Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction
Background: Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/2/444 |
| _version_ | 1797481145228591104 |
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| author | Suriya Prausmüller Georg Spinka Stefanie Stasek Henrike Arfsten Philipp Emanuel Bartko Georg Goliasch Martin Hülsmann Noemi Pavo |
| author_facet | Suriya Prausmüller Georg Spinka Stefanie Stasek Henrike Arfsten Philipp Emanuel Bartko Georg Goliasch Martin Hülsmann Noemi Pavo |
| author_sort | Suriya Prausmüller |
| collection | DOAJ |
| description | Background: Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection fraction (HFrEF) are scarce. The present study aims to evaluate the role of neutrophil CD11b, CD66b and CD64 expression in HFrEF. Methods: A total of 135 HFrEF patients and 43 controls were recruited. Mean fluorescence intensity of the activation/maturation markers CD11b, CD66b and CD64 was measured on neutrophils by flow cytometry. CD10 (neprilysin) expression was simultaneously determined. Results: Neutrophil CD64 expression was higher in HFrEF compared with controls, while CD11b/CD66b levels were similar. Neutrophil CD11b and CD66b showed a significant direct correlation to neutrophil CD10 expression (rs = 0.573, <i>p</i> < 0.001 and rs = 0.184, <i>p</i> = 0.033). Neutrophil CD11b and CD66b correlated inversely with heart failure severity reflected by NT-proBNP and NYHA class (NT-proBNP: rs = −0.243, <i>p</i> = 0.005 and rs = −0.250, <i>p</i> = 0.004; NYHA class: <i>p</i> = 0.032 and <i>p</i> = 0.055), whereas no association for CD64 could be found. Outcome analysis did not reveal a significant association between the expression of CD11b, CD66b and CD64 and all-cause mortality (<i>p</i> = ns). Conclusions: The results underline the potential role of neutrophils in HFrEF disease pathophysiology and risk stratification and should stimulate further research, characterizing subpopulations of neutrophils and searching for key molecules involved in the downward spiral of inflammation and heart failure. |
| first_indexed | 2024-03-09T22:11:18Z |
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| id | doaj.art-5895bcfb5f434ebc94848c252c0a4beb |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T22:11:18Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-5895bcfb5f434ebc94848c252c0a4beb2023-11-23T19:32:08ZengMDPI AGDiagnostics2075-44182022-02-0112244410.3390/diagnostics12020444Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection FractionSuriya Prausmüller0Georg Spinka1Stefanie Stasek2Henrike Arfsten3Philipp Emanuel Bartko4Georg Goliasch5Martin Hülsmann6Noemi Pavo7Department of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, AustriaBackground: Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection fraction (HFrEF) are scarce. The present study aims to evaluate the role of neutrophil CD11b, CD66b and CD64 expression in HFrEF. Methods: A total of 135 HFrEF patients and 43 controls were recruited. Mean fluorescence intensity of the activation/maturation markers CD11b, CD66b and CD64 was measured on neutrophils by flow cytometry. CD10 (neprilysin) expression was simultaneously determined. Results: Neutrophil CD64 expression was higher in HFrEF compared with controls, while CD11b/CD66b levels were similar. Neutrophil CD11b and CD66b showed a significant direct correlation to neutrophil CD10 expression (rs = 0.573, <i>p</i> < 0.001 and rs = 0.184, <i>p</i> = 0.033). Neutrophil CD11b and CD66b correlated inversely with heart failure severity reflected by NT-proBNP and NYHA class (NT-proBNP: rs = −0.243, <i>p</i> = 0.005 and rs = −0.250, <i>p</i> = 0.004; NYHA class: <i>p</i> = 0.032 and <i>p</i> = 0.055), whereas no association for CD64 could be found. Outcome analysis did not reveal a significant association between the expression of CD11b, CD66b and CD64 and all-cause mortality (<i>p</i> = ns). Conclusions: The results underline the potential role of neutrophils in HFrEF disease pathophysiology and risk stratification and should stimulate further research, characterizing subpopulations of neutrophils and searching for key molecules involved in the downward spiral of inflammation and heart failure.https://www.mdpi.com/2075-4418/12/2/444heart failureneutrophil activationCD10CD11bCD66bCD64 |
| spellingShingle | Suriya Prausmüller Georg Spinka Stefanie Stasek Henrike Arfsten Philipp Emanuel Bartko Georg Goliasch Martin Hülsmann Noemi Pavo Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction Diagnostics heart failure neutrophil activation CD10 CD11b CD66b CD64 |
| title | Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction |
| title_full | Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction |
| title_fullStr | Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction |
| title_full_unstemmed | Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction |
| title_short | Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction |
| title_sort | neutrophil activation maturation markers in chronic heart failure with reduced ejection fraction |
| topic | heart failure neutrophil activation CD10 CD11b CD66b CD64 |
| url | https://www.mdpi.com/2075-4418/12/2/444 |
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