Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.
Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via s...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1989-06-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520383164 |
| _version_ | 1818741370587709440 |
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| author | F R Taylor A A Kandutsch |
| author_facet | F R Taylor A A Kandutsch |
| author_sort | F R Taylor |
| collection | DOAJ |
| description | Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxylation and scission of the C20-C22 bond. Chinese hamster lung cells did not appear to derepress the reductase and these cells and Chinese hamster ovary cells did not metabolize 25-hydroxycholesterol to a significant extent. Only 5-10% of the oxysterol became esterified with a fatty acid in any of the cell lines when grown in the described culture conditions. |
| first_indexed | 2024-12-18T01:55:33Z |
| format | Article |
| id | doaj.art-58521b9993554424ae8429a482d4de6d |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:55:33Z |
| publishDate | 1989-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-58521b9993554424ae8429a482d4de6d2022-12-21T21:24:56ZengElsevierJournal of Lipid Research0022-22751989-06-01306899905Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.F R Taylor0A A Kandutsch1Jackson Laboratory, Bar Harbor, ME 04609.Jackson Laboratory, Bar Harbor, ME 04609.Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxylation and scission of the C20-C22 bond. Chinese hamster lung cells did not appear to derepress the reductase and these cells and Chinese hamster ovary cells did not metabolize 25-hydroxycholesterol to a significant extent. Only 5-10% of the oxysterol became esterified with a fatty acid in any of the cell lines when grown in the described culture conditions.http://www.sciencedirect.com/science/article/pii/S0022227520383164 |
| spellingShingle | F R Taylor A A Kandutsch Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. Journal of Lipid Research |
| title | Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. |
| title_full | Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. |
| title_fullStr | Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. |
| title_full_unstemmed | Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. |
| title_short | Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts. |
| title_sort | metabolism of 25 hydroxycholesterol in mammalian cell cultures side chain scission to pregnenolone in mouse l929 fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520383164 |
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