Cryopreservation of Pistacia vera embryonic axes
This preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually de...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-04-01
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| Series: | Journal of Forest Science |
| Subjects: | |
| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-201504-0006_cryopreservation-of-pistacia-vera-embryonic-axes.php |
| _version_ | 1828008010005348352 |
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| author | B. Benmahioul F. Daguin M. Kaïd-Harche |
| author_facet | B. Benmahioul F. Daguin M. Kaïd-Harche |
| author_sort | B. Benmahioul |
| collection | DOAJ |
| description | This preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually decreased from 100% to 31% after 30-month storage of seeds. Cryopreservation may thus be necessary for the long-term conservation of embryos. A simple protocol was set up using embryonic axes. It included a single dehydration step with silica gel prior to direct freezing in liquid nitrogen (-196°C). The optimal germination rate was obtained after 60 min dehydration (water content of 0.2 grams of water per gram of dry weight [g.g-1 DW]). However, 90 minutes of dehydration (0.14 g.g-1 DW) were necessary to obtain seedlings whose qualitative development was equivalent to that of the control embryonic axes. |
| first_indexed | 2024-04-10T08:18:23Z |
| format | Article |
| id | doaj.art-586d426f3e9841c493eb381f3d10fdc6 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-10T08:18:23Z |
| publishDate | 2015-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-586d426f3e9841c493eb381f3d10fdc62023-02-23T03:42:38ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2015-04-0161418218710.17221/63/2014-JFSjfs-201504-0006Cryopreservation of Pistacia vera embryonic axesB. Benmahioul0F. Daguin1M. Kaïd-Harche2Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Tlemcen, AlgeriaUMR 1345 Research Institute of Horticulture and Seeds, University of Angers, France Angers, FranceDepartment of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, AlgeriaThis preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually decreased from 100% to 31% after 30-month storage of seeds. Cryopreservation may thus be necessary for the long-term conservation of embryos. A simple protocol was set up using embryonic axes. It included a single dehydration step with silica gel prior to direct freezing in liquid nitrogen (-196°C). The optimal germination rate was obtained after 60 min dehydration (water content of 0.2 grams of water per gram of dry weight [g.g-1 DW]). However, 90 minutes of dehydration (0.14 g.g-1 DW) were necessary to obtain seedlings whose qualitative development was equivalent to that of the control embryonic axes.https://jfs.agriculturejournals.cz/artkey/jfs-201504-0006_cryopreservation-of-pistacia-vera-embryonic-axes.phpseeddehydrationwater contentliquid nitrogengermination |
| spellingShingle | B. Benmahioul F. Daguin M. Kaïd-Harche Cryopreservation of Pistacia vera embryonic axes Journal of Forest Science seed dehydration water content liquid nitrogen germination |
| title | Cryopreservation of Pistacia vera embryonic axes |
| title_full | Cryopreservation of Pistacia vera embryonic axes |
| title_fullStr | Cryopreservation of Pistacia vera embryonic axes |
| title_full_unstemmed | Cryopreservation of Pistacia vera embryonic axes |
| title_short | Cryopreservation of Pistacia vera embryonic axes |
| title_sort | cryopreservation of pistacia vera embryonic axes |
| topic | seed dehydration water content liquid nitrogen germination |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-201504-0006_cryopreservation-of-pistacia-vera-embryonic-axes.php |
| work_keys_str_mv | AT bbenmahioul cryopreservationofpistaciaveraembryonicaxes AT fdaguin cryopreservationofpistaciaveraembryonicaxes AT mkaidharche cryopreservationofpistaciaveraembryonicaxes |