The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation
Background. One of the results of negative influence of irradiation is the radiational injury of lungs, which manifests itself by radiational pneumonitis and pulmonary fibrosis. The medicines of phytogenous and animal origin, microelements and synthetic analogs of natural organism protection's...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy
2014-06-01
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| Series: | Морфологія |
| Subjects: | |
| Online Access: | http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14sdvoto.pdf |
| _version_ | 1818136346374438912 |
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| author | Shatov D.V. Pikalyuk V.S. Shalanin V.V. Shimkus T.S. |
| author_facet | Shatov D.V. Pikalyuk V.S. Shalanin V.V. Shimkus T.S. |
| author_sort | Shatov D.V. |
| collection | DOAJ |
| description | Background. One of the results of negative influence of irradiation is the radiational injury of lungs, which manifests itself by radiational pneumonitis and pulmonary fibrosis. The medicines of phytogenous and animal origin, microelements and synthetic analogs of natural organism protection's substances, high-molecular compounds, vaccines, cytokines and their inductors are used for early therapy of these complications. As one of such remedies one can use the xenogenic cerebrospinal fluid of cows since its usage shows good effects over the system of hematopoiesis at treatment of medullar form of acute radiation sickness. Objective. Studying the influence of xenogenic cerebrospinal fluid over the lung's parenchyma of the rats, which were exposed to single total irradiation. Methods. The research was performed on 48 mature aged (5 months) Wistar rats of both sexes. The animals were divided into following groups: the placebo group, the group, which was getting xenogenic cerebrospinal fluid, the irradiated group, which was getting placebo, and the irradiated group, which was getting cerebrospinal fluid. The lungs were later histologically processed and colored by hematoxylin-eosin and picrofucsine. During the microscopy the percentage of the areas with intact parenchyma, emphysema, dystelektases and hemorrhages, and also the intensity of the collagen fibers were estimated. Results. The triple introduction of the xenogenic cerebrospinal fluid to the animals, which were undergone the irradiation, have reduced the percentage of emphysematous areas by 8,86% (р<0,01) and the haemorrhagic areas by 2,44% (р<0,001) at the expense of increase of the percentage of intact parenchyma by 7,21% (р<0,01) and dystelectatic areas by 4,09% (р<0,001). At the tenfold introduction the percentage of the areas with the intact parenchyma has grown by 11,28% (р<0,001) and the percentage of dystelectatic areas by 4,51% (р<0,001), while the emphysematous areas decreased by 14,42% (р<0,001) and the haemorrhagic areas – by 1,39% (р<0,001). The most pronounced changes were observed at the 30-day experiment. Conclusion. The introduce of xenogenic cerebrospinal fluid to the animals, which were undergone the single total gamma irradiation by 5 Gy, leads to the decrease of the percentage of emphysematous areas and the increase of the intact parenchyma's and dystelectatic areas' percentage, which are more pronounced under the tenfold introduce of CSF (while the normal characteristics of the lung's morphometry are not reached). These changes are accompanied by reduced collagen formation in the lungs.
Citation: Shatov DV, Pikalyuk VS, Shalanin VV, Shimkus TS. [The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation]. Morphologia. 2014;8(2):77-88. Russian. |
| first_indexed | 2024-12-11T09:38:57Z |
| format | Article |
| id | doaj.art-5895aa10c96744fd9b4126638ae97908 |
| institution | Directory Open Access Journal |
| issn | 1997-9665 1997-9665 |
| language | Russian |
| last_indexed | 2024-12-11T09:38:57Z |
| publishDate | 2014-06-01 |
| publisher | Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy |
| record_format | Article |
| series | Морфологія |
| spelling | doaj.art-5895aa10c96744fd9b4126638ae979082022-12-22T01:12:45ZrusMinistry of Health of Ukraine. Dnipropetrovsk Medical AcademyМорфологія1997-96651997-96652014-06-01827788The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiationShatov D.V.0Pikalyuk V.S.1Shalanin V.V.2Shimkus T.S.3State institution "S.I.Georgievsky Crimean State Medical University", SimferopolState institution "S.I.Georgievsky Crimean State Medical University", SimferopolState institution "S.I.Georgievsky Crimean State Medical University", SimferopolState institution "S.I.Georgievsky Crimean State Medical University", SimferopolBackground. One of the results of negative influence of irradiation is the radiational injury of lungs, which manifests itself by radiational pneumonitis and pulmonary fibrosis. The medicines of phytogenous and animal origin, microelements and synthetic analogs of natural organism protection's substances, high-molecular compounds, vaccines, cytokines and their inductors are used for early therapy of these complications. As one of such remedies one can use the xenogenic cerebrospinal fluid of cows since its usage shows good effects over the system of hematopoiesis at treatment of medullar form of acute radiation sickness. Objective. Studying the influence of xenogenic cerebrospinal fluid over the lung's parenchyma of the rats, which were exposed to single total irradiation. Methods. The research was performed on 48 mature aged (5 months) Wistar rats of both sexes. The animals were divided into following groups: the placebo group, the group, which was getting xenogenic cerebrospinal fluid, the irradiated group, which was getting placebo, and the irradiated group, which was getting cerebrospinal fluid. The lungs were later histologically processed and colored by hematoxylin-eosin and picrofucsine. During the microscopy the percentage of the areas with intact parenchyma, emphysema, dystelektases and hemorrhages, and also the intensity of the collagen fibers were estimated. Results. The triple introduction of the xenogenic cerebrospinal fluid to the animals, which were undergone the irradiation, have reduced the percentage of emphysematous areas by 8,86% (р<0,01) and the haemorrhagic areas by 2,44% (р<0,001) at the expense of increase of the percentage of intact parenchyma by 7,21% (р<0,01) and dystelectatic areas by 4,09% (р<0,001). At the tenfold introduction the percentage of the areas with the intact parenchyma has grown by 11,28% (р<0,001) and the percentage of dystelectatic areas by 4,51% (р<0,001), while the emphysematous areas decreased by 14,42% (р<0,001) and the haemorrhagic areas – by 1,39% (р<0,001). The most pronounced changes were observed at the 30-day experiment. Conclusion. The introduce of xenogenic cerebrospinal fluid to the animals, which were undergone the single total gamma irradiation by 5 Gy, leads to the decrease of the percentage of emphysematous areas and the increase of the intact parenchyma's and dystelectatic areas' percentage, which are more pronounced under the tenfold introduce of CSF (while the normal characteristics of the lung's morphometry are not reached). These changes are accompanied by reduced collagen formation in the lungs. Citation: Shatov DV, Pikalyuk VS, Shalanin VV, Shimkus TS. [The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation]. Morphologia. 2014;8(2):77-88. Russian.http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14sdvoto.pdfcerebrospinal fluidparenchyma of lungsmorphometryirradiationcorrection |
| spellingShingle | Shatov D.V. Pikalyuk V.S. Shalanin V.V. Shimkus T.S. The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation Морфологія cerebrospinal fluid parenchyma of lungs morphometry irradiation correction |
| title | The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation |
| title_full | The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation |
| title_fullStr | The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation |
| title_full_unstemmed | The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation |
| title_short | The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation |
| title_sort | influence of xenogenic cerebrospinal fluid over the parenchyma of rat s lungs after single total irradiation |
| topic | cerebrospinal fluid parenchyma of lungs morphometry irradiation correction |
| url | http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14sdvoto.pdf |
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