Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue
Three-dimensional bioprinting of an endocrine pancreas is a promising future curative treatment for patients with insulin secretion deficiency. In this study, we present an end-to-end concept from the molecular to the macroscopic level. Building-blocks for a hybrid scaffold device of hydrogel and fu...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-04-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314221091033 |
| _version_ | 1811299787467653120 |
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| author | Gabriel A Salg Eric Poisel Matthias Neulinger-Munoz Jamina Gerhardus Daniel Cebulla Catrin Bludszuweit-Philipp Vitor Vieira Felix Nickel Ingrid Herr Andreas Blaeser Nathalia A Giese Thilo Hackert Hannes G Kenngott |
| author_facet | Gabriel A Salg Eric Poisel Matthias Neulinger-Munoz Jamina Gerhardus Daniel Cebulla Catrin Bludszuweit-Philipp Vitor Vieira Felix Nickel Ingrid Herr Andreas Blaeser Nathalia A Giese Thilo Hackert Hannes G Kenngott |
| author_sort | Gabriel A Salg |
| collection | DOAJ |
| description | Three-dimensional bioprinting of an endocrine pancreas is a promising future curative treatment for patients with insulin secretion deficiency. In this study, we present an end-to-end concept from the molecular to the macroscopic level. Building-blocks for a hybrid scaffold device of hydrogel and functionalized polycaprolactone were manufactured by 3D-(bio)printing. Pseudoislet formation from INS-1 cells after bioprinting resulted in a viable and proliferative experimental model. Transcriptomics showed an upregulation of proliferative and ß-cell-specific signaling cascades, downregulation of apoptotic pathways, overexpression of extracellular matrix proteins, and VEGF induced by pseudoislet formation and 3D-culture. Co-culture with endothelial cells created a natural cellular niche with enhanced insulin secretion after glucose stimulation. Survival and function of pseudoislets after explantation and extensive scaffold vascularization of both hydrogel and heparinized polycaprolactone were demonstrated in vivo . Computer simulations of oxygen, glucose and insulin flows were used to evaluate scaffold architectures and Langerhans islets at a future perivascular transplantation site. |
| first_indexed | 2024-04-13T06:42:01Z |
| format | Article |
| id | doaj.art-586238002f114a4689c233790fa2608e |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-13T06:42:01Z |
| publishDate | 2022-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-586238002f114a4689c233790fa2608e2022-12-22T02:57:43ZengSAGE PublishingJournal of Tissue Engineering2041-73142022-04-011310.1177/20417314221091033Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissueGabriel A Salg0Eric Poisel1Matthias Neulinger-Munoz2Jamina Gerhardus3Daniel Cebulla4Catrin Bludszuweit-Philipp5Vitor Vieira6Felix Nickel7Ingrid Herr8Andreas Blaeser9Nathalia A Giese10Thilo Hackert11Hannes G Kenngott12Department of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyDepartment of Dermatology and Allergy, University Hospital LMU Munich, Munich, GermanyTechnical University of Darmstadt, Institute for BioMedical Printing Technology, Darmstadt, GermanyASD Advanced Simulation and Design GmbH, Rostock, GermanyASD Advanced Simulation and Design GmbH, Rostock, GermanyINOVA DE GmbH, Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyTechnical University of Darmstadt, Institute for BioMedical Printing Technology, Darmstadt, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, GermanyThree-dimensional bioprinting of an endocrine pancreas is a promising future curative treatment for patients with insulin secretion deficiency. In this study, we present an end-to-end concept from the molecular to the macroscopic level. Building-blocks for a hybrid scaffold device of hydrogel and functionalized polycaprolactone were manufactured by 3D-(bio)printing. Pseudoislet formation from INS-1 cells after bioprinting resulted in a viable and proliferative experimental model. Transcriptomics showed an upregulation of proliferative and ß-cell-specific signaling cascades, downregulation of apoptotic pathways, overexpression of extracellular matrix proteins, and VEGF induced by pseudoislet formation and 3D-culture. Co-culture with endothelial cells created a natural cellular niche with enhanced insulin secretion after glucose stimulation. Survival and function of pseudoislets after explantation and extensive scaffold vascularization of both hydrogel and heparinized polycaprolactone were demonstrated in vivo . Computer simulations of oxygen, glucose and insulin flows were used to evaluate scaffold architectures and Langerhans islets at a future perivascular transplantation site.https://doi.org/10.1177/20417314221091033 |
| spellingShingle | Gabriel A Salg Eric Poisel Matthias Neulinger-Munoz Jamina Gerhardus Daniel Cebulla Catrin Bludszuweit-Philipp Vitor Vieira Felix Nickel Ingrid Herr Andreas Blaeser Nathalia A Giese Thilo Hackert Hannes G Kenngott Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue Journal of Tissue Engineering |
| title | Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue |
| title_full | Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue |
| title_fullStr | Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue |
| title_full_unstemmed | Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue |
| title_short | Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue |
| title_sort | toward 3d bioprinting of an endocrine pancreas a building block concept for bioartificial insulin secreting tissue |
| url | https://doi.org/10.1177/20417314221091033 |
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