A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli
Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a stati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-12-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-022-01504-9 |
| _version_ | 1797973476133306368 |
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| author | Mohammad Ebrahimifard Mohammad Mahdi Forghanifard Ahad Yamchi Vajiheh Zarrinpour Mahrokh Sharbatkhari |
| author_facet | Mohammad Ebrahimifard Mohammad Mahdi Forghanifard Ahad Yamchi Vajiheh Zarrinpour Mahrokh Sharbatkhari |
| author_sort | Mohammad Ebrahimifard |
| collection | DOAJ |
| description | Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a statistical method (RSM) to analyze a number of treatments and prediction of the most effective ones to optimize the expression system. |
| first_indexed | 2024-04-11T04:05:08Z |
| format | Article |
| id | doaj.art-58871109abdf4824b30107de9f5602d7 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-11T04:05:08Z |
| publishDate | 2022-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-58871109abdf4824b30107de9f5602d72023-01-01T12:28:24ZengSpringerOpenAMB Express2191-08552022-12-011211910.1186/s13568-022-01504-9A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coliMohammad Ebrahimifard0Mohammad Mahdi Forghanifard1Ahad Yamchi2Vajiheh Zarrinpour3Mahrokh Sharbatkhari4Department of Biology, Damghan Branch, Islamic Azad UniversityDepartment of Biology, Damghan Branch, Islamic Azad UniversityDepartment of Biotechnology, Gorgan University of Agricultural Sciences and Natural ResourcesDepartment of Biology, Damghan Branch, Islamic Azad UniversityResearch and Development Division of Arya Tina Gene CompanyKey points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a statistical method (RSM) to analyze a number of treatments and prediction of the most effective ones to optimize the expression system.https://doi.org/10.1186/s13568-022-01504-9Recombinant enterokinaseEscherichia coliResponse surface methodologyProtein foldingAffinity chromatography |
| spellingShingle | Mohammad Ebrahimifard Mohammad Mahdi Forghanifard Ahad Yamchi Vajiheh Zarrinpour Mahrokh Sharbatkhari A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli AMB Express Recombinant enterokinase Escherichia coli Response surface methodology Protein folding Affinity chromatography |
| title | A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli |
| title_full | A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli |
| title_fullStr | A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli |
| title_full_unstemmed | A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli |
| title_short | A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli |
| title_sort | simple and efficient method for cytoplasmic production of human enterokinase light chain in e coli |
| topic | Recombinant enterokinase Escherichia coli Response surface methodology Protein folding Affinity chromatography |
| url | https://doi.org/10.1186/s13568-022-01504-9 |
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