Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy
Polysaccharides from Panax ginseng C. A. Meyer (P. ginseng) are the main active component of P. ginseng and exhibit significant intestinal anti-inflammatory activity. However, the therapeutic mechanism of the ginseng polysaccharide is unclear, and this hinders the application for medicine or functio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.683911/full |
| _version_ | 1819105278468030464 |
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| author | Dandan Wang Dandan Wang Shuai Shao Yanqiu Zhang Daqing Zhao Daqing Zhao Mingxing Wang Mingxing Wang |
| author_facet | Dandan Wang Dandan Wang Shuai Shao Yanqiu Zhang Daqing Zhao Daqing Zhao Mingxing Wang Mingxing Wang |
| author_sort | Dandan Wang |
| collection | DOAJ |
| description | Polysaccharides from Panax ginseng C. A. Meyer (P. ginseng) are the main active component of P. ginseng and exhibit significant intestinal anti-inflammatory activity. However, the therapeutic mechanism of the ginseng polysaccharide is unclear, and this hinders the application for medicine or functional food. In this study, a polysaccharide was isolated from P. ginseng (GP). The primary structure and morphology of the GP were studied by HPLC, FT-IR spectroscopy, and scanning electron microscopy (SEM). Further, its intestinal anti-inflammatory activity and its mechanism of function were evaluated in experimental systems using DSS-induced rats, fecal microbiota transplantation (FMT), and LPS-stimulated HT-29 cells. Results showed that GP modulated the structure of gut microbiota and restored mTOR-dependent autophagic dysfunction. Consequently, active autophagy suppressed inflammation through the inhibition of NF-κB, oxidative stress, and the release of cytokines. Therefore, our research provides a rationale for future investigations into the relationship between microbiota and autophagy and revealed the therapeutic potential of GP for inflammatory bowel disease. |
| first_indexed | 2024-12-22T02:19:42Z |
| format | Article |
| id | doaj.art-5851e61b416040e08dce969eddf1a8fc |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T02:19:42Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-5851e61b416040e08dce969eddf1a8fc2022-12-21T18:42:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-07-011210.3389/fimmu.2021.683911683911Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and AutophagyDandan Wang0Dandan Wang1Shuai Shao2Yanqiu Zhang3Daqing Zhao4Daqing Zhao5Mingxing Wang6Mingxing Wang7College of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaJilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Pharmacy, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaJilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaCollege of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaJilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaPolysaccharides from Panax ginseng C. A. Meyer (P. ginseng) are the main active component of P. ginseng and exhibit significant intestinal anti-inflammatory activity. However, the therapeutic mechanism of the ginseng polysaccharide is unclear, and this hinders the application for medicine or functional food. In this study, a polysaccharide was isolated from P. ginseng (GP). The primary structure and morphology of the GP were studied by HPLC, FT-IR spectroscopy, and scanning electron microscopy (SEM). Further, its intestinal anti-inflammatory activity and its mechanism of function were evaluated in experimental systems using DSS-induced rats, fecal microbiota transplantation (FMT), and LPS-stimulated HT-29 cells. Results showed that GP modulated the structure of gut microbiota and restored mTOR-dependent autophagic dysfunction. Consequently, active autophagy suppressed inflammation through the inhibition of NF-κB, oxidative stress, and the release of cytokines. Therefore, our research provides a rationale for future investigations into the relationship between microbiota and autophagy and revealed the therapeutic potential of GP for inflammatory bowel disease.https://www.frontiersin.org/articles/10.3389/fimmu.2021.683911/fullPanax ginseng C. A. Meyerpolysaccharideintestinal inflammationgut microbiotaautophagy |
| spellingShingle | Dandan Wang Dandan Wang Shuai Shao Yanqiu Zhang Daqing Zhao Daqing Zhao Mingxing Wang Mingxing Wang Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy Frontiers in Immunology Panax ginseng C. A. Meyer polysaccharide intestinal inflammation gut microbiota autophagy |
| title | Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy |
| title_full | Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy |
| title_fullStr | Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy |
| title_full_unstemmed | Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy |
| title_short | Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy |
| title_sort | insight into polysaccharides from panax ginseng c a meyer in improving intestinal inflammation modulating intestinal microbiota and autophagy |
| topic | Panax ginseng C. A. Meyer polysaccharide intestinal inflammation gut microbiota autophagy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.683911/full |
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