The effects of tadalafil on renal ischemia reperfusion injury: an experimental study
Many pharmacological agents were investigated for the prevention of renal ischemic reperfusion (I/R) injury as well as the phosphodiesterase (PDE) inhibitors. The aim of the study was to examine the possible renoprotective effect of a member in this family, tadalafil (Td) on I/R injury. Thirty-six S...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2011-08-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2567 |
| _version_ | 1797260882647973888 |
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| author | Feyzul Gasanov Berna Aytac Hakan Vuruskan |
| author_facet | Feyzul Gasanov Berna Aytac Hakan Vuruskan |
| author_sort | Feyzul Gasanov |
| collection | DOAJ |
| description | Many pharmacological agents were investigated for the prevention of renal ischemic reperfusion (I/R) injury as well as the phosphodiesterase (PDE) inhibitors. The aim of the study was to examine the possible renoprotective effect of a member in this family, tadalafil (Td) on I/R injury. Thirty-six Spraque Dawley rats were allocated to six groups as; control, sham, ischemia (I), ischemia/reperfusion (I/R), Td pretreatment ischemia (Td/I) and Td pretreatment ischemia/reperfusion (Td/IR) groups. Right nephrectomy was performed in all groups. Td was dissolved in saline solution and given as a single dose (1mg/kg) through an orogastrictube 60 min before the operation in the Td pretreatment groups. In ischemia group the left renal pedicle was occluded for 45 minutes and after than underwent left nephrectomy. In I/R group left renal pedicle was occluded for 45 minutes, reperfused for 1 hour and after then underwent nephrectomy. The left kidneys were evaluated after standard laboratory procedures with regard to tubular morphology, and leukocyte infiltration. The data were analyzed by using Kruskal–Wallis test to determine differences among the groups. A p value of < 0.05 was considered significant.
Renal tubular damage was significant increased in the ischemia and I/R group (Groups III and IV) when compared to those in the sham group (Group II), (p = 0.004, 0.004, respectively). Tubular damage, in the Td pretreatment ischemia (Td/I) (Group V) and Td pretreatment ischemia/reperfusion (Td/IR) (Group VI) were less than that in the ischemia group (Group III) (p= 0.010, p= 0.025, respectively).
Td administration prior to the renal I/R injury attenuated these morphological disarrangements, which were observed in renal I/R. Tubular necrosis, which may be considered as an important issue of the developing renal injury, was also completely prevented with Td administration.
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| first_indexed | 2024-04-24T23:32:23Z |
| format | Article |
| id | doaj.art-587683f22c0e44f3be23b4885bb935d6 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:23Z |
| publishDate | 2011-08-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-587683f22c0e44f3be23b4885bb935d62024-03-15T14:33:40ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2011-08-0111310.17305/bjbms.2011.2567334The effects of tadalafil on renal ischemia reperfusion injury: an experimental studyFeyzul Gasanov0Berna Aytac1Hakan Vuruskan2Department of Urology, Faculty of Medicine, Uludag UniversityDepartment of Surgical Pathology, Faculty of Medicine, Uludag UniversityDepartment of Urology, Faculty of Medicine, Uludag UniversityMany pharmacological agents were investigated for the prevention of renal ischemic reperfusion (I/R) injury as well as the phosphodiesterase (PDE) inhibitors. The aim of the study was to examine the possible renoprotective effect of a member in this family, tadalafil (Td) on I/R injury. Thirty-six Spraque Dawley rats were allocated to six groups as; control, sham, ischemia (I), ischemia/reperfusion (I/R), Td pretreatment ischemia (Td/I) and Td pretreatment ischemia/reperfusion (Td/IR) groups. Right nephrectomy was performed in all groups. Td was dissolved in saline solution and given as a single dose (1mg/kg) through an orogastrictube 60 min before the operation in the Td pretreatment groups. In ischemia group the left renal pedicle was occluded for 45 minutes and after than underwent left nephrectomy. In I/R group left renal pedicle was occluded for 45 minutes, reperfused for 1 hour and after then underwent nephrectomy. The left kidneys were evaluated after standard laboratory procedures with regard to tubular morphology, and leukocyte infiltration. The data were analyzed by using Kruskal–Wallis test to determine differences among the groups. A p value of < 0.05 was considered significant. Renal tubular damage was significant increased in the ischemia and I/R group (Groups III and IV) when compared to those in the sham group (Group II), (p = 0.004, 0.004, respectively). Tubular damage, in the Td pretreatment ischemia (Td/I) (Group V) and Td pretreatment ischemia/reperfusion (Td/IR) (Group VI) were less than that in the ischemia group (Group III) (p= 0.010, p= 0.025, respectively). Td administration prior to the renal I/R injury attenuated these morphological disarrangements, which were observed in renal I/R. Tubular necrosis, which may be considered as an important issue of the developing renal injury, was also completely prevented with Td administration. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2567kidneyischemia/reperfusion injurytadalafilrat |
| spellingShingle | Feyzul Gasanov Berna Aytac Hakan Vuruskan The effects of tadalafil on renal ischemia reperfusion injury: an experimental study Biomolecules & Biomedicine kidney ischemia/reperfusion injury tadalafil rat |
| title | The effects of tadalafil on renal ischemia reperfusion injury: an experimental study |
| title_full | The effects of tadalafil on renal ischemia reperfusion injury: an experimental study |
| title_fullStr | The effects of tadalafil on renal ischemia reperfusion injury: an experimental study |
| title_full_unstemmed | The effects of tadalafil on renal ischemia reperfusion injury: an experimental study |
| title_short | The effects of tadalafil on renal ischemia reperfusion injury: an experimental study |
| title_sort | effects of tadalafil on renal ischemia reperfusion injury an experimental study |
| topic | kidney ischemia/reperfusion injury tadalafil rat |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2567 |
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