Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice
<p>Abstract</p> <p>Background</p> <p>Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-02-01
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| Series: | Journal of Hematology & Oncology |
| Online Access: | http://www.jhoonline.org/content/2/1/6 |
| _version_ | 1811278545899487232 |
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| author | Lawrence Theodore S Lubman David M Davis Mary A Zhao Lujun Ao Xiaoping Kong Feng-Ming |
| author_facet | Lawrence Theodore S Lubman David M Davis Mary A Zhao Lujun Ao Xiaoping Kong Feng-Ming |
| author_sort | Lawrence Theodore S |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensitive (C57BL/6) and resistant mice (C3H).</p> <p>Results</p> <p>Twenty-two cytokines in the lung tissue homogenates, bronchial lavage (BAL) fluids, and serum from 3, 6, 12, 24 hrs to 1 week after 12 Gy whole lung irradiation were profiled using a microsphere-based multiplexed cytokine assay. The majority of cytokines had similar baseline levels in C57BL/6 and C3H mice, but differed significantly after radiation. Many, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and keratinocyte-derived chemokine (KC) were elevated significantly in specimens from both strains. They usually peaked at about 3–6 hrs in C57BL/6 and 6–12 hrs in C3H. At 6 hrs in lung tissue, G-CSF, IL-6, and KC increased 6, 8, and 11 fold in C57BL/6 mice, 4, 3, and 3 fold in the C3H mice, respectively. IL-6 was 10-fold higher at 6 hrs in the C57BL/6 BAL fluid than the C3H BAL fluid. MCP-1, IP-10, and IL-1α also showed some differences between strains in the lung tissue and/or serum. For the same cytokine and within the same strain of mice, there were significant linear correlations between lung tissue and BAL fluid levels (R<sup>2 </sup>ranged 0.46–0.99) and between serum and tissue (R<sup>2 </sup>ranged 0.56–0.98).</p> <p>Conclusion</p> <p>Radiation induced earlier and greater temporal changes in multiple cytokines in the pulmonary fibrosis sensitive mice. Positive correlation between serum and tissue levels suggests that blood may be used as a surrogate marker for tissue.</p> |
| first_indexed | 2024-04-13T00:36:30Z |
| format | Article |
| id | doaj.art-58972b25a811437eb16d3a04d8a7bf2a |
| institution | Directory Open Access Journal |
| issn | 1756-8722 |
| language | English |
| last_indexed | 2024-04-13T00:36:30Z |
| publishDate | 2009-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Hematology & Oncology |
| spelling | doaj.art-58972b25a811437eb16d3a04d8a7bf2a2022-12-22T03:10:18ZengBMCJournal of Hematology & Oncology1756-87222009-02-0121610.1186/1756-8722-2-6Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant miceLawrence Theodore SLubman David MDavis Mary AZhao LujunAo XiaopingKong Feng-Ming<p>Abstract</p> <p>Background</p> <p>Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensitive (C57BL/6) and resistant mice (C3H).</p> <p>Results</p> <p>Twenty-two cytokines in the lung tissue homogenates, bronchial lavage (BAL) fluids, and serum from 3, 6, 12, 24 hrs to 1 week after 12 Gy whole lung irradiation were profiled using a microsphere-based multiplexed cytokine assay. The majority of cytokines had similar baseline levels in C57BL/6 and C3H mice, but differed significantly after radiation. Many, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and keratinocyte-derived chemokine (KC) were elevated significantly in specimens from both strains. They usually peaked at about 3–6 hrs in C57BL/6 and 6–12 hrs in C3H. At 6 hrs in lung tissue, G-CSF, IL-6, and KC increased 6, 8, and 11 fold in C57BL/6 mice, 4, 3, and 3 fold in the C3H mice, respectively. IL-6 was 10-fold higher at 6 hrs in the C57BL/6 BAL fluid than the C3H BAL fluid. MCP-1, IP-10, and IL-1α also showed some differences between strains in the lung tissue and/or serum. For the same cytokine and within the same strain of mice, there were significant linear correlations between lung tissue and BAL fluid levels (R<sup>2 </sup>ranged 0.46–0.99) and between serum and tissue (R<sup>2 </sup>ranged 0.56–0.98).</p> <p>Conclusion</p> <p>Radiation induced earlier and greater temporal changes in multiple cytokines in the pulmonary fibrosis sensitive mice. Positive correlation between serum and tissue levels suggests that blood may be used as a surrogate marker for tissue.</p>http://www.jhoonline.org/content/2/1/6 |
| spellingShingle | Lawrence Theodore S Lubman David M Davis Mary A Zhao Lujun Ao Xiaoping Kong Feng-Ming Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice Journal of Hematology & Oncology |
| title | Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| title_full | Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| title_fullStr | Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| title_full_unstemmed | Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| title_short | Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| title_sort | radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice |
| url | http://www.jhoonline.org/content/2/1/6 |
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