Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria
The article presents the results of the new developed disinfectant “Biolide” research for use in poultry and other sectors of agriculture, because today the problem of developing cheap and effective disinfectants remains relevant. In recent years, the poultry industry has a tendency to rapid develop...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Animal Biology NAAS
2021-12-01
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| Series: | Біологія тварин |
| Subjects: | |
| Online Access: | https://aminbiol.com.ua/index.php/182-archive/bt-23-4-2021/1964-efficiency-of-biolide-disinfectant-working-solutions-for-action-on-gram-negative-and-gram-positive-bacteria |
| _version_ | 1797676952741478400 |
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| author | O. N. Chechet V. L. Kovalenko T. O. Garkavenko O. I. Gorbatyuk T. H. Kozytska |
| author_facet | O. N. Chechet V. L. Kovalenko T. O. Garkavenko O. I. Gorbatyuk T. H. Kozytska |
| author_sort | O. N. Chechet |
| collection | DOAJ |
| description | The article presents the results of the new developed disinfectant “Biolide” research for use in poultry and other sectors of agriculture, because today the problem of developing cheap and effective disinfectants remains relevant. In recent years, the poultry industry has a tendency to rapid development, since the population’s demands for poultry products have increased markedly. With an integrated approach to the production of chicken meat, it is possible to increase the productivity of poultry enterprises, energy efficiency and reduce the cost of production. When applying such an integrated approach, one of the important roles is played by the provision of high-quality disinfection with effective and inexpensive means. In connection with the relevance of the development of new effective disinfectants, the main purpose of the research was to determine the effectiveness of working solutions in concentrations of 0.1; 0.2; 0.25 and 0.5% for gram-negative E. coli ATCC 25922 and gram-positive S. aureus ATCC 25923 for different periods of time — 20, 30, 60 and 120 min. after the simulation of protein contamination. Test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 in lyophilized form were stored in a refrigerator at a temperature –70±5°C. By replacing them on nutrient media, metabolic processes were restored and their correspondence to the main typical properties for this type of pathogens was checked. Simulation of protein contamination was carried out using sterile inactivated blood serum of cattle in the amount of 40.0% to the volume of the used bacterial suspension. In laboratory tests, smooth surfaces of tiles with an area 100 cm2 were used as test objects. The analysis of the obtained research results showed the high efficiency of 0.5% working solutions of the new disinfectant “Biolide” when exposed to test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 for 60 min., since this concentration of the working disinfectant solution and the exposure time ensured the destruction by 99.99–100.0% of gram-negative and gram-positive microorganisms when imitating protein contamination of test objects. |
| first_indexed | 2024-03-11T22:37:55Z |
| format | Article |
| id | doaj.art-5899468dd6a742a8bfca903fc12480d7 |
| institution | Directory Open Access Journal |
| issn | 1681-0015 2313-2191 |
| language | English |
| last_indexed | 2024-03-11T22:37:55Z |
| publishDate | 2021-12-01 |
| publisher | Institute of Animal Biology NAAS |
| record_format | Article |
| series | Біологія тварин |
| spelling | doaj.art-5899468dd6a742a8bfca903fc12480d72023-09-22T11:44:18ZengInstitute of Animal Biology NAASБіологія тварин1681-00152313-21912021-12-01234667210.15407/animbiol23.04.066Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteriaO. N. Chechet0https://orcid.org/0000-0001-5099-5577V. L. Kovalenko1https://orcid.org/0000-0002-2416-5219T. O. Garkavenko2https://orcid.org/0000-0002-3398-6836O. I. Gorbatyuk3https://orcid.org/0000-0002-0573-2089T. H. Kozytska4State Research Institute for Laboratory Diagnostics and Veterinary Sanitary ExaminationState Scientific Control Institute of Biotechnology and Strains of MicroorganismsState Research Institute for Laboratory Diagnostics and Veterinary Sanitary ExaminationState Research Institute for Laboratory Diagnostics and Veterinary Sanitary ExaminationState Research Institute for Laboratory Diagnostics and Veterinary Sanitary ExaminationThe article presents the results of the new developed disinfectant “Biolide” research for use in poultry and other sectors of agriculture, because today the problem of developing cheap and effective disinfectants remains relevant. In recent years, the poultry industry has a tendency to rapid development, since the population’s demands for poultry products have increased markedly. With an integrated approach to the production of chicken meat, it is possible to increase the productivity of poultry enterprises, energy efficiency and reduce the cost of production. When applying such an integrated approach, one of the important roles is played by the provision of high-quality disinfection with effective and inexpensive means. In connection with the relevance of the development of new effective disinfectants, the main purpose of the research was to determine the effectiveness of working solutions in concentrations of 0.1; 0.2; 0.25 and 0.5% for gram-negative E. coli ATCC 25922 and gram-positive S. aureus ATCC 25923 for different periods of time — 20, 30, 60 and 120 min. after the simulation of protein contamination. Test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 in lyophilized form were stored in a refrigerator at a temperature –70±5°C. By replacing them on nutrient media, metabolic processes were restored and their correspondence to the main typical properties for this type of pathogens was checked. Simulation of protein contamination was carried out using sterile inactivated blood serum of cattle in the amount of 40.0% to the volume of the used bacterial suspension. In laboratory tests, smooth surfaces of tiles with an area 100 cm2 were used as test objects. The analysis of the obtained research results showed the high efficiency of 0.5% working solutions of the new disinfectant “Biolide” when exposed to test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 for 60 min., since this concentration of the working disinfectant solution and the exposure time ensured the destruction by 99.99–100.0% of gram-negative and gram-positive microorganisms when imitating protein contamination of test objects.https://aminbiol.com.ua/index.php/182-archive/bt-23-4-2021/1964-efficiency-of-biolide-disinfectant-working-solutions-for-action-on-gram-negative-and-gram-positive-bacteriaдезінфекційний засіб «біолайд»робочі розчинибілкова забрудненістьтестова культура e. сoli атсс 25922тестова культура s. aureus атсс 25923тест-об’єкти“biolide” disinfectantworking solutionsprotein contaminatione. coli atcc 25922 test cultures. aureus atcc 25923 test culturetest objects |
| spellingShingle | O. N. Chechet V. L. Kovalenko T. O. Garkavenko O. I. Gorbatyuk T. H. Kozytska Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria Біологія тварин дезінфекційний засіб «біолайд» робочі розчини білкова забрудненість тестова культура e. сoli атсс 25922 тестова культура s. aureus атсс 25923 тест-об’єкти “biolide” disinfectant working solutions protein contamination e. coli atcc 25922 test culture s. aureus atcc 25923 test culture test objects |
| title | Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria |
| title_full | Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria |
| title_fullStr | Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria |
| title_full_unstemmed | Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria |
| title_short | Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria |
| title_sort | efficiency of biolide disinfectant working solutions for action on gram negative and gram positive bacteria |
| topic | дезінфекційний засіб «біолайд» робочі розчини білкова забрудненість тестова культура e. сoli атсс 25922 тестова культура s. aureus атсс 25923 тест-об’єкти “biolide” disinfectant working solutions protein contamination e. coli atcc 25922 test culture s. aureus atcc 25923 test culture test objects |
| url | https://aminbiol.com.ua/index.php/182-archive/bt-23-4-2021/1964-efficiency-of-biolide-disinfectant-working-solutions-for-action-on-gram-negative-and-gram-positive-bacteria |
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