Association of dimethylarginines and mediators of inflammation after acute ischemic stroke
<p>Abstract</p> <p>Background</p> <p>Elevated levels of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are accompanied by endothelial dysfunction and predict adverse outcome after ischemic stroke. Via induction of oxidative stress, dimethylargi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-11-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://www.jneuroinflammation.com/content/9/1/251 |
| _version_ | 1831601493120122880 |
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| author | Chen Shufen Martens-Lobenhoffer Jens Weissenborn Karin Kielstein Jan T Lichtinghagen Ralf Deb Milani Li Na Tryc Anita B Goldbecker Annemarie Dong Qiang Bode-Böger Stefanie M Worthmann Hans |
| author_facet | Chen Shufen Martens-Lobenhoffer Jens Weissenborn Karin Kielstein Jan T Lichtinghagen Ralf Deb Milani Li Na Tryc Anita B Goldbecker Annemarie Dong Qiang Bode-Böger Stefanie M Worthmann Hans |
| author_sort | Chen Shufen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Elevated levels of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are accompanied by endothelial dysfunction and predict adverse outcome after ischemic stroke. Via induction of oxidative stress, dimethylarginines are possibly linked to the inflammatory cascade after stroke that is known to considerably contribute to secondary progression of brain injury. We sought to investigate the association between dimethylarginines and inflammatory mediators in patients with acute ischemic stroke.</p> <p>Methods</p> <p>Plasma levels of ADMA and SDMA were measured in prospectively collected blood samples of 58 patients with acute ischemic stroke. Blood samples were taken at 6 hours, 12 hours, 24 hours, 3 days and 7 days after onset of symptoms. Analyses of ADMA and SDMA were done by high-performance liquid chromatography-tandem mass spectrometry. Monocyte chemotactic protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), interleukin-6 (IL-6), C-reactive protein (CRP) and S100B as markers of inflammation and brain damage were determined by commercially available immunometric assays. Patient data were compared with control data from 32 age-adjusted healthy volunteers. Baseline stroke severity was evaluated by the National Institutes of Health Stroke Scale (NIHSS) (NIHSS 0 to 1: mild stroke; NIHSS 2 to 8: moderate stroke; NIHSS ≥9: severe stroke).</p> <p>Results</p> <p>Plasma ADMA and SDMA levels significantly correlated with blood levels of inflammatory mediators up to day 7 after stroke. On multiple stepwise linear regression analysis ADMA correlated with TIMP-1 at 6 hours, 24 hours, 3 days and 7 days, MMP-9 at 12 hours and IL-6 at 7 days (<it>P</it> <0.05) while SDMA correlated with MCP-1 at 6 hours, 24 hours, 3 days and 7 days as well as IL-6 at 3 days and 7 days (<it>P</it> <0.05).</p> <p>Conclusions</p> <p>The levels of the vasoactive compound ADMA as well as levels of its structural isomer SDMA are associated with levels of inflammatory mediators after acute ischemic stroke. Further studies need to elucidate the cause and effect relationship of these crucial players.</p> |
| first_indexed | 2024-12-18T15:03:15Z |
| format | Article |
| id | doaj.art-5868101c036143a08881b45a707884cf |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-18T15:03:15Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-5868101c036143a08881b45a707884cf2022-12-21T21:03:51ZengBMCJournal of Neuroinflammation1742-20942012-11-019125110.1186/1742-2094-9-251Association of dimethylarginines and mediators of inflammation after acute ischemic strokeChen ShufenMartens-Lobenhoffer JensWeissenborn KarinKielstein Jan TLichtinghagen RalfDeb MilaniLi NaTryc Anita BGoldbecker AnnemarieDong QiangBode-Böger Stefanie MWorthmann Hans<p>Abstract</p> <p>Background</p> <p>Elevated levels of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are accompanied by endothelial dysfunction and predict adverse outcome after ischemic stroke. Via induction of oxidative stress, dimethylarginines are possibly linked to the inflammatory cascade after stroke that is known to considerably contribute to secondary progression of brain injury. We sought to investigate the association between dimethylarginines and inflammatory mediators in patients with acute ischemic stroke.</p> <p>Methods</p> <p>Plasma levels of ADMA and SDMA were measured in prospectively collected blood samples of 58 patients with acute ischemic stroke. Blood samples were taken at 6 hours, 12 hours, 24 hours, 3 days and 7 days after onset of symptoms. Analyses of ADMA and SDMA were done by high-performance liquid chromatography-tandem mass spectrometry. Monocyte chemotactic protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), interleukin-6 (IL-6), C-reactive protein (CRP) and S100B as markers of inflammation and brain damage were determined by commercially available immunometric assays. Patient data were compared with control data from 32 age-adjusted healthy volunteers. Baseline stroke severity was evaluated by the National Institutes of Health Stroke Scale (NIHSS) (NIHSS 0 to 1: mild stroke; NIHSS 2 to 8: moderate stroke; NIHSS ≥9: severe stroke).</p> <p>Results</p> <p>Plasma ADMA and SDMA levels significantly correlated with blood levels of inflammatory mediators up to day 7 after stroke. On multiple stepwise linear regression analysis ADMA correlated with TIMP-1 at 6 hours, 24 hours, 3 days and 7 days, MMP-9 at 12 hours and IL-6 at 7 days (<it>P</it> <0.05) while SDMA correlated with MCP-1 at 6 hours, 24 hours, 3 days and 7 days as well as IL-6 at 3 days and 7 days (<it>P</it> <0.05).</p> <p>Conclusions</p> <p>The levels of the vasoactive compound ADMA as well as levels of its structural isomer SDMA are associated with levels of inflammatory mediators after acute ischemic stroke. Further studies need to elucidate the cause and effect relationship of these crucial players.</p>http://www.jneuroinflammation.com/content/9/1/251Asymmetric dimethylarginine (ADMA)Symmetric dimethylarginine (SDMA)StrokeInflammation |
| spellingShingle | Chen Shufen Martens-Lobenhoffer Jens Weissenborn Karin Kielstein Jan T Lichtinghagen Ralf Deb Milani Li Na Tryc Anita B Goldbecker Annemarie Dong Qiang Bode-Böger Stefanie M Worthmann Hans Association of dimethylarginines and mediators of inflammation after acute ischemic stroke Journal of Neuroinflammation Asymmetric dimethylarginine (ADMA) Symmetric dimethylarginine (SDMA) Stroke Inflammation |
| title | Association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| title_full | Association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| title_fullStr | Association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| title_full_unstemmed | Association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| title_short | Association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| title_sort | association of dimethylarginines and mediators of inflammation after acute ischemic stroke |
| topic | Asymmetric dimethylarginine (ADMA) Symmetric dimethylarginine (SDMA) Stroke Inflammation |
| url | http://www.jneuroinflammation.com/content/9/1/251 |
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