Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco
An obligate halophyte, Salicornia brachiata grows in salt marshes and is considered to be a potential resource of salt- and drought-responsive genes. It is important to develop an understanding of the mechanisms behind enhanced salt tolerance. To increase this understanding, a novel SbSRP gene was c...
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| Format: | Article |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00582/full |
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| author | Avinash Mishra Avinash Mishra Bhavanath Jha Bhavanath Jha Pushpika Udawat Pushpika Udawat Rajesh K. Jha Rajesh K. Jha |
| author_facet | Avinash Mishra Avinash Mishra Bhavanath Jha Bhavanath Jha Pushpika Udawat Pushpika Udawat Rajesh K. Jha Rajesh K. Jha |
| author_sort | Avinash Mishra |
| collection | DOAJ |
| description | An obligate halophyte, Salicornia brachiata grows in salt marshes and is considered to be a potential resource of salt- and drought-responsive genes. It is important to develop an understanding of the mechanisms behind enhanced salt tolerance. To increase this understanding, a novel SbSRP gene was cloned, characterized, over-expressed, and functionally validated in the model plant Nicotiana tabacum. The genome of the halophyte S. brachiata contains two homologs of an intronless SbSRP gene of 1,262 bp in length that encodes for a stress-related protein. An in vivo localization study confirmed that SbSRP is localized on the plasma membrane. Transgenic tobacco plants (T1) that constitutively over-express the SbSRP gene showed improved salinity and osmotic stress tolerance. In comparison to Wild Type (WT) and Vector Control (VC) plants, transgenic lines showed elevated relative water and chlorophyll content, lower malondialdehyde content, lower electrolyte leakage and higher accumulation of proline, free amino acids, sugars, polyphenols, and starch under abiotic stress treatments. Furthermore, a lower build-up of H2O2 content and superoxide-radicals was found in transgenic lines compared to WT and VC plants under stress conditions. Transcript expression of Nt-APX (ascorbate peroxidase), Nt-CAT (catalase), Nt-SOD (superoxide dismutase), Nt-DREB (dehydration responsive element binding factor), and Nt-AP2 (apetala2) genes was higher in transgenic lines under stress compared to WT and VC plants. The results suggested that overexpression of membrane-localized SbSRP mitigates salt and osmotic stress in the transgenic tobacco plant. It was hypothesized that SbSRP can be a transporter protein to transmit the environmental stimuli downward through the plasma membrane. However, a detailed study is required to ascertain its exact role in the abiotic stress tolerance mechanism. Overall, SbSRP is a potential candidate to be used for engineering salt and osmotic tolerance in crops. |
| first_indexed | 2024-12-11T19:53:46Z |
| format | Article |
| id | doaj.art-586620e652c948db885aac7fd4ef02a1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T19:53:46Z |
| publishDate | 2017-04-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-586620e652c948db885aac7fd4ef02a12022-12-22T00:52:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00582230803Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic TobaccoAvinash Mishra0Avinash Mishra1Bhavanath Jha2Bhavanath Jha3Pushpika Udawat4Pushpika Udawat5Rajesh K. Jha6Rajesh K. Jha7Marine Biotechnology and Ecology Division, Council of Scientific and Industrial Research-Central Salt and Marine Chemicals Research InstituteBhavnagar, IndiaAcademy of Scientific and Innovative Research, Council of Scientific and Industrial ResearchNew Delhi, IndiaMarine Biotechnology and Ecology Division, Council of Scientific and Industrial Research-Central Salt and Marine Chemicals Research InstituteBhavnagar, IndiaAcademy of Scientific and Innovative Research, Council of Scientific and Industrial ResearchNew Delhi, IndiaMarine Biotechnology and Ecology Division, Council of Scientific and Industrial Research-Central Salt and Marine Chemicals Research InstituteBhavnagar, IndiaAcademy of Scientific and Innovative Research, Council of Scientific and Industrial ResearchNew Delhi, IndiaMarine Biotechnology and Ecology Division, Council of Scientific and Industrial Research-Central Salt and Marine Chemicals Research InstituteBhavnagar, IndiaAcademy of Scientific and Innovative Research, Council of Scientific and Industrial ResearchNew Delhi, IndiaAn obligate halophyte, Salicornia brachiata grows in salt marshes and is considered to be a potential resource of salt- and drought-responsive genes. It is important to develop an understanding of the mechanisms behind enhanced salt tolerance. To increase this understanding, a novel SbSRP gene was cloned, characterized, over-expressed, and functionally validated in the model plant Nicotiana tabacum. The genome of the halophyte S. brachiata contains two homologs of an intronless SbSRP gene of 1,262 bp in length that encodes for a stress-related protein. An in vivo localization study confirmed that SbSRP is localized on the plasma membrane. Transgenic tobacco plants (T1) that constitutively over-express the SbSRP gene showed improved salinity and osmotic stress tolerance. In comparison to Wild Type (WT) and Vector Control (VC) plants, transgenic lines showed elevated relative water and chlorophyll content, lower malondialdehyde content, lower electrolyte leakage and higher accumulation of proline, free amino acids, sugars, polyphenols, and starch under abiotic stress treatments. Furthermore, a lower build-up of H2O2 content and superoxide-radicals was found in transgenic lines compared to WT and VC plants under stress conditions. Transcript expression of Nt-APX (ascorbate peroxidase), Nt-CAT (catalase), Nt-SOD (superoxide dismutase), Nt-DREB (dehydration responsive element binding factor), and Nt-AP2 (apetala2) genes was higher in transgenic lines under stress compared to WT and VC plants. The results suggested that overexpression of membrane-localized SbSRP mitigates salt and osmotic stress in the transgenic tobacco plant. It was hypothesized that SbSRP can be a transporter protein to transmit the environmental stimuli downward through the plasma membrane. However, a detailed study is required to ascertain its exact role in the abiotic stress tolerance mechanism. Overall, SbSRP is a potential candidate to be used for engineering salt and osmotic tolerance in crops.http://journal.frontiersin.org/article/10.3389/fpls.2017.00582/fullabiotic stress tolerancedroughthalophytesnovel genesalinitytransgenic |
| spellingShingle | Avinash Mishra Avinash Mishra Bhavanath Jha Bhavanath Jha Pushpika Udawat Pushpika Udawat Rajesh K. Jha Rajesh K. Jha Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco Frontiers in Plant Science abiotic stress tolerance drought halophytes novel gene salinity transgenic |
| title | Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco |
| title_full | Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco |
| title_fullStr | Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco |
| title_full_unstemmed | Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco |
| title_short | Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco |
| title_sort | overexpression of a plasma membrane localized sbsrp like protein enhances salinity and osmotic stress tolerance in transgenic tobacco |
| topic | abiotic stress tolerance drought halophytes novel gene salinity transgenic |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00582/full |
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