CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela
<p>Abstract</p> <p>A clinical isolate of <it>C. freundii </it>with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-05-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | http://www.ann-clinmicrob.com/content/10/1/22 |
| _version_ | 1818641430856335360 |
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| author | Gutkind Gabriel Díaz Tulia Ghiglione Bárbara Millán Beatriz Araque María |
| author_facet | Gutkind Gabriel Díaz Tulia Ghiglione Bárbara Millán Beatriz Araque María |
| author_sort | Gutkind Gabriel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>A clinical isolate of <it>C. freundii </it>with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the <it>bla</it><sub>CTX-M-14 </sub>gene in the pLM07/20 from <it>C. freundii</it>.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.</p> |
| first_indexed | 2024-12-16T23:27:03Z |
| format | Article |
| id | doaj.art-587e85d826274bd2afa205fd37cc4a67 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-16T23:27:03Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-587e85d826274bd2afa205fd37cc4a672022-12-21T22:11:59ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112011-05-011012210.1186/1476-0711-10-22CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in VenezuelaGutkind GabrielDíaz TuliaGhiglione BárbaraMillán BeatrizAraque María<p>Abstract</p> <p>A clinical isolate of <it>C. freundii </it>with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the <it>bla</it><sub>CTX-M-14 </sub>gene in the pLM07/20 from <it>C. freundii</it>.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.</p>http://www.ann-clinmicrob.com/content/10/1/22<it>Citrobacter freundii</it>Extended-spectrum β-lactamasesCTX-M-14, plasmidFrepB replicon |
| spellingShingle | Gutkind Gabriel Díaz Tulia Ghiglione Bárbara Millán Beatriz Araque María CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela Annals of Clinical Microbiology and Antimicrobials <it>Citrobacter freundii</it> Extended-spectrum β-lactamases CTX-M-14, plasmid FrepB replicon |
| title | CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela |
| title_full | CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela |
| title_fullStr | CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela |
| title_full_unstemmed | CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela |
| title_short | CTX-M-14 β-lactamase-producing <it>Citrobacter freundii </it>isolated in Venezuela |
| title_sort | ctx m 14 β lactamase producing it citrobacter freundii it isolated in venezuela |
| topic | <it>Citrobacter freundii</it> Extended-spectrum β-lactamases CTX-M-14, plasmid FrepB replicon |
| url | http://www.ann-clinmicrob.com/content/10/1/22 |
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