A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli

A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli

Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a stati...

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Bibliographic Details
Main Authors: Mohammad Ebrahimifard, Mohammad Mahdi Forghanifard, Ahad Yamchi, Vajiheh Zarrinpour, Mahrokh Sharbatkhari
Format: Article
Language:English
Published: SpringerOpen 2022-12-01
Series:AMB Express
Subjects:
Recombinant enterokinase
Escherichia coli
Response surface methodology
Protein folding
Affinity chromatography
Online Access:https://doi.org/10.1186/s13568-022-01504-9
Description
Summary:Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a statistical method (RSM) to analyze a number of treatments and prediction of the most effective ones to optimize the expression system.
ISSN:2191-0855

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