Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population
Abstract Background Because of the high prevalence and absence of cure for infection, chronic hepatitis B virus (HBV) infection has been acknowledged as a pressing public health issue. Toll-like receptors (TLRs) activate the human innate immune system and the polymorphisms in TLRs may alter their fu...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-018-2943-x |
| _version_ | 1831737650318409728 |
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| author | Yong Lin Zheng-Xiang Gao Xu Shen Mei-Jun Chen Yan-Ting Li Shu-Lian Li Hui-Ling Lin Qi-Feng Zhao Fan Liu Jian-Jun Niu |
| author_facet | Yong Lin Zheng-Xiang Gao Xu Shen Mei-Jun Chen Yan-Ting Li Shu-Lian Li Hui-Ling Lin Qi-Feng Zhao Fan Liu Jian-Jun Niu |
| author_sort | Yong Lin |
| collection | DOAJ |
| description | Abstract Background Because of the high prevalence and absence of cure for infection, chronic hepatitis B virus (HBV) infection has been acknowledged as a pressing public health issue. Toll-like receptors (TLRs) activate the human innate immune system and the polymorphisms in TLRs may alter their function. The present study aimed to investigate the association between TLR polymorphisms and disease progression of chronic HBV infection. Methods During the study period, 211 treatment-naïve patients with chronic HBV infection were recruited, and blood samples were collected from each individual. Matrix-assisted laser desorption/ionization time of flight mass spectrometry was employed to genotype the selected TLR polymorphisms after human genome extraction. In addition, HbsAg, TNF-α, and IL-6 levels were quantified using enzyme linked immunosorbent assay (ELISA). Statistical analyses were conducted to investigate the association between TLR polymorphisms and hepatitis activity, liver function parameters, HbsAg level, and cytokine level. Results We did not observe any mutations in rs4986790, rs4986791, and rs5743708 among all study subjects. A logistic regression revealed that mutations in rs3804099 and rs4696480 were associated with milder hepatitis activity. Consistent with the logistic regression, improved liver function parameters and reduced level of both HbsAg and cytokines were also correlated with the mutant carriers of rs3804099 and rs4696480. Conclusions TLR mutations were significantly associated with milder hepatitis activity among patients with chronic HBV infection. Therefore, we conclude that the activation of TLR pathways may further intensify the inflammation of hepatocytes, and leads to progression of disease. |
| first_indexed | 2024-12-21T13:07:38Z |
| format | Article |
| id | doaj.art-585a3d3ae7be47b78174d614f393c49c |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-21T13:07:38Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-585a3d3ae7be47b78174d614f393c49c2022-12-21T19:02:58ZengBMCBMC Infectious Diseases1471-23342018-01-011811810.1186/s12879-018-2943-xCorrelation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese populationYong Lin0Zheng-Xiang Gao1Xu Shen2Mei-Jun Chen3Yan-Ting Li4Shu-Lian Li5Hui-Ling Lin6Qi-Feng Zhao7Fan Liu8Jian-Jun Niu9Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityXiamen Huli District Maternity and Child Care HospitalXiamen Huli District Maternity and Child Care HospitalCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen UniversityAbstract Background Because of the high prevalence and absence of cure for infection, chronic hepatitis B virus (HBV) infection has been acknowledged as a pressing public health issue. Toll-like receptors (TLRs) activate the human innate immune system and the polymorphisms in TLRs may alter their function. The present study aimed to investigate the association between TLR polymorphisms and disease progression of chronic HBV infection. Methods During the study period, 211 treatment-naïve patients with chronic HBV infection were recruited, and blood samples were collected from each individual. Matrix-assisted laser desorption/ionization time of flight mass spectrometry was employed to genotype the selected TLR polymorphisms after human genome extraction. In addition, HbsAg, TNF-α, and IL-6 levels were quantified using enzyme linked immunosorbent assay (ELISA). Statistical analyses were conducted to investigate the association between TLR polymorphisms and hepatitis activity, liver function parameters, HbsAg level, and cytokine level. Results We did not observe any mutations in rs4986790, rs4986791, and rs5743708 among all study subjects. A logistic regression revealed that mutations in rs3804099 and rs4696480 were associated with milder hepatitis activity. Consistent with the logistic regression, improved liver function parameters and reduced level of both HbsAg and cytokines were also correlated with the mutant carriers of rs3804099 and rs4696480. Conclusions TLR mutations were significantly associated with milder hepatitis activity among patients with chronic HBV infection. Therefore, we conclude that the activation of TLR pathways may further intensify the inflammation of hepatocytes, and leads to progression of disease.http://link.springer.com/article/10.1186/s12879-018-2943-xToll-like receptorsHepatitis activityImmune responseHepatitis B virus |
| spellingShingle | Yong Lin Zheng-Xiang Gao Xu Shen Mei-Jun Chen Yan-Ting Li Shu-Lian Li Hui-Ling Lin Qi-Feng Zhao Fan Liu Jian-Jun Niu Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population BMC Infectious Diseases Toll-like receptors Hepatitis activity Immune response Hepatitis B virus |
| title | Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population |
| title_full | Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population |
| title_fullStr | Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population |
| title_full_unstemmed | Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population |
| title_short | Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population |
| title_sort | correlation between polymorphisms in toll like receptor genes and the activity of hepatitis b virus among treatment naive patients a case control study in a han chinese population |
| topic | Toll-like receptors Hepatitis activity Immune response Hepatitis B virus |
| url | http://link.springer.com/article/10.1186/s12879-018-2943-x |
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