Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines
In cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5569 |
| _version_ | 1797499221961605120 |
|---|---|
| author | Masaya Hane Ken Kitajima Chihiro Sato |
| author_facet | Masaya Hane Ken Kitajima Chihiro Sato |
| author_sort | Masaya Hane |
| collection | DOAJ |
| description | In cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and treatment of the disease, a precise understanding of the oligo/polySia structures in cancer cells is necessary. In this study, flow cytometric analysis and gene expression datasets were obtained from sixteen different cancer cell lines. These datasets demonstrated the ability to predict glycan structures and their sialylation status. Our results also revealed that sialylation patterns are unique to each cancer cell line. Thus, we can suggest promising combinations of antibody and cancer cell for glycan prediction. However, the precise prediction of minor glycans need to be further explored. |
| first_indexed | 2024-03-10T03:44:22Z |
| format | Article |
| id | doaj.art-584fe25ed10f411e9ba2dbe435c15d07 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:22Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-584fe25ed10f411e9ba2dbe435c15d072023-11-23T11:24:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310556910.3390/ijms23105569Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell LinesMasaya Hane0Ken Kitajima1Chihiro Sato2Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, JapanBioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, JapanBioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, JapanIn cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and treatment of the disease, a precise understanding of the oligo/polySia structures in cancer cells is necessary. In this study, flow cytometric analysis and gene expression datasets were obtained from sixteen different cancer cell lines. These datasets demonstrated the ability to predict glycan structures and their sialylation status. Our results also revealed that sialylation patterns are unique to each cancer cell line. Thus, we can suggest promising combinations of antibody and cancer cell for glycan prediction. However, the precise prediction of minor glycans need to be further explored.https://www.mdpi.com/1422-0067/23/10/5569oligosialic acidpolysialic acidnon-human sialic acidgangliosidecancer |
| spellingShingle | Masaya Hane Ken Kitajima Chihiro Sato Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines International Journal of Molecular Sciences oligosialic acid polysialic acid non-human sialic acid ganglioside cancer |
| title | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
| title_full | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
| title_fullStr | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
| title_full_unstemmed | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
| title_short | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
| title_sort | comprehensive analysis of oligo polysialylglycoconjugates in cancer cell lines |
| topic | oligosialic acid polysialic acid non-human sialic acid ganglioside cancer |
| url | https://www.mdpi.com/1422-0067/23/10/5569 |
| work_keys_str_mv | AT masayahane comprehensiveanalysisofoligopolysialylglycoconjugatesincancercelllines AT kenkitajima comprehensiveanalysisofoligopolysialylglycoconjugatesincancercelllines AT chihirosato comprehensiveanalysisofoligopolysialylglycoconjugatesincancercelllines |