Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri
Harmful algae blooms have expanded greatly in recent decades, and their secreted toxins pose a severe threat to human health and marine ecosystems. Saxitoxin (STX) is a main paralytic shellfish poison naturally produced by marine microalgae of the genus Alexandrium. Despite numerous studies have ass...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01173/full |
| _version_ | 1818822786142961664 |
|---|---|
| author | Ruiwen Cao Ruiwen Cao Ruiwen Cao Dan Wang Qianyu Wei Qing Wang Qing Wang Dinglong Yang Dinglong Yang Hui Liu Hui Liu Zhijun Dong Zhijun Dong Xiaoli Zhang Xiaoli Zhang Qianqian Zhang Qianqian Zhang Jianmin Zhao Jianmin Zhao |
| author_facet | Ruiwen Cao Ruiwen Cao Ruiwen Cao Dan Wang Qianyu Wei Qing Wang Qing Wang Dinglong Yang Dinglong Yang Hui Liu Hui Liu Zhijun Dong Zhijun Dong Xiaoli Zhang Xiaoli Zhang Qianqian Zhang Qianqian Zhang Jianmin Zhao Jianmin Zhao |
| author_sort | Ruiwen Cao |
| collection | DOAJ |
| description | Harmful algae blooms have expanded greatly in recent decades, and their secreted toxins pose a severe threat to human health and marine ecosystems. Saxitoxin (STX) is a main paralytic shellfish poison naturally produced by marine microalgae of the genus Alexandrium. Despite numerous studies have assessed the impacts of STX on marine bivalves, comparative in vivo study on the toxicity of STX on bivalves with distinct accumulation ability (such as oysters and scallops) has been seldom investigated. The aim of this study was to identify whether distinct sensitivity exists between oysters, Crassostrea gigas, and scallops, Chlamys farreri under the same amount of STX exposure using multiple biomarker responses. The responses of different biochemical markers including oxidative stress markers (catalase, superoxide dismutase, glutathione S-transferase, and lipid peroxidation) and immunotoxicity biomarkers (hemocyte phagocytosis rate, reactive oxidative species production, and DNA damages) were evaluated in bivalves after 12, 48, and 96 h of exposure to STX. The integrated biomarker responses value combined with two-way ANOVA analysis suggested that STX posed slightly severer stress on scallops than oysters for the extended period of time. This study provided preliminary results on the usefulness of a multi-biomarker approach to assess the toxicity associated with STX exposure in marine bivalves. |
| first_indexed | 2024-12-18T23:29:37Z |
| format | Article |
| id | doaj.art-587fb1079a7145d89c4f773477a81e08 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-18T23:29:37Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-587fb1079a7145d89c4f773477a81e082022-12-21T20:47:43ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-08-01910.3389/fphys.2018.01173409277Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreriRuiwen Cao0Ruiwen Cao1Ruiwen Cao2Dan Wang3Qianyu Wei4Qing Wang5Qing Wang6Dinglong Yang7Dinglong Yang8Hui Liu9Hui Liu10Zhijun Dong11Zhijun Dong12Xiaoli Zhang13Xiaoli Zhang14Qianqian Zhang15Qianqian Zhang16Jianmin Zhao17Jianmin Zhao18Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaMuping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, ChinaHarmful algae blooms have expanded greatly in recent decades, and their secreted toxins pose a severe threat to human health and marine ecosystems. Saxitoxin (STX) is a main paralytic shellfish poison naturally produced by marine microalgae of the genus Alexandrium. Despite numerous studies have assessed the impacts of STX on marine bivalves, comparative in vivo study on the toxicity of STX on bivalves with distinct accumulation ability (such as oysters and scallops) has been seldom investigated. The aim of this study was to identify whether distinct sensitivity exists between oysters, Crassostrea gigas, and scallops, Chlamys farreri under the same amount of STX exposure using multiple biomarker responses. The responses of different biochemical markers including oxidative stress markers (catalase, superoxide dismutase, glutathione S-transferase, and lipid peroxidation) and immunotoxicity biomarkers (hemocyte phagocytosis rate, reactive oxidative species production, and DNA damages) were evaluated in bivalves after 12, 48, and 96 h of exposure to STX. The integrated biomarker responses value combined with two-way ANOVA analysis suggested that STX posed slightly severer stress on scallops than oysters for the extended period of time. This study provided preliminary results on the usefulness of a multi-biomarker approach to assess the toxicity associated with STX exposure in marine bivalves.https://www.frontiersin.org/article/10.3389/fphys.2018.01173/fullsaxitoxinCrassostrea gigasChlamys farrerioxidative stressimmunotoxicity |
| spellingShingle | Ruiwen Cao Ruiwen Cao Ruiwen Cao Dan Wang Qianyu Wei Qing Wang Qing Wang Dinglong Yang Dinglong Yang Hui Liu Hui Liu Zhijun Dong Zhijun Dong Xiaoli Zhang Xiaoli Zhang Qianqian Zhang Qianqian Zhang Jianmin Zhao Jianmin Zhao Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri Frontiers in Physiology saxitoxin Crassostrea gigas Chlamys farreri oxidative stress immunotoxicity |
| title | Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri |
| title_full | Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri |
| title_fullStr | Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri |
| title_full_unstemmed | Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri |
| title_short | Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri |
| title_sort | integrative biomarker assessment of the influence of saxitoxin on marine bivalves a comparative study of the two bivalve species oysters crassostrea gigas and scallops chlamys farreri |
| topic | saxitoxin Crassostrea gigas Chlamys farreri oxidative stress immunotoxicity |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01173/full |
| work_keys_str_mv | AT ruiwencao integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT ruiwencao integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT ruiwencao integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT danwang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT qianyuwei integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT qingwang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT qingwang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT dinglongyang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT dinglongyang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT huiliu integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT huiliu integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT zhijundong integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT zhijundong integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT xiaolizhang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT xiaolizhang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT qianqianzhang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT qianqianzhang integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT jianminzhao integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri AT jianminzhao integrativebiomarkerassessmentoftheinfluenceofsaxitoxinonmarinebivalvesacomparativestudyofthetwobivalvespeciesoysterscrassostreagigasandscallopschlamysfarreri |