Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury
Glucocorticoids are widely used anti-inflammatory drugs in clinical settings. However, they can induce skeletal muscle atrophy by reducing fiber cross-sectional area and myofibrillar protein content. Studies have proven that antioxidants can improve glucocorticoid-induced skeletal muscle atrophy. Qu...
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/14/3267 |
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| author | Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Jen Chiu Hung-Che Chen Mei-Ing Chung Yu-Tse Wu Fu-An Chen |
| author_facet | Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Jen Chiu Hung-Che Chen Mei-Ing Chung Yu-Tse Wu Fu-An Chen |
| author_sort | Chun Chen |
| collection | DOAJ |
| description | Glucocorticoids are widely used anti-inflammatory drugs in clinical settings. However, they can induce skeletal muscle atrophy by reducing fiber cross-sectional area and myofibrillar protein content. Studies have proven that antioxidants can improve glucocorticoid-induced skeletal muscle atrophy. Quercetin is a potent antioxidant flavonoid widely distributed in fruits and vegetables and has shown protective effects against dexamethasone-induced skeletal muscle atrophy. In this study, we demonstrated that dexamethasone significantly inhibited cell growth and induced cell apoptosis by stimulating hydroxyl free radical production in C2C12 skeletal muscle cells. Our results evidenced that quercetin increased C2C12 skeletal cell viability and exerted antiapoptotic effects on dexamethasone-treated C2C12 cells by regulating mitochondrial membrane potential (ΔΨm) and reducing oxidative species. Quercetin can protect against dexamethasone-induced muscle atrophy by regulating the Bax/Bcl-2 ratio at the protein level and abnormal ΔΨm, which leads to the suppression of apoptosis. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:24:39Z |
| publishDate | 2020-07-01 |
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| spelling | doaj.art-5868687e692a4492b2af3f1c8b17b1502023-11-20T07:06:53ZengMDPI AGMolecules1420-30492020-07-012514326710.3390/molecules25143267Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell InjuryChun Chen0Jai-Sing Yang1Chi-Cheng Lu2Yu-Jen Chiu3Hung-Che Chen4Mei-Ing Chung5Yu-Tse Wu6Fu-An Chen7School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung City 807, TaiwanDepartment of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, TaiwanDepartment of Sport Performance, National Taiwan University of Sport, Taichung 404, TaiwanDivision of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 112, TaiwanDepartment of Pharmacy and Master Program, Tajen University, Pingtung 907, TaiwanSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung City 807, TaiwanSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung City 807, TaiwanDepartment of Pharmacy and Master Program, Tajen University, Pingtung 907, TaiwanGlucocorticoids are widely used anti-inflammatory drugs in clinical settings. However, they can induce skeletal muscle atrophy by reducing fiber cross-sectional area and myofibrillar protein content. Studies have proven that antioxidants can improve glucocorticoid-induced skeletal muscle atrophy. Quercetin is a potent antioxidant flavonoid widely distributed in fruits and vegetables and has shown protective effects against dexamethasone-induced skeletal muscle atrophy. In this study, we demonstrated that dexamethasone significantly inhibited cell growth and induced cell apoptosis by stimulating hydroxyl free radical production in C2C12 skeletal muscle cells. Our results evidenced that quercetin increased C2C12 skeletal cell viability and exerted antiapoptotic effects on dexamethasone-treated C2C12 cells by regulating mitochondrial membrane potential (ΔΨm) and reducing oxidative species. Quercetin can protect against dexamethasone-induced muscle atrophy by regulating the Bax/Bcl-2 ratio at the protein level and abnormal ΔΨm, which leads to the suppression of apoptosis.https://www.mdpi.com/1420-3049/25/14/3267apoptosisantioxidantC2C12 skeletal muscle cellsdexamethasonemitochondrial membrane potential (ΔΨm)quercetin |
| spellingShingle | Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Jen Chiu Hung-Che Chen Mei-Ing Chung Yu-Tse Wu Fu-An Chen Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury Molecules apoptosis antioxidant C2C12 skeletal muscle cells dexamethasone mitochondrial membrane potential (ΔΨm) quercetin |
| title | Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury |
| title_full | Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury |
| title_fullStr | Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury |
| title_full_unstemmed | Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury |
| title_short | Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury |
| title_sort | effect of quercetin on dexamethasone induced c2c12 skeletal muscle cell injury |
| topic | apoptosis antioxidant C2C12 skeletal muscle cells dexamethasone mitochondrial membrane potential (ΔΨm) quercetin |
| url | https://www.mdpi.com/1420-3049/25/14/3267 |
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