In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing
Tissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(<span style="font-variant: small-caps;">d</span>,<span style=...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/11/4/180 |
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| author | Charu Dwivedi Himanshu Pandey Avinash C. Pandey Sandip Patil Pramod W. Ramteke Peter Laux Andreas Luch Ajay Vikram Singh |
| author_facet | Charu Dwivedi Himanshu Pandey Avinash C. Pandey Sandip Patil Pramod W. Ramteke Peter Laux Andreas Luch Ajay Vikram Singh |
| author_sort | Charu Dwivedi |
| collection | DOAJ |
| description | Tissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(<span style="font-variant: small-caps;">d</span>,<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA)/Gelatin polymer solutions for the simultaneous release of recombinant human epidermal growth factor (rhEGF) and gentamicin sulfate at the wound site to hasten the process of diabetic wound healing and inactivation of bacterial growth. The physicochemical characterization of the fabricated scaffolds was carried out using scanning electron microscopy (SEM) and X-ay diffraction (XRD). The scaffolds were analyzed for thermal stability using thermogravimetric analysis and differential scanning calorimetry. The porosity, biodegradability, and swelling behavior of the scaffolds was also evaluated. Encapsulation efficiency, drug loading capacity, and in vitro drug release were also investigated. Further, the bacterial inhibition percentage and detailed in vivo biocompatibility for wound healing efficiency was performed on diabetic C57BL6 mice with dorsal wounds. The scaffolds exhibited excellent wound healing and continuous proliferation of cells for 12 days. These results support the applicability of such systems in rapid healing of diabetic wounds and ulcers. |
| first_indexed | 2024-04-11T22:04:06Z |
| format | Article |
| id | doaj.art-585c842e0f2040d0981e94209e51e705 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T22:04:06Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-585c842e0f2040d0981e94209e51e7052022-12-22T04:00:48ZengMDPI AGPharmaceutics1999-49232019-04-0111418010.3390/pharmaceutics11040180pharmaceutics11040180In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound HealingCharu Dwivedi0Himanshu Pandey1Avinash C. Pandey2Sandip Patil3Pramod W. Ramteke4Peter Laux5Andreas Luch6Ajay Vikram Singh7Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 211007, IndiaNanotechnology Application Centre, Faculty of Science, University of Allahabad, Allahabad 211002, IndiaNanotechnology Application Centre, Faculty of Science, University of Allahabad, Allahabad 211002, IndiaE-Spin Nanotech Pvt Ltd., Kanpur 208016, IndiaDepartment of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 211007, IndiaDepartment of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyTissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(<span style="font-variant: small-caps;">d</span>,<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA)/Gelatin polymer solutions for the simultaneous release of recombinant human epidermal growth factor (rhEGF) and gentamicin sulfate at the wound site to hasten the process of diabetic wound healing and inactivation of bacterial growth. The physicochemical characterization of the fabricated scaffolds was carried out using scanning electron microscopy (SEM) and X-ay diffraction (XRD). The scaffolds were analyzed for thermal stability using thermogravimetric analysis and differential scanning calorimetry. The porosity, biodegradability, and swelling behavior of the scaffolds was also evaluated. Encapsulation efficiency, drug loading capacity, and in vitro drug release were also investigated. Further, the bacterial inhibition percentage and detailed in vivo biocompatibility for wound healing efficiency was performed on diabetic C57BL6 mice with dorsal wounds. The scaffolds exhibited excellent wound healing and continuous proliferation of cells for 12 days. These results support the applicability of such systems in rapid healing of diabetic wounds and ulcers.https://www.mdpi.com/1999-4923/11/4/180tissue engineeringgrowth factordiabeticwound healingnanocomposite |
| spellingShingle | Charu Dwivedi Himanshu Pandey Avinash C. Pandey Sandip Patil Pramod W. Ramteke Peter Laux Andreas Luch Ajay Vikram Singh In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing Pharmaceutics tissue engineering growth factor diabetic wound healing nanocomposite |
| title | In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing |
| title_full | In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing |
| title_fullStr | In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing |
| title_full_unstemmed | In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing |
| title_short | In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing |
| title_sort | in vivo biocompatibility of electrospun biodegradable dual carrier antibiotic growth factor in a mouse model implications for rapid wound healing |
| topic | tissue engineering growth factor diabetic wound healing nanocomposite |
| url | https://www.mdpi.com/1999-4923/11/4/180 |
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