Involvement of Rab28 in NF-κB nuclear transport in endothelial cells.
Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic str...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3573041?pdf=render |
| _version_ | 1818922432655785984 |
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| author | Jun Jiang Ying-Xin Qi Ping Zhang Wen-Tian Gu Zhi-Qiang Yan Bao-Rong Shen Qing-Ping Yao Han Kong Shu Chien Zong-Lai Jiang |
| author_facet | Jun Jiang Ying-Xin Qi Ping Zhang Wen-Tian Gu Zhi-Qiang Yan Bao-Rong Shen Qing-Ping Yao Han Kong Shu Chien Zong-Lai Jiang |
| author_sort | Jun Jiang |
| collection | DOAJ |
| description | Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis. |
| first_indexed | 2024-12-20T01:53:27Z |
| format | Article |
| id | doaj.art-584ee704897140cc83f42f103bb2eae1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:53:27Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-584ee704897140cc83f42f103bb2eae12022-12-21T19:57:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5607610.1371/journal.pone.0056076Involvement of Rab28 in NF-κB nuclear transport in endothelial cells.Jun JiangYing-Xin QiPing ZhangWen-Tian GuZhi-Qiang YanBao-Rong ShenQing-Ping YaoHan KongShu ChienZong-Lai JiangOur previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis.http://europepmc.org/articles/PMC3573041?pdf=render |
| spellingShingle | Jun Jiang Ying-Xin Qi Ping Zhang Wen-Tian Gu Zhi-Qiang Yan Bao-Rong Shen Qing-Ping Yao Han Kong Shu Chien Zong-Lai Jiang Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. PLoS ONE |
| title | Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. |
| title_full | Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. |
| title_fullStr | Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. |
| title_full_unstemmed | Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. |
| title_short | Involvement of Rab28 in NF-κB nuclear transport in endothelial cells. |
| title_sort | involvement of rab28 in nf κb nuclear transport in endothelial cells |
| url | http://europepmc.org/articles/PMC3573041?pdf=render |
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