Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i>
<i>Bipolaris</i> species are known to be important plant pathogens that commonly cause leaf spot, root rot, and seedling blight in a wide range of hosts worldwide. In 2017, complex symptomatic cases of maydis leaf blight (caused by <i>Bipolaris maydis</i>) and maize leaf spot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/802 |
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| author | Nazia Manzar Abhijeet Shankar Kashyap Avantika Maurya Mahendra Vikram Singh Rajawat Pawan Kumar Sharma Alok Kumar Srivastava Manish Roy Anil Kumar Saxena Harsh Vardhan Singh |
| author_facet | Nazia Manzar Abhijeet Shankar Kashyap Avantika Maurya Mahendra Vikram Singh Rajawat Pawan Kumar Sharma Alok Kumar Srivastava Manish Roy Anil Kumar Saxena Harsh Vardhan Singh |
| author_sort | Nazia Manzar |
| collection | DOAJ |
| description | <i>Bipolaris</i> species are known to be important plant pathogens that commonly cause leaf spot, root rot, and seedling blight in a wide range of hosts worldwide. In 2017, complex symptomatic cases of maydis leaf blight (caused by <i>Bipolaris maydis</i>) and maize leaf spot (caused by <i>Curvularia lunata</i>) have become increasingly significant in the main maize-growing regions of India. A total of 186 samples of maydis leaf blight and 129 maize leaf spot samples were collected, in 2017, from 20 sampling sites in the main maize-growing regions of India to explore the diversity and identity of this pathogenic causal agent. A total of 77 <i>Bipolaris maydis</i> isolates and 74 <i>Curvularia lunata</i> isolates were screened based on morphological and molecular characterization and phylogenetic analysis based on ribosomal markers—nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) region, 28S nuclear ribosomal large subunit rRNA gene (LSU), D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA), and protein-coding gene-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Due to a dearth of molecular data from ex-type cultures, the use of few gene regions for species resolution, and overlapping morphological features, species recognition in <i>Bipolaris</i> has proven difficult. The present study used the multi-gene phylogenetic approach for proper identification and diversity of geographically distributed <i>B</i>. <i>maydis</i> and <i>C</i>. <i>lunata</i> isolates in Indian settings and provides useful insight into and explanation of its quantitative findings. |
| first_indexed | 2024-03-09T04:15:01Z |
| format | Article |
| id | doaj.art-5857039e01834ecc8887d2a0ad3c6668 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:15:01Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-5857039e01834ecc8887d2a0ad3c66682023-12-03T13:55:03ZengMDPI AGJournal of Fungi2309-608X2022-07-018880210.3390/jof8080802Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i>Nazia Manzar0Abhijeet Shankar Kashyap1Avantika Maurya2Mahendra Vikram Singh Rajawat3Pawan Kumar Sharma4Alok Kumar Srivastava5Manish Roy6Anil Kumar Saxena7Harsh Vardhan Singh8Plant Pathology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaMolecular Biology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaPlant Pathology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaPlant Pathology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaMicrobial Technology Unit I, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, IndiaICAR-National Bureau of Agriculturally Important Microorganisms, Maunathbhanjan 275103, India<i>Bipolaris</i> species are known to be important plant pathogens that commonly cause leaf spot, root rot, and seedling blight in a wide range of hosts worldwide. In 2017, complex symptomatic cases of maydis leaf blight (caused by <i>Bipolaris maydis</i>) and maize leaf spot (caused by <i>Curvularia lunata</i>) have become increasingly significant in the main maize-growing regions of India. A total of 186 samples of maydis leaf blight and 129 maize leaf spot samples were collected, in 2017, from 20 sampling sites in the main maize-growing regions of India to explore the diversity and identity of this pathogenic causal agent. A total of 77 <i>Bipolaris maydis</i> isolates and 74 <i>Curvularia lunata</i> isolates were screened based on morphological and molecular characterization and phylogenetic analysis based on ribosomal markers—nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) region, 28S nuclear ribosomal large subunit rRNA gene (LSU), D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA), and protein-coding gene-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Due to a dearth of molecular data from ex-type cultures, the use of few gene regions for species resolution, and overlapping morphological features, species recognition in <i>Bipolaris</i> has proven difficult. The present study used the multi-gene phylogenetic approach for proper identification and diversity of geographically distributed <i>B</i>. <i>maydis</i> and <i>C</i>. <i>lunata</i> isolates in Indian settings and provides useful insight into and explanation of its quantitative findings.https://www.mdpi.com/2309-608X/8/8/802<i>Bipolaris maydis</i><i>Curvularia lunata</i>ITSGAPDHLSUmaize |
| spellingShingle | Nazia Manzar Abhijeet Shankar Kashyap Avantika Maurya Mahendra Vikram Singh Rajawat Pawan Kumar Sharma Alok Kumar Srivastava Manish Roy Anil Kumar Saxena Harsh Vardhan Singh Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> Journal of Fungi <i>Bipolaris maydis</i> <i>Curvularia lunata</i> ITS GAPDH LSU maize |
| title | Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> |
| title_full | Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> |
| title_fullStr | Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> |
| title_full_unstemmed | Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> |
| title_short | Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i> |
| title_sort | multi gene phylogenetic approach for identification and diversity analysis of i bipolaris maydis i and i curvularia lunata i isolates causing foliar blight of i zea mays i |
| topic | <i>Bipolaris maydis</i> <i>Curvularia lunata</i> ITS GAPDH LSU maize |
| url | https://www.mdpi.com/2309-608X/8/8/802 |
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