guo Guoxian Pei |
| author_sort | Mingkui Shen |
| collection | DOAJ |
| description | Large bone defects remain an unsolved clinical challenge because of the lack of effective vascularization in newly formed bone tissue. 3D bioprinting is a fabrication technology with the potential to create vascularized bone grafts with biological activity for repairing bone defects. In this study, vascular endothelial cells laden with thermosensitive bio-ink were bioprinted in situ on the inner surfaces of interconnected tubular channels of bone mesenchymal stem cell-laden 3D-bioprinted scaffolds. Endothelial cells exhibited a more uniform distribution and greater seeding efficiency throughout the channels. In vitro, the in situ bioprinted endothelial cells can form a vascular network through proliferation and migration. The in situ vascularized tissue-engineered bone also resulted in a coupling effect between angiogenesis and osteogenesis. Moreover, RNA sequencing analysis revealed that the expression of genes related to osteogenesis and angiogenesis is upregulated in biological processes. The in vivo 3D-bioprinted in situ vascularized scaffolds exhibited excellent performance in promoting new bone formation in rat calvarial critical-sized defect models. Consequently, in situ vascularized tissue-engineered bones constructed using 3D bioprinting technology have a potential of being used as bone grafts for repairing large bone defects, with a possible clinical application in the future. |
| first_indexed | 2024-04-12T11:48:16Z |
| format | Article |
| id | doaj.art-584ed1217c3044b4a961a78d4e1b2614 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-12T11:48:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-584ed1217c3044b4a961a78d4e1b26142022-12-22T03:34:16ZengElsevierMaterials Today Bio2590-00642022-12-01161003823D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defectsMingkui Shen0Lulu Wang1Yi Gao2Li Feng3Chuangye Xu4Sijing Li5Xiaohu Wang6Yulan Wu7Yao Guo8Guoxian Pei9School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaDepartment of Orthopedics, Affiliated to Zhengzhou University, Zhengzhou, 450007, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, ChinaSchool of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China; Corresponding author.School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China; Corresponding author.Large bone defects remain an unsolved clinical challenge because of the lack of effective vascularization in newly formed bone tissue. 3D bioprinting is a fabrication technology with the potential to create vascularized bone grafts with biological activity for repairing bone defects. In this study, vascular endothelial cells laden with thermosensitive bio-ink were bioprinted in situ on the inner surfaces of interconnected tubular channels of bone mesenchymal stem cell-laden 3D-bioprinted scaffolds. Endothelial cells exhibited a more uniform distribution and greater seeding efficiency throughout the channels. In vitro, the in situ bioprinted endothelial cells can form a vascular network through proliferation and migration. The in situ vascularized tissue-engineered bone also resulted in a coupling effect between angiogenesis and osteogenesis. Moreover, RNA sequencing analysis revealed that the expression of genes related to osteogenesis and angiogenesis is upregulated in biological processes. The in vivo 3D-bioprinted in situ vascularized scaffolds exhibited excellent performance in promoting new bone formation in rat calvarial critical-sized defect models. Consequently, in situ vascularized tissue-engineered bones constructed using 3D bioprinting technology have a potential of being used as bone grafts for repairing large bone defects, with a possible clinical application in the future.http://www.sciencedirect.com/science/article/pii/S25900064220018063D bioprintingIn situ vascularizationRNA sequencing AnalysisLarge segmental bone defectsTissue engineering |
| spellingShingle | Mingkui Shen Lulu Wang Yi Gao Li Feng Chuangye Xu Sijing Li Xiaohu Wang Yulan Wu Yao Guo Guoxian Pei 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects Materials Today Bio 3D bioprinting In situ vascularization RNA sequencing Analysis Large segmental bone defects Tissue engineering |
| title | 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| title_full | 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| title_fullStr | 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| title_full_unstemmed | 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| title_short | 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| title_sort | 3d bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects |
| topic | 3D bioprinting In situ vascularization RNA sequencing Analysis Large segmental bone defects Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422001806 |
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