Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis
Xiaoqing Gang, Mengmeng Yuan, Juxin Zhang Department of Gynecology, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, Zhengzhou 450003, Henan, People’s Republic of ChinaCorrespondence: Juxin ZhangDepartment of Gynecology, Henan Provincial&n...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-03-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cervical-cancer-cell-proliferati-peer-reviewed-article-CMAR |
| _version_ | 1818386854611779584 |
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| author | Gang X Yuan M Zhang J |
| author_facet | Gang X Yuan M Zhang J |
| author_sort | Gang X |
| collection | DOAJ |
| description | Xiaoqing Gang, Mengmeng Yuan, Juxin Zhang Department of Gynecology, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, Zhengzhou 450003, Henan, People’s Republic of ChinaCorrespondence: Juxin ZhangDepartment of Gynecology, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, No. 7 Weiwu Road, Jinshui District, Zhengzhou, Henan 450003, People’s Republic of ChinaTel +86-138 3818 4667Email zjuxin667@163.comObjective: Long non-coding RNAs (lncRNAs) have been identified as important players in tumorigenesis. LncRNA TMPO antisense RNA 1 (TMPO-AS1) has been shown to be involved in several tumors. However, the functional role and the underlying mechanism of TMPO-AS1 in regulating cervical cancer cell behavior remain unclear.Materials and Methods: Expression of TMPO-AS1, miR-143-3p, and ZEB1 were examined by qRT-PCR and Western blot. Cell proliferation, migration and invasion were evaluated using CCK-8 assay and Transwell migration and invasion assays, respectively. Luciferase reporter assay was performed to investigate the interaction miR-143-3p and TMPO-AS1 or ZEB1.Results: TMPO-AS1 was highly expressed in cervical cancer cells. Furthermore, TMPO-AS1 overexpression significantly promoted C-33A cell proliferation, migration, and invasion. In contrast, TMPO-AS1 silencing inhibited SiHa cell proliferation, migration, and invasion. Mechanistically, TMPO-AS1 acted as a sponge of miR-143-3p to elevate expression of zinc finger E-box binding homeobox 1 (ZEB1), a target of miR-143-3p, and thereby promoted C-33A cell proliferation, migration, and invasion. Further assays showed that TMPO-AS1 knockdown inhibited cervical cancer cell tumorigenesis in vivo.Conclusion: TMPO-AS1 promotes cervical cancer cell proliferation, migration, and invasion by regulating the miR-143-3p/ZEB1 axis.Keywords: cervical cancer, TMPO-AS1, miR-143-3p, ZEB1 |
| first_indexed | 2024-12-14T04:00:40Z |
| format | Article |
| id | doaj.art-5883fcb273ec4fe297dbcf53d51eca71 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-14T04:00:40Z |
| publishDate | 2020-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-5883fcb273ec4fe297dbcf53d51eca712022-12-21T23:17:58ZengDove Medical PressCancer Management and Research1179-13222020-03-01Volume 121587159952234Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 AxisGang XYuan MZhang JXiaoqing Gang, Mengmeng Yuan, Juxin Zhang Department of Gynecology, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, Zhengzhou 450003, Henan, People’s Republic of ChinaCorrespondence: Juxin ZhangDepartment of Gynecology, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, No. 7 Weiwu Road, Jinshui District, Zhengzhou, Henan 450003, People’s Republic of ChinaTel +86-138 3818 4667Email zjuxin667@163.comObjective: Long non-coding RNAs (lncRNAs) have been identified as important players in tumorigenesis. LncRNA TMPO antisense RNA 1 (TMPO-AS1) has been shown to be involved in several tumors. However, the functional role and the underlying mechanism of TMPO-AS1 in regulating cervical cancer cell behavior remain unclear.Materials and Methods: Expression of TMPO-AS1, miR-143-3p, and ZEB1 were examined by qRT-PCR and Western blot. Cell proliferation, migration and invasion were evaluated using CCK-8 assay and Transwell migration and invasion assays, respectively. Luciferase reporter assay was performed to investigate the interaction miR-143-3p and TMPO-AS1 or ZEB1.Results: TMPO-AS1 was highly expressed in cervical cancer cells. Furthermore, TMPO-AS1 overexpression significantly promoted C-33A cell proliferation, migration, and invasion. In contrast, TMPO-AS1 silencing inhibited SiHa cell proliferation, migration, and invasion. Mechanistically, TMPO-AS1 acted as a sponge of miR-143-3p to elevate expression of zinc finger E-box binding homeobox 1 (ZEB1), a target of miR-143-3p, and thereby promoted C-33A cell proliferation, migration, and invasion. Further assays showed that TMPO-AS1 knockdown inhibited cervical cancer cell tumorigenesis in vivo.Conclusion: TMPO-AS1 promotes cervical cancer cell proliferation, migration, and invasion by regulating the miR-143-3p/ZEB1 axis.Keywords: cervical cancer, TMPO-AS1, miR-143-3p, ZEB1https://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cervical-cancer-cell-proliferati-peer-reviewed-article-CMARcervical cancertmpo-as1mir-143-3pzeb1 |
| spellingShingle | Gang X Yuan M Zhang J Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis Cancer Management and Research cervical cancer tmpo-as1 mir-143-3p zeb1 |
| title | Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis |
| title_full | Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis |
| title_fullStr | Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis |
| title_full_unstemmed | Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis |
| title_short | Long Non-Coding RNA TMPO-AS1 Promotes Cervical Cancer Cell Proliferation, Migration, and Invasion by Regulating miR-143-3p/ZEB1 Axis |
| title_sort | long non coding rna tmpo as1 promotes cervical cancer cell proliferation migration and invasion by regulating mir 143 3p zeb1 axis |
| topic | cervical cancer tmpo-as1 mir-143-3p zeb1 |
| url | https://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cervical-cancer-cell-proliferati-peer-reviewed-article-CMAR |
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