Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro
Objectives: This study aims to investigate the effects and molecular mechanisms of apigenin (ApI) on renal ischemia/reperfusion (I/R) injury in vivo and in vitro. Methods: In vivo, the left renal artery was clamped for 45 min and the right kidney was removed to study renal I/R injury on Sprague-Dawl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-10-01
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| Series: | Renal Failure |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/0886022X.2018.1497517 |
| _version_ | 1818408711824080896 |
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| author | Xiao Wang Wei Wang Jian-Zhong Wang Cheng Yang Chao-Zhao Liang |
| author_facet | Xiao Wang Wei Wang Jian-Zhong Wang Cheng Yang Chao-Zhao Liang |
| author_sort | Xiao Wang |
| collection | DOAJ |
| description | Objectives: This study aims to investigate the effects and molecular mechanisms of apigenin (ApI) on renal ischemia/reperfusion (I/R) injury in vivo and in vitro. Methods: In vivo, the left renal artery was clamped for 45 min and the right kidney was removed to study renal I/R injury on Sprague-Dawley (SD) rats. ApI was injected at 60 min before renal ischemia. In vitro, renal tubular epithelial cells (HK-2) were pretreated with or without ApI (20 uM) for 60 min and then treated with hypoxia/reoxygenation (H/R). Renal function, histology, cells apoptosis, and cell viability were tested. Furthermore, the potential molecular mechanisms were assessed. Results: ApI pretreatment could significantly alleviated the renal function and the pathological damage as well as cells apoptosis after I/R injury. Meanwhile, ApI treatment protects H/R induced HK-2 cell apoptosis in vitro. The results of Western blot showed that ApI significantly increased the expressions of B-cell lymphoma 2 (Bcl-2) and phosphor-AKt (p-AKt), Phosphoinositide 3-kinase (PI3K), while down-regulated the expressions of Caspase3 and Bax induced by H/R injury. Conclusions: ApI pretreatment can protect renal function against I/R injury and prevent renal tubular cells from apoptosis in vivo and in vitro which might through PI3K/Akt mediated mitochondria-dependent apoptosis signaling pathway. |
| first_indexed | 2024-12-14T09:48:05Z |
| format | Article |
| id | doaj.art-58579b420c884e1f98c37c790ae82bfe |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-12-14T09:48:05Z |
| publishDate | 2018-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-58579b420c884e1f98c37c790ae82bfe2022-12-21T23:07:35ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492018-10-0140149850510.1080/0886022X.2018.14975171497517Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitroXiao Wang0Wei Wang1Jian-Zhong Wang2Cheng Yang3Chao-Zhao Liang4The First Affiliated Hospital of Anhui Medical UniversityThe First Affiliated Hospital of Anhui Medical UniversityThe First Affiliated Hospital of Anhui Medical UniversityThe First Affiliated Hospital of Anhui Medical UniversityThe First Affiliated Hospital of Anhui Medical UniversityObjectives: This study aims to investigate the effects and molecular mechanisms of apigenin (ApI) on renal ischemia/reperfusion (I/R) injury in vivo and in vitro. Methods: In vivo, the left renal artery was clamped for 45 min and the right kidney was removed to study renal I/R injury on Sprague-Dawley (SD) rats. ApI was injected at 60 min before renal ischemia. In vitro, renal tubular epithelial cells (HK-2) were pretreated with or without ApI (20 uM) for 60 min and then treated with hypoxia/reoxygenation (H/R). Renal function, histology, cells apoptosis, and cell viability were tested. Furthermore, the potential molecular mechanisms were assessed. Results: ApI pretreatment could significantly alleviated the renal function and the pathological damage as well as cells apoptosis after I/R injury. Meanwhile, ApI treatment protects H/R induced HK-2 cell apoptosis in vitro. The results of Western blot showed that ApI significantly increased the expressions of B-cell lymphoma 2 (Bcl-2) and phosphor-AKt (p-AKt), Phosphoinositide 3-kinase (PI3K), while down-regulated the expressions of Caspase3 and Bax induced by H/R injury. Conclusions: ApI pretreatment can protect renal function against I/R injury and prevent renal tubular cells from apoptosis in vivo and in vitro which might through PI3K/Akt mediated mitochondria-dependent apoptosis signaling pathway.http://dx.doi.org/10.1080/0886022X.2018.1497517Apigeninrenal ischemia/reperfusion (I/R) injuryapoptosisBcl-2AKt |
| spellingShingle | Xiao Wang Wei Wang Jian-Zhong Wang Cheng Yang Chao-Zhao Liang Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro Renal Failure Apigenin renal ischemia/reperfusion (I/R) injury apoptosis Bcl-2 AKt |
| title | Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro |
| title_full | Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro |
| title_fullStr | Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro |
| title_full_unstemmed | Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro |
| title_short | Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro |
| title_sort | effect of apigenin on apoptosis induced by renal ischemia reperfusion injury in vivo and in vitro |
| topic | Apigenin renal ischemia/reperfusion (I/R) injury apoptosis Bcl-2 AKt |
| url | http://dx.doi.org/10.1080/0886022X.2018.1497517 |
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