Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析)
应用高效阴离子交换色谱-脉冲安培检测器联用技术，研究了牛蒡多糖组分的检测方法和条件.以牛蒡为原料，采用水提醇沉法提取牛蒡粗多糖，并对其工艺进行探讨.牛蒡粗多糖经超声波酸水解处理后进行离子色谱分析，其最佳色谱条件为：以12 mmol • L-1 NaOH溶液为淋洗液，流动相流速为1. 0 mL • min-1，Carbo PaC PA10 (250 mm×4 mm i. d.)为分析柱，进样量25 µL.结果表明，牛蒡多糖中主要含有阿拉伯糖、果糖、葡萄糖和半乳糖，在优化的色谱条件下，各种单糖组分在一定浓度范围内线性关系良好，能很好地分离和准确测定.该方法操作简便、灵敏度高、重现性好....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2014-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2014.05.011 |
| _version_ | 1797235911744815104 |
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| author | ZHENGYimei(郑一美) WENZhengru(温正如) SONGYupeng(宋宇鹏) ZHOUFufu(周福富) LUOXiaohui(罗小会) |
| author_facet | ZHENGYimei(郑一美) WENZhengru(温正如) SONGYupeng(宋宇鹏) ZHOUFufu(周福富) LUOXiaohui(罗小会) |
| author_sort | ZHENGYimei(郑一美) |
| collection | DOAJ |
| description | 应用高效阴离子交换色谱-脉冲安培检测器联用技术，研究了牛蒡多糖组分的检测方法和条件.以牛蒡为原料，采用水提醇沉法提取牛蒡粗多糖，并对其工艺进行探讨.牛蒡粗多糖经超声波酸水解处理后进行离子色谱分析，其最佳色谱条件为：以12 mmol • L-1 NaOH溶液为淋洗液，流动相流速为1. 0 mL • min-1，Carbo PaC PA10 (250 mm×4 mm i. d.)为分析柱，进样量25 µL.结果表明，牛蒡多糖中主要含有阿拉伯糖、果糖、葡萄糖和半乳糖，在优化的色谱条件下，各种单糖组分在一定浓度范围内线性关系良好，能很好地分离和准确测定.该方法操作简便、灵敏度高、重现性好. |
| first_indexed | 2024-04-24T16:55:29Z |
| format | Article |
| id | doaj.art-589031b683664c4b80bd0b5903f151d7 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:55:29Z |
| publishDate | 2014-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-589031b683664c4b80bd0b5903f151d72024-03-29T01:58:33ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972014-09-0141553754110.3785/j.issn.1008-9497.2014.05.011Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析)ZHENGYimei(郑一美)0WENZhengru(温正如)1SONGYupeng(宋宇鹏)2ZHOUFufu(周福富)3LUOXiaohui(罗小会)4Zhejiang Jinhua College of Vocation and Technology, Jinhua 321000, Zhejiang Province, China(浙江金华职业技术学院，浙江 金华 321000)Zhejiang Jinhua College of Vocation and Technology, Jinhua 321000, Zhejiang Province, China(浙江金华职业技术学院，浙江 金华 321000)Zhejiang Jinhua College of Vocation and Technology, Jinhua 321000, Zhejiang Province, China(浙江金华职业技术学院，浙江 金华 321000)Zhejiang Jinhua College of Vocation and Technology, Jinhua 321000, Zhejiang Province, China(浙江金华职业技术学院，浙江 金华 321000)Zhejiang Jinhua College of Vocation and Technology, Jinhua 321000, Zhejiang Province, China(浙江金华职业技术学院，浙江 金华 321000)应用高效阴离子交换色谱-脉冲安培检测器联用技术，研究了牛蒡多糖组分的检测方法和条件.以牛蒡为原料，采用水提醇沉法提取牛蒡粗多糖，并对其工艺进行探讨.牛蒡粗多糖经超声波酸水解处理后进行离子色谱分析，其最佳色谱条件为：以12 mmol • L-1 NaOH溶液为淋洗液，流动相流速为1. 0 mL • min-1，Carbo PaC PA10 (250 mm×4 mm i. d.)为分析柱，进样量25 µL.结果表明，牛蒡多糖中主要含有阿拉伯糖、果糖、葡萄糖和半乳糖，在优化的色谱条件下，各种单糖组分在一定浓度范围内线性关系良好，能很好地分离和准确测定.该方法操作简便、灵敏度高、重现性好.https://doi.org/10.3785/j.issn.1008-9497.2014.05.011牛蒡多糖组份提取高效阴离子交换色谱脉冲安培 |
| spellingShingle | ZHENGYimei(郑一美) WENZhengru(温正如) SONGYupeng(宋宇鹏) ZHOUFufu(周福富) LUOXiaohui(罗小会) Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) Zhejiang Daxue xuebao. Lixue ban 牛蒡 多糖组份 提取 高效阴离子交换色谱 脉冲安培 |
| title | Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) |
| title_full | Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) |
| title_fullStr | Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) |
| title_full_unstemmed | Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) |
| title_short | Analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection(牛蒡多糖组分的高效阴离子交换-脉冲安培分析) |
| title_sort | analysis of burdock polysaccharide components by high performance anion exchange chromatography coupled with pulsed amperometric detection 牛蒡多糖组分的高效阴离子交换 脉冲安培分析 |
| topic | 牛蒡 多糖组份 提取 高效阴离子交换色谱 脉冲安培 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2014.05.011 |
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