Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection
Endoscopic submucosal dissection (ESD) is a surgical procedure to remove early neoplastic lesions in the gastrointestinal tract with the critical issue of perforation. A submucosal fluid cushion, such as normal saline, is used as a cushioning agent to prevent perforation; however, its cushioning mai...
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MDPI AG
2021-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/11/1696 |
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| author | Seung Jeong Han Jo Jeon Kyoung-Je Jang Sangbae Park Hyuk Soon Choi Jong Hoon Chung |
| author_facet | Seung Jeong Han Jo Jeon Kyoung-Je Jang Sangbae Park Hyuk Soon Choi Jong Hoon Chung |
| author_sort | Seung Jeong |
| collection | DOAJ |
| description | Endoscopic submucosal dissection (ESD) is a surgical procedure to remove early neoplastic lesions in the gastrointestinal tract with the critical issue of perforation. A submucosal fluid cushion, such as normal saline, is used as a cushioning agent to prevent perforation; however, its cushioning maintenance is insufficient for surgery. In this study, we introduce an injectable thermosensitive chitosan solution (CS) with <i>β</i>-glycerophosphate (<i>β</i>-GP) as a submucosal injection agent for ESD. The CS/<i>β</i>-GP system with optimal <i>β</i>-GP concentration showed drastic viscosity change near body temperature while other commercial products did not. Additionally, the injectability of the solution was similar to or greater than other commercial products. The solution with low <i>β</i>-GP concentration showed low cytotoxicity similar to other products. An in vivo preclinical study illustrated maintenance of the high cushioning of the thermosensitive solutions. These results indicate that a CS/<i>β</i>-GP system with optimal <i>β</i>-GP concentration might be used as a submucosal injection agent in ESD, and further studies are needed to validate the effectiveness of the solutions in vivo. |
| first_indexed | 2024-03-10T11:09:16Z |
| format | Article |
| id | doaj.art-5864b5215f2d4eb385d06fbe2d0741ae |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T11:09:16Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-5864b5215f2d4eb385d06fbe2d0741ae2023-11-21T20:55:53ZengMDPI AGPolymers2073-43602021-05-011311169610.3390/polym13111696Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal DissectionSeung Jeong0Han Jo Jeon1Kyoung-Je Jang2Sangbae Park3Hyuk Soon Choi4Jong Hoon Chung5Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaDivision of Agro-system Engineering, College of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaEndoscopic submucosal dissection (ESD) is a surgical procedure to remove early neoplastic lesions in the gastrointestinal tract with the critical issue of perforation. A submucosal fluid cushion, such as normal saline, is used as a cushioning agent to prevent perforation; however, its cushioning maintenance is insufficient for surgery. In this study, we introduce an injectable thermosensitive chitosan solution (CS) with <i>β</i>-glycerophosphate (<i>β</i>-GP) as a submucosal injection agent for ESD. The CS/<i>β</i>-GP system with optimal <i>β</i>-GP concentration showed drastic viscosity change near body temperature while other commercial products did not. Additionally, the injectability of the solution was similar to or greater than other commercial products. The solution with low <i>β</i>-GP concentration showed low cytotoxicity similar to other products. An in vivo preclinical study illustrated maintenance of the high cushioning of the thermosensitive solutions. These results indicate that a CS/<i>β</i>-GP system with optimal <i>β</i>-GP concentration might be used as a submucosal injection agent in ESD, and further studies are needed to validate the effectiveness of the solutions in vivo.https://www.mdpi.com/2073-4360/13/11/1696thermosensitive chitosan solution<i>β</i>-glycerophosphateendoscopic submucosal dissection (ESD)submucosal fluid cushionsubmucosal injection agent |
| spellingShingle | Seung Jeong Han Jo Jeon Kyoung-Je Jang Sangbae Park Hyuk Soon Choi Jong Hoon Chung Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection Polymers thermosensitive chitosan solution <i>β</i>-glycerophosphate endoscopic submucosal dissection (ESD) submucosal fluid cushion submucosal injection agent |
| title | Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection |
| title_full | Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection |
| title_fullStr | Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection |
| title_full_unstemmed | Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection |
| title_short | Injectable Thermosensitive Chitosan Solution with <i>β</i>-Glycerophosphate as an Optimal Submucosal Fluid Cushion for Endoscopic Submucosal Dissection |
| title_sort | injectable thermosensitive chitosan solution with i β i glycerophosphate as an optimal submucosal fluid cushion for endoscopic submucosal dissection |
| topic | thermosensitive chitosan solution <i>β</i>-glycerophosphate endoscopic submucosal dissection (ESD) submucosal fluid cushion submucosal injection agent |
| url | https://www.mdpi.com/2073-4360/13/11/1696 |
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