A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli
Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a stati...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-12-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-022-01504-9 |