Alignment of cell division axes in directed epithelial cell migration
Cell division is an essential dynamic event in tissue remodeling during wound healing, cancer and embryogenesis. In collective migration, tensile stresses affect cell shape and polarity, hence, the orientation of the cell division axis is expected to depend on cellular flow patterns. Here, we study...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2014-01-01
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| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/16/11/115005 |
| _version_ | 1797751510435627008 |
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| author | Anna-Kristina Marel Nils Podewitz Matthias Zorn Joachim Oskar Rädler Jens Elgeti |
| author_facet | Anna-Kristina Marel Nils Podewitz Matthias Zorn Joachim Oskar Rädler Jens Elgeti |
| author_sort | Anna-Kristina Marel |
| collection | DOAJ |
| description | Cell division is an essential dynamic event in tissue remodeling during wound healing, cancer and embryogenesis. In collective migration, tensile stresses affect cell shape and polarity, hence, the orientation of the cell division axis is expected to depend on cellular flow patterns. Here, we study the degree of orientation of cell division axes in migrating and resting epithelial cell sheets. We use microstructured channels to create a defined scenario of directed cell invasion and compare this situation to resting but proliferating cell monolayers. In experiments, we find a strong alignment of the axis due to directed flow while resting sheets show very weak global order, but local flow gradients still correlate strongly with the cell division axis. We compare experimental results with a previously published mesoscopic particle based simulation model. Most of the observed effects are reproduced by the simulations. |
| first_indexed | 2024-03-12T16:49:32Z |
| format | Article |
| id | doaj.art-58938370c12043e2bcb0015bdedaa976 |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:49:32Z |
| publishDate | 2014-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-58938370c12043e2bcb0015bdedaa9762023-08-08T11:24:43ZengIOP PublishingNew Journal of Physics1367-26302014-01-01161111500510.1088/1367-2630/16/11/115005Alignment of cell division axes in directed epithelial cell migrationAnna-Kristina Marel0Nils Podewitz1Matthias Zorn2Joachim Oskar Rädler3Jens Elgeti4Fakultät für Physik and Center for NanoScience, Ludwig-Maximilians-Universität , Geschwister-Scholl-Platz 1, D-80539 München, GermanyTheoretical Soft Matter and Biophysics, Institute of Complex Systems , Forschungszentrum Jülich, D-52425 Jülich, GermanyFakultät für Physik and Center for NanoScience, Ludwig-Maximilians-Universität , Geschwister-Scholl-Platz 1, D-80539 München, GermanyFakultät für Physik and Center for NanoScience, Ludwig-Maximilians-Universität , Geschwister-Scholl-Platz 1, D-80539 München, GermanyTheoretical Soft Matter and Biophysics, Institute of Complex Systems , Forschungszentrum Jülich, D-52425 Jülich, GermanyCell division is an essential dynamic event in tissue remodeling during wound healing, cancer and embryogenesis. In collective migration, tensile stresses affect cell shape and polarity, hence, the orientation of the cell division axis is expected to depend on cellular flow patterns. Here, we study the degree of orientation of cell division axes in migrating and resting epithelial cell sheets. We use microstructured channels to create a defined scenario of directed cell invasion and compare this situation to resting but proliferating cell monolayers. In experiments, we find a strong alignment of the axis due to directed flow while resting sheets show very weak global order, but local flow gradients still correlate strongly with the cell division axis. We compare experimental results with a previously published mesoscopic particle based simulation model. Most of the observed effects are reproduced by the simulations.https://doi.org/10.1088/1367-2630/16/11/115005tissue growthsimulationsMDCK87.19.xj87.19.R-87.55.Gh |
| spellingShingle | Anna-Kristina Marel Nils Podewitz Matthias Zorn Joachim Oskar Rädler Jens Elgeti Alignment of cell division axes in directed epithelial cell migration New Journal of Physics tissue growth simulations MDCK 87.19.xj 87.19.R- 87.55.Gh |
| title | Alignment of cell division axes in directed epithelial cell migration |
| title_full | Alignment of cell division axes in directed epithelial cell migration |
| title_fullStr | Alignment of cell division axes in directed epithelial cell migration |
| title_full_unstemmed | Alignment of cell division axes in directed epithelial cell migration |
| title_short | Alignment of cell division axes in directed epithelial cell migration |
| title_sort | alignment of cell division axes in directed epithelial cell migration |
| topic | tissue growth simulations MDCK 87.19.xj 87.19.R- 87.55.Gh |
| url | https://doi.org/10.1088/1367-2630/16/11/115005 |
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