Polymorphism of growth hormone gene in three goat breeds in Egypt
Abstract Background In Egypt, like other developing countries, goats are prime resources of meat. So, selection of goats for superior growth rate is advantageous. Growth hormone (GH) is the main regulator of animal growth, and encoded by GH gene that exhibits active gene variants improving growth. T...
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| Language: | English |
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SpringerOpen
2018-12-01
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| Series: | Bulletin of the National Research Centre |
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| Online Access: | http://link.springer.com/article/10.1186/s42269-018-0035-0 |
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| author | Karima Fathy Mahrous Sekena H. Abdel-Aziem Mohamed A. M. Abdel-Hafez Mohamed Abdel-Mordy Hossam E. Rushdi |
| author_facet | Karima Fathy Mahrous Sekena H. Abdel-Aziem Mohamed A. M. Abdel-Hafez Mohamed Abdel-Mordy Hossam E. Rushdi |
| author_sort | Karima Fathy Mahrous |
| collection | DOAJ |
| description | Abstract Background In Egypt, like other developing countries, goats are prime resources of meat. So, selection of goats for superior growth rate is advantageous. Growth hormone (GH) is the main regulator of animal growth, and encoded by GH gene that exhibits active gene variants improving growth. The objective of this study was to identify GH gene variants in three goat breeds (Barki, Damascus, and Zaraibi), via polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and gene sequencing analyses. Results Three loci on GH gene named GH1, GH2, and GH6 polymorphisms were analyzed. GH1-HaeIII/RFLP showed only two genotypes (AB and BB) in all breeds, with absent AA genotype. Both Barki and Zaraibi exhibited the highest GC genotype frequency (0.95). GH2-HaeIII/RFLP produced only two homozygous genotypes AA in Damascus and BB in both Barki and Zaraibi, with the absence of AB genotype in the three breeds. However, digestion of GH6 by HaeIII was monomorphic; it exhibited different single-nucleotide polymorphisms (SNPs), detected by DNA sequencing, among the studied goat breeds. Conclusions The revealed SNPs could be employed as useful markers, helping goat breeders in selection of goats for high growth performance. Further analyses with larger sample size are needed for investigating the relationship between the different genotypes and growth traits. |
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| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-12T06:21:54Z |
| publishDate | 2018-12-01 |
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| series | Bulletin of the National Research Centre |
| spelling | doaj.art-5871a9aeda4c432a8c1ded630a3e92632022-12-22T00:34:53ZengSpringerOpenBulletin of the National Research Centre2522-83072018-12-014211710.1186/s42269-018-0035-0Polymorphism of growth hormone gene in three goat breeds in EgyptKarima Fathy Mahrous0Sekena H. Abdel-Aziem1Mohamed A. M. Abdel-Hafez2Mohamed Abdel-Mordy3Hossam E. Rushdi4Genetic Engineering and Biotechnology Research Division, National Research CentreDepartment of Cell Biology, National Research CentreDepartment of Cell Biology, National Research CentreDepartment of Zoology, Faculty of Science, Ain Shams UniversityDepartment of Animal Production, Faculty of Agriculture, Cairo University GizaAbstract Background In Egypt, like other developing countries, goats are prime resources of meat. So, selection of goats for superior growth rate is advantageous. Growth hormone (GH) is the main regulator of animal growth, and encoded by GH gene that exhibits active gene variants improving growth. The objective of this study was to identify GH gene variants in three goat breeds (Barki, Damascus, and Zaraibi), via polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and gene sequencing analyses. Results Three loci on GH gene named GH1, GH2, and GH6 polymorphisms were analyzed. GH1-HaeIII/RFLP showed only two genotypes (AB and BB) in all breeds, with absent AA genotype. Both Barki and Zaraibi exhibited the highest GC genotype frequency (0.95). GH2-HaeIII/RFLP produced only two homozygous genotypes AA in Damascus and BB in both Barki and Zaraibi, with the absence of AB genotype in the three breeds. However, digestion of GH6 by HaeIII was monomorphic; it exhibited different single-nucleotide polymorphisms (SNPs), detected by DNA sequencing, among the studied goat breeds. Conclusions The revealed SNPs could be employed as useful markers, helping goat breeders in selection of goats for high growth performance. Further analyses with larger sample size are needed for investigating the relationship between the different genotypes and growth traits.http://link.springer.com/article/10.1186/s42269-018-0035-0GoatPolymorphismPCR-RFLPSNPGrowth hormone gene |
| spellingShingle | Karima Fathy Mahrous Sekena H. Abdel-Aziem Mohamed A. M. Abdel-Hafez Mohamed Abdel-Mordy Hossam E. Rushdi Polymorphism of growth hormone gene in three goat breeds in Egypt Bulletin of the National Research Centre Goat Polymorphism PCR-RFLP SNP Growth hormone gene |
| title | Polymorphism of growth hormone gene in three goat breeds in Egypt |
| title_full | Polymorphism of growth hormone gene in three goat breeds in Egypt |
| title_fullStr | Polymorphism of growth hormone gene in three goat breeds in Egypt |
| title_full_unstemmed | Polymorphism of growth hormone gene in three goat breeds in Egypt |
| title_short | Polymorphism of growth hormone gene in three goat breeds in Egypt |
| title_sort | polymorphism of growth hormone gene in three goat breeds in egypt |
| topic | Goat Polymorphism PCR-RFLP SNP Growth hormone gene |
| url | http://link.springer.com/article/10.1186/s42269-018-0035-0 |
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