Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study
Regardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/591 |
| _version_ | 1797625618244829184 |
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| author | Erenildo Ferreira de Macedo Nivia Salles Santos Lucca Silva Nascimento Raphaël Mathey Sophie Brenet Matheus Sacilotto de Moura Yanxia Hou Dayane Batista Tada |
| author_facet | Erenildo Ferreira de Macedo Nivia Salles Santos Lucca Silva Nascimento Raphaël Mathey Sophie Brenet Matheus Sacilotto de Moura Yanxia Hou Dayane Batista Tada |
| author_sort | Erenildo Ferreira de Macedo |
| collection | DOAJ |
| description | Regardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle and the biochemical environment, numerous studies have provided evidence that direct contact between NPs and biomolecules or cell membranes leads to cell inactivation or damage and may be a primary mechanism for cytotoxicity. In such a context, this work focused on developing a fast and accurate method to characterize the interaction between NPs, proteins and lipidic membranes by surface plasmon resonance imaging (SPRi) technique. The interaction of gold NPs with mimetic membranes was evaluated by monitoring the variation of reflectivity after several consecutive gold NPs injections on the lipidic membranes prepared on the SPRi biochip. The interaction on the membranes with varied lipidic composition was compared regarding the total surface concentration density of gold NPs adsorbed on them. Then, the interaction of gold and silver NPs with blood proteins was analyzed regarding their kinetic profile of the association/dissociation and dissociation constants (k<sub>off</sub>). The surface concentration density on the membrane composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and cholesterol (POPC/cholesterol) was 2.5 times higher than the value found after the injections of gold NPs on POPC only or with dimethyldioctadecylammonium (POPC/DDAB). Regarding the proteins, gold NPs showed preferential binding to fibrinogen resulting in a value of the variation of reflectivity that was 8 times higher than the value found for the other proteins. Differently, silver NPs showed similar interaction on all the tested proteins but with a variation of reflectivity on immunoglobulin G (IgG) 2 times higher than the value found for the other tested proteins. |
| first_indexed | 2024-03-11T09:58:51Z |
| format | Article |
| id | doaj.art-5890c9475db2457984cd3df229568f6b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:58:51Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5890c9475db2457984cd3df229568f6b2023-11-16T15:35:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124159110.3390/ijms24010591Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance StudyErenildo Ferreira de Macedo0Nivia Salles Santos1Lucca Silva Nascimento2Raphaël Mathey3Sophie Brenet4Matheus Sacilotto de Moura5Yanxia Hou6Dayane Batista Tada7Laboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, BrazilLaboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, BrazilLaboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, BrazilUniversity Grenoble Alpes, CEA, CNRS, IRIG-SYMMES, 38000 Grenoble, FranceUniversity Grenoble Alpes, CEA, CNRS, IRIG-SYMMES, 38000 Grenoble, FranceLaboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, BrazilUniversity Grenoble Alpes, CEA, CNRS, IRIG-SYMMES, 38000 Grenoble, FranceLaboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, BrazilRegardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle and the biochemical environment, numerous studies have provided evidence that direct contact between NPs and biomolecules or cell membranes leads to cell inactivation or damage and may be a primary mechanism for cytotoxicity. In such a context, this work focused on developing a fast and accurate method to characterize the interaction between NPs, proteins and lipidic membranes by surface plasmon resonance imaging (SPRi) technique. The interaction of gold NPs with mimetic membranes was evaluated by monitoring the variation of reflectivity after several consecutive gold NPs injections on the lipidic membranes prepared on the SPRi biochip. The interaction on the membranes with varied lipidic composition was compared regarding the total surface concentration density of gold NPs adsorbed on them. Then, the interaction of gold and silver NPs with blood proteins was analyzed regarding their kinetic profile of the association/dissociation and dissociation constants (k<sub>off</sub>). The surface concentration density on the membrane composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and cholesterol (POPC/cholesterol) was 2.5 times higher than the value found after the injections of gold NPs on POPC only or with dimethyldioctadecylammonium (POPC/DDAB). Regarding the proteins, gold NPs showed preferential binding to fibrinogen resulting in a value of the variation of reflectivity that was 8 times higher than the value found for the other proteins. Differently, silver NPs showed similar interaction on all the tested proteins but with a variation of reflectivity on immunoglobulin G (IgG) 2 times higher than the value found for the other tested proteins.https://www.mdpi.com/1422-0067/24/1/591nanoparticlesprotein coronamembranessurface plasmon resonance imagingbiomolecular interactionsblood serum proteins |
| spellingShingle | Erenildo Ferreira de Macedo Nivia Salles Santos Lucca Silva Nascimento Raphaël Mathey Sophie Brenet Matheus Sacilotto de Moura Yanxia Hou Dayane Batista Tada Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study International Journal of Molecular Sciences nanoparticles protein corona membranes surface plasmon resonance imaging biomolecular interactions blood serum proteins |
| title | Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study |
| title_full | Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study |
| title_fullStr | Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study |
| title_full_unstemmed | Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study |
| title_short | Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study |
| title_sort | interaction between nanoparticles membranes and proteins a surface plasmon resonance study |
| topic | nanoparticles protein corona membranes surface plasmon resonance imaging biomolecular interactions blood serum proteins |
| url | https://www.mdpi.com/1422-0067/24/1/591 |
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