The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients
<p><strong>Objective</strong> Multiple sclerosis (MS) is a mainly cell-mediated autoimmune demyelinating disease in central nervous system (CNS), characterized by inflammatory demyelinating and infiltration of mononuclear cells around microvessels in CNS. It has been shown that...
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| Format: | Article |
| Jezik: | English |
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Tianjin Huanhu Hospital
2014-10-01
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| Serija: | Chinese Journal of Contemporary Neurology and Neurosurgery |
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| Online dostop: | http://www.cjcnn.org/index.php/cjcnn/article/view/1056 |
| _version_ | 1828393573081415680 |
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| author | Li-yan QIAO Xian-hao XU Shi-fang HOU |
| author_facet | Li-yan QIAO Xian-hao XU Shi-fang HOU |
| author_sort | Li-yan QIAO |
| collection | DOAJ |
| description | <p><strong>Objective</strong> Multiple sclerosis (MS) is a mainly cell-mediated autoimmune demyelinating disease in central nervous system (CNS), characterized by inflammatory demyelinating and infiltration of mononuclear cells around microvessels in CNS. It has been shown that MS is caused by the imbalance between T helper cell 1 (Th1) and Th2 or between inflammatory cytokines and anti-inflammatory cytokines. However, the profile of cytokine according to the published data is contradictory. This study is to evaluate the status of cytokines from mononuclear T cells in MS patients and try to provide clues for clinical diagnosis and treatment. <strong>Methods</strong> Enzyme-linked immunospot assay (ELISPOT) was used to test the spontaneous and antigen-specific [concanavalin A (ConA), myelin basic protein (MBP) and acetyleholine receptor (AChR)] Th1-related cytokine interferon-γ (IFN-γ) and Th2-related cytokines interleukin-4 (IL-4), IL-10 in the peripheral blood mononuclear cells in MS patients, who had not received any immunological treatment over the last 3 months. <strong>Results</strong> Compared with normal controls and patients with non-immune neurological diseases, MBP specific IL-4, IL-10 and IFN-γ of MS patients increased significantly (<em>P</em> = 0.000, for all). In addition, MBP specific IFN-γ level of MS patients increased signicantly in acute or exacerbating phase when compared with that in stable phase (<em>P</em> = 0.002), while MBP specific IL-4 and IL-10 levels did not differ significantly (<em>P</em> > 0.05, for all). <strong>Conclusions</strong> The examinations of IL-4, IL-10 and IFN-γ cytokines using ELISPOT are helpful for the differential diagnosis and the disease course of MS.</p><p> </p><p><strong>doi:</strong> 10.3969/j.issn.1672-6731.2014.10.008</p> |
| first_indexed | 2024-12-10T07:41:17Z |
| format | Article |
| id | doaj.art-588e90b4b27f4d2a9d0d10b7da29e48d |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-12-10T07:41:17Z |
| publishDate | 2014-10-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-588e90b4b27f4d2a9d0d10b7da29e48d2022-12-22T01:57:18ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312014-10-0114108678721052The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patientsLi-yan QIAO0Xian-hao XU1Shi-fang HOU2Department of Neurology, Tsinghua University Yuquan Hospital, Beijing 100040, ChinaDepartment of Neurology, Beijing Hospital, Beijing 100730, ChinaDepartment of Neurology, Beijing Hospital, Beijing 100730, China<p><strong>Objective</strong> Multiple sclerosis (MS) is a mainly cell-mediated autoimmune demyelinating disease in central nervous system (CNS), characterized by inflammatory demyelinating and infiltration of mononuclear cells around microvessels in CNS. It has been shown that MS is caused by the imbalance between T helper cell 1 (Th1) and Th2 or between inflammatory cytokines and anti-inflammatory cytokines. However, the profile of cytokine according to the published data is contradictory. This study is to evaluate the status of cytokines from mononuclear T cells in MS patients and try to provide clues for clinical diagnosis and treatment. <strong>Methods</strong> Enzyme-linked immunospot assay (ELISPOT) was used to test the spontaneous and antigen-specific [concanavalin A (ConA), myelin basic protein (MBP) and acetyleholine receptor (AChR)] Th1-related cytokine interferon-γ (IFN-γ) and Th2-related cytokines interleukin-4 (IL-4), IL-10 in the peripheral blood mononuclear cells in MS patients, who had not received any immunological treatment over the last 3 months. <strong>Results</strong> Compared with normal controls and patients with non-immune neurological diseases, MBP specific IL-4, IL-10 and IFN-γ of MS patients increased significantly (<em>P</em> = 0.000, for all). In addition, MBP specific IFN-γ level of MS patients increased signicantly in acute or exacerbating phase when compared with that in stable phase (<em>P</em> = 0.002), while MBP specific IL-4 and IL-10 levels did not differ significantly (<em>P</em> > 0.05, for all). <strong>Conclusions</strong> The examinations of IL-4, IL-10 and IFN-γ cytokines using ELISPOT are helpful for the differential diagnosis and the disease course of MS.</p><p> </p><p><strong>doi:</strong> 10.3969/j.issn.1672-6731.2014.10.008</p>http://www.cjcnn.org/index.php/cjcnn/article/view/1056Multiple sclerosisInterleukin-4Interleukin-10Interferon type ⅡMyelin proteins |
| spellingShingle | Li-yan QIAO Xian-hao XU Shi-fang HOU The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients Chinese Journal of Contemporary Neurology and Neurosurgery Multiple sclerosis Interleukin-4 Interleukin-10 Interferon type Ⅱ Myelin proteins |
| title | The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients |
| title_full | The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients |
| title_fullStr | The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients |
| title_full_unstemmed | The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients |
| title_short | The study on secretion of cytokine interleukin-4, interleukin-10 and interferon-γ in peripheral blood of multiple sclerosis patients |
| title_sort | study on secretion of cytokine interleukin 4 interleukin 10 and interferon γ in peripheral blood of multiple sclerosis patients |
| topic | Multiple sclerosis Interleukin-4 Interleukin-10 Interferon type Ⅱ Myelin proteins |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/1056 |
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