Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis
Thioredoxins (TRXs) are small-molecule proteins with redox activity that play very important roles in the growth, development, and stress resistance of plants. Foxtail millet (Setaria italica) gradually became a model crop for stress resistance research because of its advantages such as its resistan...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.946037/full |
| _version_ | 1828111282317819904 |
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| author | Shuangxing Zhang Yang Yu Tianqi Song Mingfei Zhang Nan Li Ming Yu Hongwei Zhou Yanning Yang Sihai Guo Chunhong Xu Yongle Tu Jishan Xiang Xiaoke Zhang |
| author_facet | Shuangxing Zhang Yang Yu Tianqi Song Mingfei Zhang Nan Li Ming Yu Hongwei Zhou Yanning Yang Sihai Guo Chunhong Xu Yongle Tu Jishan Xiang Xiaoke Zhang |
| author_sort | Shuangxing Zhang |
| collection | DOAJ |
| description | Thioredoxins (TRXs) are small-molecule proteins with redox activity that play very important roles in the growth, development, and stress resistance of plants. Foxtail millet (Setaria italica) gradually became a model crop for stress resistance research because of its advantages such as its resistance to sterility and its small genome. To date, the thioredoxin (TRX) family has been identified in Arabidopsis thaliana, rice and wheat. However, studies of the TRX family in foxtail millet have not been reported, and the biological function of this family remains unclear. In this study, 35 SiTRX genes were identified in the whole genome of foxtail millet through bioinformatic analysis. According to phylogenetic analysis, 35 SiTRXs can be divided into 13 types. The chromosome distribution, gene structure, cis-elements and conserved protein motifs of 35 SiTRXs were characterized. Three nucleoredoxin (NRX) members were further identified by a structural analysis of TRX family members. The expression patterns of foxtail millet’s SiNRX members under abiotic stresses showed that they have different stress-response patterns. In addition, subcellular localization revealed that SiNRXs were localized to the nucleus, cytoplasm and membrane. Further studies demonstrated that the overexpression of SiNRX1 enhanced Arabidopsis’ tolerance to drought and salt stresses, resulting in a higher survival rate and better growth performance. Moreover, the expression levels of several known stress-related genes were generally higher in overexpressed lines than in the wild-type. Thus, this study provides a general picture of the TRX family in foxtail millet and lay a foundation for further research on the mechanism of the action of TRX proteins on abiotic stresses. |
| first_indexed | 2024-04-11T11:32:28Z |
| format | Article |
| id | doaj.art-585cfdbdcbd64c23b514d9ba923d99a0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T11:32:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-585cfdbdcbd64c23b514d9ba923d99a02022-12-22T04:26:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.946037946037Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic ArabidopsisShuangxing Zhang0Yang Yu1Tianqi Song2Mingfei Zhang3Nan Li4Ming Yu5Hongwei Zhou6Yanning Yang7Sihai Guo8Chunhong Xu9Yongle Tu10Jishan Xiang11Xiaoke Zhang12College of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaAcademy of Agricultural Sciences, Key Laboratory of Agro-Ecological Protection and Exploitation and Utilization of Animal and Plant Resources in Eastern Inner Mongolia, Chifeng University, Chifeng, ChinaAcademy of Agricultural Sciences, Key Laboratory of Agro-Ecological Protection and Exploitation and Utilization of Animal and Plant Resources in Eastern Inner Mongolia, Chifeng University, Chifeng, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaAcademy of Agricultural Sciences, Key Laboratory of Agro-Ecological Protection and Exploitation and Utilization of Animal and Plant Resources in Eastern Inner Mongolia, Chifeng University, Chifeng, ChinaCollege of Agronomy, Northwest A&F University, Xianyang, ChinaThioredoxins (TRXs) are small-molecule proteins with redox activity that play very important roles in the growth, development, and stress resistance of plants. Foxtail millet (Setaria italica) gradually became a model crop for stress resistance research because of its advantages such as its resistance to sterility and its small genome. To date, the thioredoxin (TRX) family has been identified in Arabidopsis thaliana, rice and wheat. However, studies of the TRX family in foxtail millet have not been reported, and the biological function of this family remains unclear. In this study, 35 SiTRX genes were identified in the whole genome of foxtail millet through bioinformatic analysis. According to phylogenetic analysis, 35 SiTRXs can be divided into 13 types. The chromosome distribution, gene structure, cis-elements and conserved protein motifs of 35 SiTRXs were characterized. Three nucleoredoxin (NRX) members were further identified by a structural analysis of TRX family members. The expression patterns of foxtail millet’s SiNRX members under abiotic stresses showed that they have different stress-response patterns. In addition, subcellular localization revealed that SiNRXs were localized to the nucleus, cytoplasm and membrane. Further studies demonstrated that the overexpression of SiNRX1 enhanced Arabidopsis’ tolerance to drought and salt stresses, resulting in a higher survival rate and better growth performance. Moreover, the expression levels of several known stress-related genes were generally higher in overexpressed lines than in the wild-type. Thus, this study provides a general picture of the TRX family in foxtail millet and lay a foundation for further research on the mechanism of the action of TRX proteins on abiotic stresses.https://www.frontiersin.org/articles/10.3389/fpls.2022.946037/fullfoxtail milletthioredoxinSiNRX1droughtsalt |
| spellingShingle | Shuangxing Zhang Yang Yu Tianqi Song Mingfei Zhang Nan Li Ming Yu Hongwei Zhou Yanning Yang Sihai Guo Chunhong Xu Yongle Tu Jishan Xiang Xiaoke Zhang Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis Frontiers in Plant Science foxtail millet thioredoxin SiNRX1 drought salt |
| title | Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis |
| title_full | Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis |
| title_fullStr | Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis |
| title_full_unstemmed | Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis |
| title_short | Genome-wide identification of foxtail millet’s TRX family and a functional analysis of SiNRX1 in response to drought and salt stresses in transgenic Arabidopsis |
| title_sort | genome wide identification of foxtail millet s trx family and a functional analysis of sinrx1 in response to drought and salt stresses in transgenic arabidopsis |
| topic | foxtail millet thioredoxin SiNRX1 drought salt |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.946037/full |
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