Star Polymers as Non-Viral Carriers for Apoptosis Induction
Apoptosis is a widely controlled, programmed cell death, defects in which are the source of various diseases such as neurodegenerative diseases as well as cancer. The use of apoptosis in the therapy of various human diseases is of increasing interest, and the analysis of the factors involved in its...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/5/608 |
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| author | Agnieszka Fus-Kujawa Łukasz Sieroń Estera Dobrzyńska Łukasz Chajec Barbara Mendrek Natalia Jarosz Łukasz Głowacki Kamila Dubaj Wojciech Dubaj Agnieszka Kowalczuk Karolina Bajdak-Rusinek |
| author_facet | Agnieszka Fus-Kujawa Łukasz Sieroń Estera Dobrzyńska Łukasz Chajec Barbara Mendrek Natalia Jarosz Łukasz Głowacki Kamila Dubaj Wojciech Dubaj Agnieszka Kowalczuk Karolina Bajdak-Rusinek |
| author_sort | Agnieszka Fus-Kujawa |
| collection | DOAJ |
| description | Apoptosis is a widely controlled, programmed cell death, defects in which are the source of various diseases such as neurodegenerative diseases as well as cancer. The use of apoptosis in the therapy of various human diseases is of increasing interest, and the analysis of the factors involved in its regulation is valuable in designing specific carriers capable of targeting cell death. Highly efficient and precisely controlled delivery of genetic material by low-toxic carriers is one of the most important challenges of apoptosis-based gene therapy. In this work, we investigate the effect of the star polymer with 28 poly(<i>N,N</i>′-dimethylaminoethyl methacrylate) arms (STAR) on human cells, according to its concentration and structure. We show that star polymer cytotoxicity increases within its concentration and time of cells treatment. Except for cytotoxic effect, we observe morphological changes such as a shrinkage, loss of shape and begin to detach. We also prove DNA condensation after star polymer treatment, one of the most characteristic feature of apoptosis. The results indicate that the use of STAR triggers apoptosis in cancer cells compared to various normal cells, what makes these nanoparticles a promising drug in therapeutic strategy, which targets apoptosis. We demonstrate highlighting potential of star polymers as an innovative tool for anti-cancer therapy. |
| first_indexed | 2024-03-10T03:16:28Z |
| format | Article |
| id | doaj.art-585eaa418fc64df081c7b344035cb198 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T03:16:28Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-585eaa418fc64df081c7b344035cb1982023-11-23T10:12:53ZengMDPI AGBiomolecules2218-273X2022-04-0112560810.3390/biom12050608Star Polymers as Non-Viral Carriers for Apoptosis InductionAgnieszka Fus-Kujawa0Łukasz Sieroń1Estera Dobrzyńska2Łukasz Chajec3Barbara Mendrek4Natalia Jarosz5Łukasz Głowacki6Kamila Dubaj7Wojciech Dubaj8Agnieszka Kowalczuk9Karolina Bajdak-Rusinek10Department of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandAnimal Histology and Embryology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-007 Katowice, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819 Zabrze, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819 Zabrze, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Medykow 18 Street, 40-752 Katowice, PolandApoptosis is a widely controlled, programmed cell death, defects in which are the source of various diseases such as neurodegenerative diseases as well as cancer. The use of apoptosis in the therapy of various human diseases is of increasing interest, and the analysis of the factors involved in its regulation is valuable in designing specific carriers capable of targeting cell death. Highly efficient and precisely controlled delivery of genetic material by low-toxic carriers is one of the most important challenges of apoptosis-based gene therapy. In this work, we investigate the effect of the star polymer with 28 poly(<i>N,N</i>′-dimethylaminoethyl methacrylate) arms (STAR) on human cells, according to its concentration and structure. We show that star polymer cytotoxicity increases within its concentration and time of cells treatment. Except for cytotoxic effect, we observe morphological changes such as a shrinkage, loss of shape and begin to detach. We also prove DNA condensation after star polymer treatment, one of the most characteristic feature of apoptosis. The results indicate that the use of STAR triggers apoptosis in cancer cells compared to various normal cells, what makes these nanoparticles a promising drug in therapeutic strategy, which targets apoptosis. We demonstrate highlighting potential of star polymers as an innovative tool for anti-cancer therapy.https://www.mdpi.com/2218-273X/12/5/608apoptosisstar polymers vectorsdelivery systemscross-membrane transport |
| spellingShingle | Agnieszka Fus-Kujawa Łukasz Sieroń Estera Dobrzyńska Łukasz Chajec Barbara Mendrek Natalia Jarosz Łukasz Głowacki Kamila Dubaj Wojciech Dubaj Agnieszka Kowalczuk Karolina Bajdak-Rusinek Star Polymers as Non-Viral Carriers for Apoptosis Induction Biomolecules apoptosis star polymers vectors delivery systems cross-membrane transport |
| title | Star Polymers as Non-Viral Carriers for Apoptosis Induction |
| title_full | Star Polymers as Non-Viral Carriers for Apoptosis Induction |
| title_fullStr | Star Polymers as Non-Viral Carriers for Apoptosis Induction |
| title_full_unstemmed | Star Polymers as Non-Viral Carriers for Apoptosis Induction |
| title_short | Star Polymers as Non-Viral Carriers for Apoptosis Induction |
| title_sort | star polymers as non viral carriers for apoptosis induction |
| topic | apoptosis star polymers vectors delivery systems cross-membrane transport |
| url | https://www.mdpi.com/2218-273X/12/5/608 |
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